Long-term time lapse photomicrography

1975 ◽  
Vol 1 (2) ◽  
pp. 99-102
Author(s):  
Y. T. Chang
2021 ◽  
Vol 2 (2) ◽  
pp. 92-108
Author(s):  
Maurizio Salvadori ◽  
Aris Tsalouchos

Sexual life and fertility are compromised in end stage kidney disease both in men and in women. Successful renal transplantation may rapidly recover fertility in the vast majority of patients. Pregnancy modifies anatomical and functional aspects in the kidney and represents a risk of sensitization that may cause acute rejection. Independently from the risks for the graft, pregnancy in kidney transplant may cause preeclampsia, gestational diabetes, preterm delivery, and low birth weight. The nephrologist has a fundamental role in correct counseling, in a correct evaluation of the mother conditions, and in establishing a correct time lapse between transplantation and conception. Additionally, careful attention must be given to the antirejection therapy, avoiding drugs that could be dangerous to the newborn. Due to the possibility of medical complications during pregnancy, a correct follow-up should be exerted. Even if pregnancy in transplant is considered a high risk one, several data and studies document that in the majority of patients, the long-term follow-up and outcomes for the graft may be similar to that of non-pregnant women.


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Han Wang ◽  
Gloria M. Conover ◽  
Song-I Han ◽  
James C. Sacchettini ◽  
Arum Han

AbstractAnalysis of growth and death kinetics at single-cell resolution is a key step in understanding the complexity of the nonreplicating growth phenotype of the bacterial pathogen Mycobacterium tuberculosis. Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. This technology was successfully applied to monitor the real-time growth dynamics of the fast-growing model strain Mycobacterium smegmatis (M. smegmatis) while subjected to drug treatment regimens during continuous culture for 48 h inside the microfluidic device. A clear morphological change leading to significant swelling at the poles of the bacterial membrane was observed during drug treatment. In addition, a small subpopulation of cells surviving treatment by frontline antibiotics was observed to recover and achieve robust replicative growth once regular culture media was provided, suggesting the possibility of identifying and isolating nonreplicative mycobacteria. This device is a simple, easy-to-use, and low-cost solution for studying the single-cell phenotype and growth dynamics of mycobacteria, especially during drug treatment.


1973 ◽  
Vol 51 (9) ◽  
pp. 1669-1671 ◽  
Author(s):  
M. H. Hashmi ◽  
G. Morgan-Jones

Conidium ontogeny in Wallemia sebi (Fr.) v. Arx is analyzed and illustrated by time-lapse photomicrography. The nuclear configurations occurring during conidiogenesis are described and subsequent nuclear distribution reported. The conidia are considered to be meristem arthrospores of a unique type.


Geophysics ◽  
2012 ◽  
Vol 77 (6) ◽  
pp. B287-B294 ◽  
Author(s):  
Jamie K. Pringle ◽  
Peter Styles ◽  
Claire P. Howell ◽  
Michael W. Branston ◽  
Rebecca Furner ◽  
...  

The area around the town of Northwich in Cheshire, U. K., has a long history of catastrophic ground subsidence caused by a combination of natural dissolution and collapsing abandoned mine workings within the underlying Triassic halite bedrock geology. In the village of Marston, the Trent and Mersey Canal crosses several abandoned salt mine workings and previously subsiding areas, the canal being breached by a catastrophic subsidence event in 1953. This canal section is the focus of a long-term monitoring study by conventional geotechnical topographic and microgravity surveys. Results of 20 years of topographic time-lapse surveys indicate specific areas of local subsidence that could not be predicted by available site and mine abandonment plan and shaft data. Subsidence has subsequently necessitated four phases of temporary canal bank remediation. Ten years of microgravity time-lapse data have recorded major deepening negative anomalies in specific sections that correlate with topographic data. Gravity 2D modeling using available site data found upwardly propagating voids, and associated collapse material produced a good match with observed microgravity data. Intrusive investigations have confirmed a void at the major anomaly. The advantages of undertaking such long-term studies for near-surface geophysicists, geotechnical engineers, and researchers working in other application areas are discussed.


Author(s):  
D. Fischer ◽  
P. Smith ◽  
N. Sørenes ◽  
E. Teichmann ◽  
H. Blekastad ◽  
...  

1979 ◽  
Vol 55 (3) ◽  
pp. 239-246 ◽  
Author(s):  
K. Fedra ◽  
R. Machan
Keyword(s):  

1996 ◽  
Vol 10 (4) ◽  
pp. 235-238 ◽  
Author(s):  
Rainer Weber ◽  
Rainer Keerl ◽  
Andreas Huppmann ◽  
Bernhard Schick ◽  
Wolfgang Draf

We describe a new method of documentation of the dynamics of the nature of wound healing after endonasal paranasal sinus surgery. This was not possible until now and is achieved by the computer-assisted interpolation known as morphing. We evaluated six patients with chronic polypoid sinusitis of similar extent who underwent 12 complete endonasal micro-endoscopic pansinus operations. It is difficult to describe the overlapping and meshing phases sufficiently in words. They are much more easily understood by looking at the whole process in its natural dynamic on a video screen. Wound healing after complete endonasal pansinus operation lasts over 6 months. Scar remodelling has not yet finished at this time. The following measures are recommended for decreasing postoperative granulations, edema, and swelling: minimizing the surgical trauma, long-term packing with an occlusive, nonadherent material (rubber finger packing), and topical steroids (Budesonide).


1981 ◽  
Vol 18 (1) ◽  
pp. 24-39 ◽  
Author(s):  
K. Y. Lo ◽  
Clement M. K. Yuen

Excavation of underground openings in rock relieves the in situ state of stresses, providing an initiating mechanism for time-dependent deformation in shaly rocks to occur. The construction of a permanent lining restrains the rock movement, resulting in an increase with time of pressure acting on the lining.Closed form solutions for this problem of rock structure – time interaction of circular tunnels have been developed, taking into account (a) the state of initial stresses, (b) the time-dependent properties of the lining as well as the rock, and (c) the time lapsed between excavation and lining. Expressions for lining pressures, stresses, thrusts, and moments are given for both "no slip" and "full slip" interface conditions.The effects of material parameters, lining flexibilities, and time lapse on quantities of engineering interest are studied and their implications on design illustrated.The results of analysis are compared with the field observations made in three tunnels. It is shown that the theoretical results are consistent with the observed extent and location of cracking in these tunnels.It is suggested that the methods proposed may be used for design considerations of permanent linings of tunnels in rock exhibiting significant time-dependent deformation.


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