Use of latex particles for analysis of heterokaryon formation and cell fusion

1978 ◽  
Vol 4 (4) ◽  
pp. 507-512 ◽  
Author(s):  
Maura R. Levine ◽  
Rody P. Cox
Keyword(s):  
Cell Fusion ◽  
Latex Particles ◽  
Heterokaryon Formation

scholarly journals Molecular Mechanisms Regulating Cell Fusion and Heterokaryon Formation in Filamentous Fungi

2017 ◽  
pp. 215-229 ◽  
Author(s):  
Asen Daskalov ◽  
Jens Heller ◽  
Stephanie Herzog ◽  
André Fleiβner ◽  
N. Louise Glass
Keyword(s):  
Filamentous Fungi ◽  
Cell Fusion ◽  
Molecular Mechanisms ◽  
Heterokaryon Formation

scholarly journals Purkinje cell fusion and binucleate heterokaryon formation in multiple sclerosis cerebellum

Brain ◽  
2012 ◽  
Vol 135 (10) ◽  
pp. 2962-2972 ◽  
Author(s):  
K. Kemp ◽  
E. Gray ◽  
A. Wilkins ◽  
N. Scolding
Keyword(s):  
Multiple Sclerosis ◽  
Purkinje Cell ◽  
Cell Fusion ◽  
Heterokaryon Formation

A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 314-315
Author(s):  
Earl R. Walter ◽  
Glen H. Bryant
Keyword(s):  
Sample Preparation ◽  
High Vacuum ◽  
Vacuum System ◽  
Latex Particles ◽  
New Methods ◽  
Diffusion Pump ◽  
Film Forming ◽  
Routine Basis ◽  
Distribution Studies ◽  
Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

1976 ◽  
Vol 35 (02) ◽  
pp. 350-357 ◽  
Author(s):  
Hana Bessler ◽  
Galila Agam ◽  
Meir Djaldetti
Keyword(s):  
Protein Synthesis ◽  
Fold Increase ◽  
Human Platelets ◽  
Polystyrene Latex ◽  
Latex Particles ◽  
The Third ◽  
Platelet Protein ◽  
Dose Dependent ◽  
Phagocytotic Activity

SummaryA three-fold increase of protein synthesis by human platelets during in vitro phagocytosis of polystyrene latex particles was detected. During the first two hours of incubation, the percentage of phagocytizing platelets and the number of latex particles per platelet increased; by the end of the third hour, the first parameter remained stable, while the number of latex particles per cell had decreased.Vincristine (20 μg/ml of cell suspension) inhibited platelet protein synthesis. This effect was both time- and dose-dependent. The drug also caused a decrease in the number of phagocytizing cells, as well as in their phagocytotic activity.

Cell Fusion is a Potent Inducer of Aneuploidy and Drug Resistance in Tumor Cell/ Normal Cell Hybrids

2013 ◽  
Vol 18 (1 - 2) ◽  
pp. 97-113 ◽  
Author(s):  
Benjamin Berndt ◽  
Kurt S. Zanker ◽  
Thomas Dittmar
Keyword(s):  
Drug Resistance ◽  
Tumor Cell ◽  
Cell Fusion ◽  
Normal Cell ◽  
Potent Inducer ◽  
Cell Hybrids
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