Synthesis and chromatographic characterization of [Tc-99m]technetium-humic acid species

2000 ◽  
Vol 88 (2) ◽  
Author(s):  
D. Rößler ◽  
K. Franke ◽  
R. Süß ◽  
E. Becker ◽  
H. Kupsch
Keyword(s):  
Humic Acid ◽  
Stannous Chloride ◽  
Gel Permeation Chromatography ◽  
Paper Electrophoresis ◽  
Gel Permeation ◽  
Soil Humic Acid ◽  
Chromatographic Characterization ◽  
Ph Dependent ◽  
Layer Chromatography

A natural moor soil humic acid (HA) was labeled with Tc-99m via reduction of pertechnetate with stannous chloride. The humic acid species obtained were characterized by thin layer chromatography (TLC), gel permeation chromatography (GPC), sequential chromatographic analysis (SCA), paper electrophoresis and micropore filtration. Labeling was found to take place in all ranges of molecular weight. Due to the complex humic acid composition and the formation of hydroxo species the labeling yields strongly depend on the separation conditions, ranging from 42% to 80%. The pH-dependent distribution of mobile and immobile species was determined by SCA for HTcO

scholarly journals Chromatographic characterization of products isolated from chrome shavings

1999 ◽  
Vol 42 (3) ◽  
pp. 281-290
Author(s):  
Edivaldo E Garcia ◽  
Cláudia C.M Kimura ◽  
Ariovaldo C Martins ◽  
Gisele O Rocha ◽  
Jorge Nozaki
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Chemical Characterization ◽  
Gel Permeation Chromatography ◽  
Gel Permeation ◽  
Chromatographic Characterization ◽  
Free Aminoacids

Gel permeation chromatography and high performance liquid chromatography were employed for separation and chemical characterization of products isolated from chrome shavings. After enzymatic hydrolysis, the products isolated were peptides of higher molecular weight. Peptides of lower molecular weight and free aminoacids were the main products using sulfuric acid in chrome shavings solubilization. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

scholarly journals Molar mass determination of lignins and characterization of their polymeric structures by multi-detector gel permeation chromatography

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evamaria C. Gaugler ◽  
Wolfgang Radke ◽  
Andrew P. Vogt ◽  
Dawn A. Smith
Keyword(s):  
Molar Mass ◽  
Lithium Bromide ◽  
Gel Permeation Chromatography ◽  
Mass Determination ◽  
Organosolv Lignin ◽  
Gel Permeation ◽  
Softwood Kraft Lignin ◽  
Polymeric Structures

AbstractMolar masses, Mark-Houwink-Sakurada (MHS) exponents, and refractive index increments (dn/dc) for three lignins were determined without derivatization by multi-detector gel permeation chromatography (GPC) in dimethylformamide (DMF) with 0.05 M lithium bromide (LiBr). The lack of effectiveness of fluorescence filters on molar mass determination by GPC-multi-angle laser light scattering (MALS) was confirmed for softwood kraft lignin (Indulin AT) and revealed for mixed hardwood organosolv lignin (Alcell) as well as soda straw/grass lignin (Protobind 1000). GPC with viscometry detection confirmed that these lignins were present as compact molecules. The MHS exponent α for Indulin AT and Alcell was in the order of 0.1. Additionally, the intrinsic viscosity of Protobind 1000 for a given molar mass was much lower than that of either Alcell or Indulin AT. This is the first report of dn/dc values for these three lignins in DMF with 0.05 M LiBr.

scholarly journals Molecular characterization of an end-residue of humeomics applied to a soil humic acid

RSC Advances ◽  
2014 ◽  
Vol 4 (45) ◽  
pp. 23658-23665 ◽  
Author(s):  
A. Nebbioso ◽  
A. Piccolo ◽  
M. Lamshöft ◽  
M. Spiteller
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Molecular Characterization ◽  
Natural Organic Matter ◽  
Molecular Composition ◽  
Chemical Fractionation ◽  
Soil Humic Acid

Humeomics encompasses step-wise chemical fractionation and instrumental determination to fully characterize the heterogeneous molecular composition of natural organic matter.

Kinetic Studies on the Curing Reactions of Fluoroepoxy Oligomer and Cycloaliphatic Amine

2013 ◽  
Vol 747 ◽  
pp. 753-756 ◽  
Author(s):  
Thitinun Chongtum ◽  
Wunpen Chonkaew
Keyword(s):  
Average Molecular Weight ◽  
Gel Permeation Chromatography ◽  
Kinetic Studies ◽  
Equivalent Weight ◽  
Number Average Molecular Weight ◽  
Polymeric Systems ◽  
Acid Titration ◽  
Curing Kinetic ◽  
Gel Permeation

The curing kinetic analysis is an important technique for the characterization of the curing behavior of reactive polymeric systems. In this study, fluoroepoxy oligomer was synthesized from trifluoromethyl aniline and epichlorohydrin. The epoxide equivalent weight (EEW) and the number average molecular weight (Mn) of the systhesized fluroepoxy oligomer determined from acid titration and gel permeation chromatography were found to be 312.16 g/eq and 534 g/mol, respectively. The mixtures of the fluoroepoxy oligomer were mixed with the cycloaliphatic amine in various stiochiometric ratios (1:1, 1: 1.5 and 1:2). The effects of the stiochiometric ratio on the curing behaviors were studied using both isothermal and non-isothermal DSC methods. Ozawas, Kissingers and Friedmans methods were employed to investigate the kinetic parameters. The results showed that the peak temperature (Tp) increased with the increasing heating rate. The activation energy (Ea) calculated from Ozawas and Kissingers methods were much larger than that from Friedmans method.

scholarly journals Elemental and spectroscopic characterization of humic acids fractionated by gel permeation chromatography

Agronomie ◽  
2000 ◽  
Vol 20 (5) ◽  
pp. 491-504 ◽  
Author(s):  
Aaziz Ouatmane ◽  
Valeria Dorazio ◽  
Mohamed Hafidi ◽  
Jean-Claude Revel ◽  
Nicola Senesi
Keyword(s):  
Humic Acids ◽  
Gel Permeation Chromatography ◽  
Spectroscopic Characterization ◽  
Gel Permeation

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

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