Metabolism of low molecular weight organic compounds by sulfate-reducing bacteria in a Delaware salt marsh

1985 ◽  
Vol 11 (4) ◽  
pp. 317-335 ◽  
Author(s):  
Howard J. Dicker ◽  
David W. Smith
Keyword(s):  
Molecular Weight ◽  
Salt Marsh ◽  
Organic Compounds ◽  
Sulfate Reducing Bacteria ◽  
Low Molecular Weight ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals Thermophilic sulfate-reducing bacteria Moorela thermoacetica Nadia-3, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region

2021 ◽  
Vol 15 (2) ◽  
pp. 35-46
Author(s):  
O. M. Сhayka ◽  
◽  
T. B. Peretyatko ◽  
A. A. Halushka ◽  
Keyword(s):  
Hydrogen Sulfide ◽  
Metal Ions ◽  
Organic Compounds ◽  
Elemental Sulfur ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Spoil Heap ◽  
Mining Region ◽  
Living Organisms ◽  
Reducing Bacteria

Introduction. Thermophilic sulfate-reducing bacteria attract attention of scientists as the potential agents of purification of wastewater polluted by sulfur and its compounds, heavy metal ions and organic compounds. These bacteria oxidize different organic substrates using metals with variable valency as electron acceptors and transform them into non-toxic or less toxic forms for living organisms. However, wastewater contains high concentrations of different toxic xenobiotics, particularly, metal ions that have negative influence on living organisms. For this reason, it is important to use resistant strains of microorganisms for the purification of wastewater. The aim of this work was to identify the thermophilic sulfur-reducing bacteria, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region, and to study their properties. Materials and Methods. Thermophilic sulfur-reducing bacteria were isolated from the samples of rock of “Nadiia” pit heap at 50 cm depth. Bacteria were cultivated in TF medium under the anaerobic conditions in anaerostates. Cell biomass was measured turbidimetrically using the photoelectric colorimeter KFK-3 (λ = 340 nm, 3 mm cuvette). Hydrogen sulfide content was measured photoelectrocolorymetrically by the production of methylene blue. Organic acids content was measured by high performance liquid chromatography. Cr(VI), Fe(III), Мn(IV) and NO3– content was measured turbidimetrically. Results. Thermophilic sulfur-reducing bacteria were isolated from the rock of “Nadiia” pit heap of Chervonohrad mining region. They were identified as Moorela thermoacetica based on the morpho-physiological and biochemical properties and on the results of phylogenetic analysis. M. thermoacetica Nadia-3 grow in the synthetic TF medium, have the shape of elongated rods, are gram-positive, endospore-forming. They form light brown colonies. Optimal growth was observed at 50–55 °C, pH 6.5–7. The bacteria utilize glucose, starch, fructose, maltose, lactose, sodium lactate, arabinose, cellulose, maltose, glycerol, fumarate, and ethanol as carbon sources. The highest sulfidogenic activity of M. thermoacetica Nadia-3 was found in media with glycerol, lactose, and glucose. M. thermoacetica Nadia-3 reduce SO42-, S2O32-, Fe(III), NO3–, Cr(VI) compounds besides elemental sulfur. They accumulate biomass at K2Cr2O7 concentrations of 0.1–1 mM. Sulfur reduction is not the main way of energy accumulation. Conclusions. Thermophilic chromium-resistant sulfur-reducing bacteria M. thermoacetica Nadia-3, that produce hydrogen sulfide during the oxidation of different organic compounds, were isolated from the rock of “Nadiia” pit heap. They reduce Fe(III), Cr(VI), NO3–, SO42-, S2O32-, besides elemental sulfur.

Molecular characterization of sulfate-reducing bacteria in a New England salt marsh

2005 ◽  
Vol 7 (8) ◽  
pp. 1175-1185 ◽  
Author(s):  
Michele Bahr ◽  
Byron C. Crump ◽  
Vanja Klepac-Ceraj ◽  
Andreas Teske ◽  
Mitchell L. Sogin ◽  
...  
Keyword(s):  
Salt Marsh ◽  
New England ◽  
Molecular Characterization ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
England Salt Marsh ◽  
Reducing Bacteria

Treatment of sulfate-rich and low pH wastewater by sulfate reducing bacteria with iron shavings in a laboratory

2013 ◽  
Vol 69 (3) ◽  
pp. 595-600 ◽  
Author(s):  
Xiaoye Liu ◽  
Wenqi Gong ◽  
Lin Liu
Keyword(s):  
Wastewater Treatment ◽  
Organic Compounds ◽  
Electron Acceptor ◽  
Sulfate Reducing Bacteria ◽  
Low Ph ◽  
Synthetic Wastewater ◽  
Acidic Environment ◽  
Terminal Electron Acceptor ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate-rich wastewater is an indirect threat to the environment especially at low pH. Sulfate reducing bacteria (SRB) could use sulfate as the terminal electron acceptor for the degradation of organic compounds and hydrogen transferring SO42− to H2S. However their acute sensitivity to acidity leads to a greatest limitation of SRB applied in such wastewater treatment. With the addition of iron shavings SRB could adapt to such an acidic environment, and 57.97, 55.05 and 14.35% of SO42− was reduced at pH 5, pH 4 and pH 3, respectively. Nevertheless it would be inhibited in too acidic an environment. The behavior of SRB after inoculation in acidic synthetic wastewater with and without iron shavings is presented, and some glutinous substances were generated in the experiments at pH 4 with SRB culture and iron shavings.

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