An in vitro model for the study of human parathyroid gland tissue: Single cell suspensions and monolayer cultures

1982 ◽  
Vol 181 (2) ◽  
pp. 147-154 ◽  
Author(s):  
P. K. Wagner ◽  
A. Knuth ◽  
U. Krause ◽  
H. Gabbert ◽  
Th. Schärfe ◽  
...  
Keyword(s):  
Single Cell ◽  
Parathyroid Gland ◽  
In Vitro Model ◽  
Cell Suspensions ◽  
Monolayer Cultures ◽  
Gland Tissue ◽  
Vitro Model

scholarly journals An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

2019 ◽  
pp. 1-8
Author(s):  
Jörg C. Gerlach ◽  
C. Johnen ◽  
B. Hartmann ◽  
J. Plettig ◽  
K. Bräutigam ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Skin Color ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Cell Suspensions ◽  
Epidermal Keratinocytes ◽  
Cell Counts ◽  
Skin Coloration ◽  
Vitro Model

A skin cell-spray grafting technique that enables the on-site application of freshly isolated autologous single cell suspensions was already applied in many cases on caucasian patients with low skin coloration. Our project hypothesis is that these suspensions contain keratinocytes and vital melanocytes, that are of particular interest for the treatment of patients of darker skin color. To test this, we applied an in vitro model, wherein the feasibility of i) isolating and ii) spraying of freshly isolated autologous melanocyte-keratinocyte cell suspensions was investigated. Primary human epidermal keratinocytes (HEKs) and melanocytes (MCs) were isolated from skin biopsies (n=8). Biochemical parameter, cell counts, cell morphology, growth behavior and immunofluorescence results were compared in two groups using MC cultures and co-cultures of MCs with HEKs. Case information on using the method clinically with one patient is included. The sprayed mixed cell suspensions proliferated in all groups without measurable loss of viability, and MCs exhibited a regular cell morphology in monoculture up to passage 4°. The sprayed MCs and HEKs demonstrated in vitro glucose and lactate metabolism that was comparable to the pipetted controls. In co-culture, well distributed CK14+ HEKs and NKI/beteb+ MCs could be demonstrated, which interacted in the in vitro model. The ratio of HEKs : MCs in our primary cultures were microscopically counted (n=8 each) as mean +/- SD 1,211,000 (+/- 574,343) HEK : 99,625 (+/- 59,025) MC; i.e., a ratio of approx. 12 : 1. Using the isolation method clinically for a patient with dark skin coloration after suffering severe second-degree burns shows a satisfying re-pigmentation of the resulting wound post healing. Freshly isolated spray-on melanocyte/keratinocyte suspensions provide for a considerable amount of viable HEKs and MCs. Using MCs in spray-grafting suspensions could represent a promising approach for treating severe partial-thickness burns and innovative therapy developments that also aim to address cosmetic aspects.

scholarly journals Single-Cell Profiling of an In Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation

Neuron ◽  
2017 ◽  
Vol 93 (5) ◽  
pp. 1035-1048.e5 ◽  
Author(s):  
Jennie L. Close ◽  
Zizhen Yao ◽  
Boaz P. Levi ◽  
Jeremy A. Miller ◽  
Trygve E. Bakken ◽  
...  
Keyword(s):  
Single Cell ◽  
In Vitro Model ◽  
Temporal Dynamics ◽  
Cell Type ◽  
Single Cell Profiling ◽  
Vitro Model ◽  
Interneuron Development

scholarly journals Modulation of Neural Network Activity through Single Cell Ablation: An in Vitro Model of Minimally Invasive Neurosurgery

Molecules ◽  
2016 ◽  
Vol 21 (8) ◽  
pp. 1018 ◽  
Author(s):  
Alessandro Soloperto ◽  
Marta Bisio ◽  
Gemma Palazzolo ◽  
Michela Chiappalone ◽  
Paolo Bonifazi ◽  
...  
Keyword(s):  
Neural Network ◽  
Minimally Invasive ◽  
Single Cell ◽  
In Vitro Model ◽  
Network Activity ◽  
Minimally Invasive Neurosurgery ◽  
Cell Ablation ◽  
Vitro Model

Analysis of granulosa cells by single cell PCR—establishment of an in vitro model system

2011 ◽  
Vol 90 (2) ◽  
pp. 176
Author(s):  
J. Pastuschek ◽  
S. Hoelters ◽  
S. Neubeck ◽  
J.S. Fitzgerald ◽  
E. Schleussner ◽  
...  
Keyword(s):  
Single Cell ◽  
Granulosa Cells ◽  
In Vitro Model ◽  
Model System ◽  
Single Cell Pcr ◽  
In Vitro Model System ◽  
Vitro Model

scholarly journals Single-Cell Profiling of an In Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation

Neuron ◽  
2017 ◽  
Vol 96 (4) ◽  
pp. 949 ◽  
Author(s):  
Jennie L. Close ◽  
Zizhen Yao ◽  
Boaz P. Levi ◽  
Jeremy A. Miller ◽  
Trygve E. Bakken ◽  
...  
Keyword(s):  
Single Cell ◽  
In Vitro Model ◽  
Temporal Dynamics ◽  
Cell Type ◽  
Single Cell Profiling ◽  
Vitro Model ◽  
Interneuron Development

scholarly journals Single-Cell and Neuronal Network Alterations in an In Vitro Model of Fragile X Syndrome

2019 ◽  
Vol 30 (1) ◽  
pp. 31-46 ◽  
Author(s):  
Anastasiya Moskalyuk ◽  
Sebastiaan Van De Vijver ◽  
Peter Verstraelen ◽  
Winnok H De Vos ◽  
R Frank Kooy ◽  
...  
Keyword(s):  
Single Cell ◽  
In Vitro Model ◽  
Ex Vivo ◽  
Fragile X ◽  
Network Development ◽  
Cellular Processes ◽  
Wide Range ◽  
Clinical Consequences ◽  
Vitro Model

Abstract The Fragile X mental retardation protein (FMRP) is involved in many cellular processes and it regulates synaptic and network development in neurons. Its absence is known to lead to intellectual disability, with a wide range of comorbidities including autism. Over the past decades, FMRP research focused on abnormalities both in glutamatergic and GABAergic signaling, and an altered balance between excitation and inhibition has been hypothesized to underlie the clinical consequences of absence of the protein. Using Fmrp knockout mice, we studied an in vitro model of cortical microcircuitry and observed that the loss of FMRP largely affected the electrophysiological correlates of network development and maturation but caused less alterations in single-cell phenotypes. The loss of FMRP also caused a structural increase in the number of excitatory synaptic terminals. Using a mathematical model, we demonstrated that the combination of an increased excitation and reduced inhibition describes best our experimental observations during the ex vivo formation of the network connections.

scholarly journals A New In Vitro Model to Study Cellular Responses after Thermomechanical Damage in Monolayer Cultures

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e82635 ◽  
Author(s):  
Alice Hettler ◽  
Simon Werner ◽  
Stefan Eick ◽  
Stefan Laufer ◽  
Frank Weise
Keyword(s):  
In Vitro Model ◽  
Cellular Responses ◽  
Monolayer Cultures ◽  
Vitro Model ◽  
Thermomechanical Damage

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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