Growth and survival ofAzospirillum brasilense andArthrobacter giacomelloi in binary continuous culture

1986 ◽  
Vol 90 (1-3) ◽  
pp. 107-116 ◽  
Author(s):  
I. Cacciari ◽  
M. Del Gallo ◽  
S. Ippoliti ◽  
D. Lippi ◽  
T. Pietrosanti ◽  
...  
2015 ◽  
Vol 81 (19) ◽  
pp. 6850-6856 ◽  
Author(s):  
Dionysis I. Foustoukos ◽  
Ileana Pérez-Rodríguez

ABSTRACTContinuous culture under elevated pressures is an important technique for expanding the exploration of microbial growth and survival in extreme environments associated with the deep biosphere. Here we present a benchtop stirred continuous culture bioreactor capable of withstanding temperatures ranging from 25 to 120°C and pressures as high as 69 MPa. The system is configured to allow the employment of media enriched in dissolved gases, under oxic or anoxic conditions, while permitting periodic sampling of the incubated organisms with minimal physical/chemical disturbance inside the reactor. In a pilot experiment, the fermentative growth of the thermopiezophilic bacteriumMarinitoga piezophilawas investigated continuously for 382 h at 65°C and at pressures ranging from 0.1 to 40 MPa while the medium flow rate was varied from 2 to 0.025 ml/min. The enhanced growth observed at 30 and 40 MPa and 0.025 ml/min supports the pressure preferences ofM. piezophilawhen grown fermentatively. This assay successfully demonstrates the capabilities of the bioreactor for continuous culturing at a variety of dilution rates, pressures, and temperatures. We anticipate that this technology will accelerate our understanding of the physiological and metabolic status of microorganisms under temperature, pressure, and energy regimes resembling those of the Earth's piezosphere.


1986 ◽  
pp. 107-116
Author(s):  
I. Cacciari ◽  
M. Del Gallo ◽  
S. Ippoliti ◽  
D. Lippi ◽  
T. Pietrosanti ◽  
...  

2012 ◽  
Vol 2 (7) ◽  
pp. 529-530
Author(s):  
Jagtap H. S Jagtap H. S ◽  
◽  
S. S. Kulkarni S. S. Kulkarni

2017 ◽  
Vol 11 (3) ◽  
pp. 249
Author(s):  
Lisa Fajar Indriana ◽  
Yuli Afrianti ◽  
Sitti Hilyana ◽  
Muhammad Firdaus Firdaus

Teripang pasir, Holothuria scabra merupakan komoditas hasil laut yang bernilai ekonomis tinggi. Penangkapan berlebihan stok di alam mendorong berkembangnya kegiatan budidaya. Penempelan merupakan fase kritis pada larva teripang karena terjadi peralihan sifat planktonis ke bentik yang memerlukan substrat. Penelitian ini bertujuan untuk mengetahui preferensi penempelan, pertumbuhan, dan sintasan larva H. scabra fase penempelan pada substrat lamun yang berbeda. Penelitian menggunakan metode rancangan acak lengkap dengan perlakuan empat jenis daun lamun berbeda dan lima ulangan. Perlakuan terdiri atas Enhalus acoroides (L-1), Syringodium isoetifolium (L-2), Cymodocea serrulata (L-3), dan Cymodocea rotundata (L-4). Jumlah awal larva sebanyak 1.000 individu dan substrat dirangkai dengan luasan yang sama sebesar 12 cm x 17 cm untuk setiap unit penelitian. Hasil penelitian menunjukkan jenis lamun yang digunakan sebagai substrat berpengaruh secara nyata terhadap preferensi penempelan dan sintasan larva teripang pasir, namun tidak berpengaruh terhadap pertumbuhan. E. acoroides menunjukkan hasil terbaik dengan preferensi penempelan 0,26 ind. cm-2 dan sintasan 10,66%; sehingga layak digunakan sebagai substrat penempelan dalam pembenihan teripang pasir, H. scabra.Holothuria scabra larvae on different seagrass substrates. By: Lisa Fajar Indriana, Yuli Afrianti, Sitti Hilyana, and Muhammad FirdausSandfish Holothuria scabra is marine commodities with a high economic value. Overfishing of natural stocks has compelled an interest to begin aquaculture practice. Settlement is a critical phase for the planktonic larvae as they will transform to benthic form in the presence of substrate. This study aims to evaluate the settlement preferences, growth, and survival rate of H. scabra larvae settled on different seagrass leaves. The research was conducted using the Completely randomized design with four different species of seagrass leaves and five replications. The treatments consist of Enhalus acoroides (L-1), Syringodium isoetifolium (L-2), Cymodocea serrulata (L-3), and Cymodocea rotundata (L-4). Initial number of larvae was 1,000 individuals and the substrate was set with same widthof 12 cm x 17 cm for each unit. Results of the experiment indicated that settlement preference and survival rate of H. scabra larvae was significantly affected by seagrass used as substrate while no significantly differences was observed for growth of larvae. E. acoroides showed the best result with 0.26 ind. cm-2 settelement preference and 10.66% survival rate, so that suitable to be used as settlement substrate in H. scabra hatchery.


Author(s):  
Viyils Sangregorio-Soto ◽  
Claudia L. Garzon-Castro ◽  
Gianfranco Mazzanti ◽  
Manuel Figueredo ◽  
John A. Cortes-Romero

2019 ◽  
Vol 118 (8) ◽  
pp. 172-189
Author(s):  
Magreth B Tjizumaue ◽  
Krishna Govender

Due to the absence of appropriate Consumer Protection legislation in Namibia, there seems to be a lack of consumer protection, which may result in consumers being exploited. Since the literature reveals that Consumer Awareness, Consumer Protection, Service Quality and Customer Satisfaction influence Customer Loyalty, the aforementioned relationship was explored among consumers of long-term insurance products in Namibia. The researcher embedded the study in the Services Marketing and User’s Satisfaction/Dissatisfaction theory and developed a framework for creating a more aware consumer and thus contribute to the growth and survival of the long-term insurance industry in Namibia.  A survey was conducted using a questionnaire, among a judgmental sample of 407 long-term insurance consumers in Namibia and Structural Equation Modelling was used to analyse (eight) hypothesized relationships among the research constructs alluded to in the title of this study. In summary, it was ascertained that if the customers are fully aware (have sufficient knowledge of the products and their consumer rights), they will feel protected against unfair business practices. Furthermore, if they perceive having received quality services, they are more likely to be satisfied with the service provider and will therefore, remain loyal.


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