Characterization of the extremely high molecular mass polystyrene by gel permeation chromatography

1980 ◽  
Vol 258 (6) ◽  
pp. 702-710 ◽  
Author(s):  
L. Šoltés ◽  
D. Berek ◽  
D. Mikulášová
Keyword(s):  
Molecular Mass ◽  
Gel Permeation Chromatography ◽  
High Molecular Mass ◽  
Gel Permeation

Purification and Preliminary Characterization of a Thermostable Lactase from Bacillus coagulans T242

2013 ◽  
Vol 690-693 ◽  
pp. 1362-1365
Author(s):  
Shu Juan Jiang ◽  
Guang Qing Mu ◽  
Tao Liu ◽  
Fang Qian
Keyword(s):  
Optimum Condition ◽  
Molecular Mass ◽  
Bacillus Coagulans ◽  
Gel Permeation Chromatography ◽  
Lactase Activity ◽  
High Concentration ◽  
Preliminary Characterization ◽  
Sds Page ◽  
Gel Permeation

A thermostable lactase from Bacillus coagulans T242 was subjected to purification on DEAE chromatography followed by gel permeation chromatography, then the homogenous Bacillus coagulans T242-lactase was obtained, and its molecular mass was 55.0 kDa as shown in SDS-PAGE. Analysis indicated its optimum condition was 60 C and pH 6.8 and it was stable at 40~60 C and pH6.5~7.8; Mn2+, Mg2+ and Na+ at high concentration all had marked activation on lactase activity. Kinetic constants determination revealed Bacillus coagulans T242-lactase had a strong affinity for lactose.

Molecular mass characterization of polymers with strongly interacting groups using gel permeation chromatography–light scattering detection

2002 ◽  
Vol 976 (1-2) ◽  
pp. 155-164 ◽  
Author(s):  
I.V Blagodatskikh ◽  
M.V Sutkevich ◽  
N.L Sitnikova ◽  
N.A Churochkina ◽  
T.A Pryakhina ◽  
...  
Keyword(s):  
Light Scattering ◽  
Molecular Mass ◽  
Gel Permeation Chromatography ◽  
Light Scattering Detection ◽  
Strongly Interacting ◽  
Gel Permeation

scholarly journals Molar mass determination of lignins and characterization of their polymeric structures by multi-detector gel permeation chromatography

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evamaria C. Gaugler ◽  
Wolfgang Radke ◽  
Andrew P. Vogt ◽  
Dawn A. Smith
Keyword(s):  
Molar Mass ◽  
Lithium Bromide ◽  
Gel Permeation Chromatography ◽  
Mass Determination ◽  
Organosolv Lignin ◽  
Gel Permeation ◽  
Softwood Kraft Lignin ◽  
Polymeric Structures

AbstractMolar masses, Mark-Houwink-Sakurada (MHS) exponents, and refractive index increments (dn/dc) for three lignins were determined without derivatization by multi-detector gel permeation chromatography (GPC) in dimethylformamide (DMF) with 0.05 M lithium bromide (LiBr). The lack of effectiveness of fluorescence filters on molar mass determination by GPC-multi-angle laser light scattering (MALS) was confirmed for softwood kraft lignin (Indulin AT) and revealed for mixed hardwood organosolv lignin (Alcell) as well as soda straw/grass lignin (Protobind 1000). GPC with viscometry detection confirmed that these lignins were present as compact molecules. The MHS exponent α for Indulin AT and Alcell was in the order of 0.1. Additionally, the intrinsic viscosity of Protobind 1000 for a given molar mass was much lower than that of either Alcell or Indulin AT. This is the first report of dn/dc values for these three lignins in DMF with 0.05 M LiBr.

Synthesis and chromatographic characterization of [Tc-99m]technetium-humic acid species

2000 ◽  
Vol 88 (2) ◽  
Author(s):  
D. Rößler ◽  
K. Franke ◽  
R. Süß ◽  
E. Becker ◽  
H. Kupsch
Keyword(s):  
Humic Acid ◽  
Stannous Chloride ◽  
Gel Permeation Chromatography ◽  
Paper Electrophoresis ◽  
Gel Permeation ◽  
Soil Humic Acid ◽  
Chromatographic Characterization ◽  
Ph Dependent ◽  
Layer Chromatography

A natural moor soil humic acid (HA) was labeled with Tc-99m via reduction of pertechnetate with stannous chloride. The humic acid species obtained were characterized by thin layer chromatography (TLC), gel permeation chromatography (GPC), sequential chromatographic analysis (SCA), paper electrophoresis and micropore filtration. Labeling was found to take place in all ranges of molecular weight. Due to the complex humic acid composition and the formation of hydroxo species the labeling yields strongly depend on the separation conditions, ranging from 42% to 80%. The pH-dependent distribution of mobile and immobile species was determined by SCA for HTcO

Kinetic Studies on the Curing Reactions of Fluoroepoxy Oligomer and Cycloaliphatic Amine

2013 ◽  
Vol 747 ◽  
pp. 753-756 ◽  
Author(s):  
Thitinun Chongtum ◽  
Wunpen Chonkaew
Keyword(s):  
Average Molecular Weight ◽  
Gel Permeation Chromatography ◽  
Kinetic Studies ◽  
Equivalent Weight ◽  
Number Average Molecular Weight ◽  
Polymeric Systems ◽  
Acid Titration ◽  
Curing Kinetic ◽  
Gel Permeation

The curing kinetic analysis is an important technique for the characterization of the curing behavior of reactive polymeric systems. In this study, fluoroepoxy oligomer was synthesized from trifluoromethyl aniline and epichlorohydrin. The epoxide equivalent weight (EEW) and the number average molecular weight (Mn) of the systhesized fluroepoxy oligomer determined from acid titration and gel permeation chromatography were found to be 312.16 g/eq and 534 g/mol, respectively. The mixtures of the fluoroepoxy oligomer were mixed with the cycloaliphatic amine in various stiochiometric ratios (1:1, 1: 1.5 and 1:2). The effects of the stiochiometric ratio on the curing behaviors were studied using both isothermal and non-isothermal DSC methods. Ozawas, Kissingers and Friedmans methods were employed to investigate the kinetic parameters. The results showed that the peak temperature (Tp) increased with the increasing heating rate. The activation energy (Ea) calculated from Ozawas and Kissingers methods were much larger than that from Friedmans method.

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