The determination of vitamin d in margarine by high performance liquid chromatography

1980 ◽  
Vol 57 (12) ◽  
pp. 417-421 ◽  
Author(s):  
P. J. van Niekerk ◽  
S. C. C. Smit
Keyword(s):  
Vitamin D ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance

scholarly journals A simple method for determination of vitamin D and provitamin D in fish meat by high-performance liquid chromatography.

1987 ◽  
Vol 34 (2) ◽  
pp. 94-97 ◽  
Author(s):  
Hiramitsu SUZUKI ◽  
Seiichi HAYAKAWA ◽  
Shun WADA ◽  
Masayo OKUZUMI ◽  
Takeaki KIKUCHI
Keyword(s):  
Vitamin D ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Simple Method ◽  
Fish Meat

Review: Determination of Vitamin D in Dairy Products by High Performance Liquid Chromatography

2005 ◽  
Vol 11 (6) ◽  
pp. 451-462 ◽  
Author(s):  
S. Perales ◽  
A. Alegría ◽  
R. Barberá ◽  
R. Farré
Keyword(s):  
Vitamin D ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dairy Products ◽  
High Performance ◽  
Detection System ◽  
Chromatographic Determination ◽  
Sample Treatment ◽  
Cold Saponification

This work reviews the methods used for the determination of vitamin D in some dairy products (milk and infant formulas) by high performance liquid chromatography (HPLC). The low vitamin D contents and the presence of interfering compounds require sample treatment and purification of the extract. The advantages and drawbacks of hot and cold saponification, direct extraction and different types of extract purification are also discussed, taking into account the lack of vitamin D stability by heating, exposure to light and oxidation. With respect to chromatographic determination, the mode (normal or reverse phase), type of column (microcolumn or conventional) and detection system (UV, electrochemical and mass spectrometry) are overviewed, considering the vitamers to be determined.

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