Genetic comparison of wild populations ofLepilemur septentrionalis andLepilemur dorsalis using RAPD markers

Primates ◽  
2001 ◽  
Vol 42 (3) ◽  
pp. 221-231 ◽  
Author(s):  
Berthine Ravaoarimanana ◽  
Jean-Luc Fausser ◽  
Yves Rumpler
2016 ◽  
Vol 44 (2) ◽  
pp. 431-436 ◽  
Author(s):  
Masoumeh YOUSEFIAZARKHANIAN ◽  
Ali ASGHARI ◽  
Jafar AHMADI ◽  
Behvar ASGHARI ◽  
Ali Ashraf JAFARI

The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.


2012 ◽  
Vol 4 (3) ◽  
pp. 33-37 ◽  
Author(s):  
Muthusamy RAJASEKAR ◽  
Muthusamy THANGARAJ ◽  
Thathiredypalli R. BARATHKUMAR ◽  
Jayachandran SUBBURAJ ◽  
Kaliyan MUTHAZHAGAN


Weed Science ◽  
1998 ◽  
Vol 46 (3) ◽  
pp. 318-321 ◽  
Author(s):  
Paloma Abad ◽  
Bernardo Pascual ◽  
José V. Maroto ◽  
Salvador López-Galarza ◽  
María J. Vicente ◽  
...  

Cultivated and weedy clones of yellow nutsedge were analyzed using random amplified polymorphic DNA (RAPD) markers to assess the polymorphism within the species and determine if this approach was suitable for identification of cultivar and wild populations. The RAPD markers unambiguously identified all studied clones. Nei-Li similarities were computed and used in an unweighted pair group method using arithmetic average (UPGMA) cluster analyses. Cultivated and weedy clones were clustered in two groups, but two cultivated clones were more closely related to weedy clones than to cultivated clones. The results showed a high level of genetic variability among the clones tested, particularly among the cultivated ones. Identification of yellow nutsedge cultivars and analysis of genetic diversity within and among weedy populations is possible by using only a small number of primers. In this study, seven selected primers discriminated among the 10 tested clones.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 454B-454
Author(s):  
C.L. Boehm ◽  
H.C. Harrison ◽  
G. Jung ◽  
J. Nienhuis

The magnitude of genetic differences among and the heterogeneity within cultivated and wild American ginseng populations is unknown. Variation among individual plants from 16 geographically separated, cultivated populations and 21 geographically separated, wild populations were evaluated using RAPD markers. Cultivated populations from the midwestern U.S., the southern U.S., and Canada were examined. Wild populations from the midwestern U.S., the southern U.S., and the eastern U.S. were examined. Polymorphic bands were observed for 15 RAPD primers, which resulted in 100 scored bands. Variation was found within and among populations, indicating that the selected populations are heterogeneous with respect to RAPD markers. The genetic relationships among individual genotypes were estimated using the ratio of discordant bands to total bands scored. Multidimensional scaling of the relationship matrix showed independent clusters corresponding to the geographical and cultural origins of the populations. The integrity of the clusters were confirmed using pooled chi-squares for fragment homogeneity. Average gene diversity (Hs) was calculated for each population sample, and a one-way analysis of variance showed significant differences among populations. Overall, the results demonstrate the usefulness of the RAPD procedure for evaluating genetic relationships and comparing levels of genetic diversity among populations of American ginseng genotypes.


2001 ◽  
Vol 120 (3) ◽  
pp. 193-196 ◽  
Author(s):  
A. Geraci ◽  
I. Divaret ◽  
F. M. Raimondo ◽  
A.-M. Chevre

2021 ◽  
Author(s):  
Ana-Maria Krapal ◽  
◽  
Oana Paula Popa ◽  
Voichița Gheoca ◽  
◽  
...  

Helix pomatia is one of the most well known species of land snails across Europe and it presents economical importance due to its consumption as food. The preliminary results of the genetic comparison between two populations, one of them under the pressure of exploitation, are presented here. As expected, most indices revealed a disequilibrium in the exploited population, with the exeption of the allelic pattern which was similar among the two studied populations.


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