Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

1989 ◽  
Vol 107 (3-4) ◽  
pp. 315-322 ◽  
Author(s):  
G. Gerna ◽  
Antonella Sarasini ◽  
Maria Torsellini ◽  
Angela di Matteo ◽  
F. Baldanti ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa

scholarly journals Production and Characterization of Two Monoclonal Antibodies Specific for Plasmopara halstedii

2000 ◽  
Vol 66 (8) ◽  
pp. 3277-3282 ◽  
Author(s):  
S. Bouterige ◽  
R. Robert ◽  
J. P. Bouchara ◽  
A. Marot-Leblond ◽  
V. Molinero ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Detection System ◽  
Sandwich Elisa ◽  
Plasmopara Viticola ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasmopara Halstedii ◽  
Race 1 ◽  
Molecular Masses ◽  
Fungal Antigens

ABSTRACT Sunflower downy mildew, caused by the fungus Plasmopara halstedii, is a potentially devastating disease. We produced two monoclonal antibodies (MAbs) (12C9 and 18E2) by immunizing mice with a partially purified extract of P. halstedii race 1. Both MAbs detected in enzyme-linked immunosorbent assay (ELISA) all races ofP. halstedii present in France. No cross-reactions were observed with Plasmopara viticola or with other fungi commonly associated with sunflowers. Both MAbs recognized the same three fungal antigens with molecular masses of 68, 140, and 192 kDa. However, the epitopes on the fungal antigens were distinct and repetitive. Seed homogenates from infected plants were incubated in wells coated with MAb 18E2. This resulted in the trapping of P. halstedii antigens that were identified with biotinylated MAb 12C9. No reactions were seen with seed homogenates from healthy plants. Thus, our results suggest that these MAbs might be used to develop a sandwich ELISA detection system for P. halstedii in infected seeds.

Development and Characterization of Six Monoclonal Antibodies to Hemagglutinin-70 ofClostridium botulinumand Their Application in a Sandwich ELISA

2013 ◽  
Vol 32 (1) ◽  
pp. 6-15 ◽  
Author(s):  
Miles C. Scotcher ◽  
Luisa W. Cheng ◽  
Kathryn Ching ◽  
Jeffery McGarvey ◽  
Robert Hnasko ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa

scholarly journals Generation and characterization of monoclonal antibodies against mature hepcidin and its application to neutralization and quantitative alteration assay

2021 ◽  
Vol 85 (2) ◽  
pp. 340-350
Author(s):  
Shinji Sakamoto ◽  
Mika Kirinashizawa ◽  
Yumi Mohara ◽  
Yoshihiro Watanabe
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa ◽  
High Sensitivity ◽  
Cellular Membrane ◽  
Average Concentration ◽  
Cellular Iron ◽  
Capture Antibody ◽  
Quantitative Alteration ◽  
Detection Reagent

ABSTRACT Hepcidin regulates the quantity of ferroportin (FPN) on cellular membrane. In our cell assay expressing ferroportin labeled with green fluorescence, FPN was internalized and degraded only after treatment with hepcidin-25, not hepcidin-22 or hepcidin-20, leading to accumulation of cellular iron. Thus we generated murine monoclonal antibodies (mAbs) against hepcidin-25, and then characterized and validated their functions. Among them, several mAbs showed a neutralizing activity that may prevent ferroportin internalization induced by hepcidin-25. To measure hepcidin level in various fluids, mAbs specific for human and rat hepcidin-25 were selected. As for rat, a sandwich ELISA developed using clone rHN1 as capture antibody and biotinylated clone mHW1 as a detection reagent had high sensitivity, allowing for the detection of 1-100 ng/mL of hepcidin-25. Rat hepcidin-25 level in plasma was measured at an average concentration of 63.0 ng/mL in healthy condition, and at 218.2 ng/mL after stimulation of lipopolysaccharide.

scholarly journals Preparation and Characterization of Anti-GP73 Monoclonal Antibodies and Development of Double-antibody Sandwich ELISA

2015 ◽  
Vol 16 (5) ◽  
pp. 2043-2049 ◽  
Author(s):  
Qi-Wen Li ◽  
Hong-Bing Chen ◽  
Zhi-Yang Li ◽  
Peng Shen ◽  
Li-Li Qu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa ◽  
Double Antibody

scholarly journals Obtaining and Characterization of Hybridomas Producing Monoclonal Antibodies to Shiga-Like Toxins of I and II Types

2021 ◽  
pp. 83-88
Author(s):  
G. V. Kuklina ◽  
S. S. Ipatov ◽  
D. V. Pechenkin ◽  
A. V. Eremkin ◽  
A. A. Kytmanov ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Sandwich Elisa ◽  
Minimum Detectable Concentration ◽  
Myeloma Cells ◽  
Hybrid Cell Lines

Objective – obtaining and characterization of hybrid cell lines producing monoclonal antibodies against I and II types of shiga-like toxins.Materials and methods. Shiga-like toxins obtained in “48thCentral Research Institute” of Ministry of Defense of Russian Federation (Kirov), BALB/c mice, myeloma cells SP2/0-Ag14 were used in research. Immune splenocytes and SP2/0-Ag14 myeloma cells were fused according to G. Kohler and C. Milstein method in De St. Fazekas and D. Scheidegger modifcation using 50 % polyethylene glycol. Hybrid cell lines producing specifc monoclonal antibodies were cloned by limited dilutions. Hybridomas growth and producing properties were studied in vitro and in vivo. Specifc activity of immune sera, culture and ascitic fluids were studied by indirect ELISA. Monoclonal antibodies from ascitic fluids were precipitated by saturated ammonium sulfate, followed by ion exchange chromatographyResults and discussion. 8 hybridomas producing monoclonal antibodies against I and II types shiga-like toxins were obtained. Hybridomas are characterized by stable proliferation and antibody-producing activity during 10 passages in vitro and 3 passages in vivo (observation period). Obtained monoclonal antibodies can be used for ELISA detection of I and II types shiga-like toxins. Minimum detectable concentration of shiga-like toxins in sandwich ELISA is 1 ng/ml. The possibility of detecting shiga-like toxins without typical differentiation was shown when using in the enzyme immunoassay a polyreceptor mixture of monoclonal antibodies for sensitizing the plate and a polyspecifc mixture of immunoperoxidase conjugates.

Monoclonal antibodies to human cartilage oligomeric matrix protein: epitope mapping and characterization of sandwich ELISA

2003 ◽  
Vol 328 (1-2) ◽  
pp. 59-69 ◽  
Author(s):  
Vladimı́r Vilı́m ◽  
Zdeněk Vobůrka ◽  
Richard Vytášek ◽  
Ladislav Šenolt ◽  
Ilja Tchetverikov ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cartilage Oligomeric Matrix Protein ◽  
Epitope Mapping ◽  
Matrix Protein ◽  
Sandwich Elisa ◽  
Human Cartilage
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