The metabolic fate and time-dependent incorporation of dietary methionine into rumen bacterial proteins of 1-yr-old wethers has been studied following a single intraruminal administration of L-[rnethyl-14C]-methionine. Of the radioactivity found in the rumen liquor (RL) of sheep 2 h after dosing, an appreciable amount of the [methyl-14C] label was incorporated into methionine of the bacterial protein (15%) and only small amounts (< 1%) as cyst(e)ine. In another experiment, the metabolic fate of methionine labeled either in the sulfur atom or in the carbon or hydrogen atoms of the methyl group was studied in the ruminai, omasal, abomasal and duodenal contents of growing lambs. Two hours after oral administration of the L-[methyl-14C]-, L-[methyl-3H]-, or L-[35S]-rnethionine the portions of the radioactivity of rumen liquor located in the methionine of the rumen bacterial protein were 10.7, 6.9 and 7.3%, respectively while those in the cystine were less than 1% for the methyl-labeled methionine and 4.5% for the 35S methionine. Most of the radioactivity reaching the alimentary tract of growing lambs 2 h after dosing was distributed as labeled methionine in the TCA-insoluble fraction of the digesta in the omasum (86%) and abomasum (65%), whereas only 23% was found in this fraction in the duodenum. The relative amount of radioactivity incorporated into methionine of the bacterial protein from lambs which received L-[methyl-3H]-, or L-[35S]-methionine was appreciably less than that from those which received L-[methyl-14C]-methionine.