scholarly journals The effects of zinc on the morphology of sickle red blood cell ghosts as observed by scanning electron microscopy

1977 ◽  
Vol 170 (2) ◽  
pp. 149-159 ◽  
Author(s):  
W. C. Kruckeberg ◽  
F. J. Oelshlegel ◽  
S. H. Shore ◽  
P. E. Smouse ◽  
G. J. Brewer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Scanning Electron

Three-dimensional analysis of morphological changes in the malaria parasite infected red blood cell by serial block-face scanning electron microscopy

2016 ◽  
Vol 193 (3) ◽  
pp. 162-171 ◽  
Author(s):  
Miako Sakaguchi ◽  
Naoyuki Miyazaki ◽  
Hisashi Fujioka ◽  
Osamu Kaneko ◽  
Kazuyoshi Murata
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Malaria Parasite ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Face Scanning ◽  
Block Face ◽  
Scanning Electron

Demonstration of an Internal Structure within the Red Blood Cell by Ion Etching and Scanning Electron Microscopy

Nature ◽  
1968 ◽  
Vol 220 (5167) ◽  
pp. 614-616 ◽  
Author(s):  
S. M. LEWIS ◽  
J. S. OSBORN ◽  
P. R. STUART
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Internal Structure ◽  
Red Blood Cell ◽  
Ion Etching ◽  
Scanning Electron

Scanning Electron Microscopy Reveals Two Distinct Classes of Erythroblastic Island Isolated from Adult Mammalian Bone Marrow

2016 ◽  
Vol 22 (2) ◽  
pp. 368-378 ◽  
Author(s):  
Jia Hao Yeo ◽  
Bronwyn M. McAllan ◽  
Stuart T. Fraser
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Surface Proteins ◽  
Erythroid Cell ◽  
Erythroid Progenitors ◽  
Hematological Disorders ◽  
And Function ◽  
Scanning Electron

AbstractErythroblastic islands are multicellular clusters in which a central macrophage supports the development and maturation of red blood cell (erythroid) progenitors. These clusters play crucial roles in the pathogenesis observed in animal models of hematological disorders. The precise structure and function of erythroblastic islands is poorly understood. Here, we have combined scanning electron microscopy and immuno-gold labeling of surface proteins to develop a better understanding of the ultrastructure of these multicellular clusters. The erythroid-specific surface antigen Ter-119 and the transferrin receptor CD71 exhibited distinct patterns of protein sorting during erythroid cell maturation as detected by immuno-gold labeling. During electron microscopy analysis we observed two distinct classes of erythroblastic islands. The islands varied in size and morphology, and the number and type of erythroid cells interacting with the central macrophage. Assessment of femoral marrow isolated from a cavid rodent species (guinea pig,Cavis porcellus) and a marsupial carnivore species (fat-tailed dunnarts,Sminthopsis crassicaudata) showed that while the morphology of the central macrophage varied, two different types of erythroblastic islands were consistently identifiable. Our findings suggest that these two classes of erythroblastic islands are conserved in mammalian evolution and may play distinct roles in red blood cell production.

scholarly journals Combining Scanning Electron Microscopy and Light Microscopy to Study How Blood Cell Directed-architecture Influences Nanoparticle Penetration into Clots

2020 ◽  
Vol 26 (S2) ◽  
pp. 2258-2261
Author(s):  
Cammy Truong ◽  
Courtney Cazzola ◽  
Sara Benzow ◽  
Austen Norberg ◽  
Ashlee Chramega ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Light Microscopy ◽  
Scanning Electron

Microscopy and Microanalysis of Blood in a Snake Head Fish, Channa gachua Exposed to Environmental Pollution

2016 ◽  
Vol 22 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Eva M. Pala ◽  
Sudip Dey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Analytical Methods ◽  
Analytical Approach ◽  
Erythrocyte Membranes ◽  
X Ray ◽  
Structural Abnormalities ◽  
Polluted Sites ◽  
Scanning Electron

AbstractConventional and highly sophisticated analytical methods (Cyria et al., 1989; Massar et al., 2012a) were used to analyze micro-structural and micro-analytical aspects of the blood of snake head fish, Channa gachua, exposed to municipal wastes and city garbage. Red (RBC) and white blood cell (WBC) counts and hemhemoglobin content were found to be higher in pollution affected fish as compared with control. Scanning electron microscopy revealed the occurrence of abnormal erythrocytes such as crenated cells, echinocytes, lobopodial projections, membrane internalization, spherocytes, ruptured cells, contracted cells, depression, and uneven elongation of erythrocyte membranes in fish inhabiting the polluted sites. Energy-dispersive X-ray spectroscopy (EDS) revealed the presence of silicon and lead in the RBCs of pollution affected fish. Significance of the study includes the highly sophisticated analytical approach, which revealed the aforementioned micro-structural abnormalities.

scholarly journals Evaluation of Blood Cell Attachment on Er:Yag Laser Applied Root Surface Using Scanning Electron Microscopy

2013 ◽  
Vol 10 (5) ◽  
pp. 560-566 ◽  
Author(s):  
Ali CEKICI ◽  
Ilay MADEN ◽  
Sercan YILDIZ ◽  
Tangul SAN ◽  
Gulden ISIK
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cell ◽  
Cell Attachment ◽  
Er:Yag Laser ◽  
Root Surface ◽  
Scanning Electron

scholarly journals Using Scanning Electron Microscopy and Atomic Force Microscopy to Study the Formation of Nanoparticles on Red Blood Cell Surface in Cervical Cancer Patients

2020 ◽  
Vol 10 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Sargylana Mamaeva ◽  
Irina Kononova ◽  
Michael Ruzhansky ◽  
Petr Nikiforov ◽  
Nadezhda Nikolaevа ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cervical Cancer ◽  
Scanning Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Cell Surface ◽  
Blood Cell ◽  
Cancer Patients ◽  
Force Microscopy ◽  
Atomic Force ◽  
Scanning Electron

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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