DNA amplification fingerprinting using arbitrary oligonucleotide primers

Abstract Ten flowering dogwood (Cornus florida) cultivars were evaluated for phenotypic characters including resistance to spot anthracnose and dogwood canker, susceptibility to frost damage, leaf color, bract length and color, bloom number and reproductive phenology. Cultivars differed by at least three phenotypic characters except for ‘Barton’ and ‘Cloud 9’, which when compared with each other, were not significantly different at any characteristic. DNA amplification fingerprinting (DAF) using 17 octamer oligonucleotide primers could not distinguish these cultivars. Furthermore, arbitrary signatures from amplification profiles (ASAP), a technique that produces ‘fingerprints of fingerprints’, which is capable of detecting more differences than DAF, also failed to reveal any polymorphic DNA. Therefore, based on the lack of phenotypic and genetic differences between these two cultivars, we conclude that they are the same genotype.

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Nucleic Acid Scanning Techniques Distinguish Closely Related Cultivars of Poinsettia

HortScience ◽

10.21273/hortsci.34.6.1119 ◽

1999 ◽

Vol 34 (6) ◽

pp. 1119-1122 ◽

Cited By ~ 1

Author(s):

Terri Woods Starman ◽

Xiangrong Duan ◽

Shane Abbitt

Keyword(s):

Cluster Analysis ◽

Nucleic Acid ◽

Genetic Relationships ◽

Dna Amplification ◽

Euphorbia Pulcherrima ◽

Molecular Fingerprinting ◽

Oligonucleotide Primers

DNA amplification fingerprinting (DAF) was used to evaluate the genetic relationships among 11 cultivars of poinsettia (Euphorbia pulcherrima Willd.). Amplification was with 10 octamer oligonucleotide primers that generated 336 DNA bands. Thirty-one percent of the bands were polymorphic and distinguished among cultivars. Genetic relationships were evaluated by cluster analysis, and the resulting dendrogram closely agreed with published cultivar relationships. Arbitrary signatures from amplification profiles (ASAP) were further used to characterize two cultivars, `Nutcracker Red' and `Peterstar Red', that were previously found to be genetically and morphologically similar, as well as five cultivars in the “Freedom” series. The DAF products generated with arbitrary octamer primers were reamplified with mini-hairpin decamer primers in these experiments. The ASAP profiles were complex and yielded a total of 231 bands, 38% of which were polymorphic and capable of distinguishing each Freedom cultivar. Five of the eight primer combinations distinguished `Nutcracker Red' from `Peterstar Red'. Thus, closely related cultivars of poinsettia can be separated using new and improved molecular fingerprinting protocols.

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DNA Amplification Fingerprinting Using Very Short Arbitrary Oligonucleotide Primers

Nature Biotechnology ◽

10.1038/nbt0691-553 ◽

1991 ◽

Vol 9 (6) ◽

pp. 553-557 ◽

Cited By ~ 242

Author(s):

Gustavo Caetano-Anollés ◽

Bassam Brant J. ◽

Gresshoff Peter M.

Keyword(s):

Dna Amplification ◽

Oligonucleotide Primers

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309 GENOMIC ANALYSIS OF TWO FUNGI CAUSING ANTHRACNOSE OF DOGWOOD (CORNUS SPECIES) IN THE EASTERN UNITED STATES

HortScience ◽

10.21273/hortsci.29.5.474e ◽

1994 ◽

Vol 29 (5) ◽

pp. 474e-474

Author(s):

R. N. Trigiano ◽

G. Caetano-Anollés ◽

B. J. Bassam ◽

M. T. Windham

Keyword(s):

United States ◽

Genomic Analysis ◽

Dna Amplification ◽

Eastern United States ◽

Undescribed Species ◽

Species Relationships ◽

Polymorphic Loci ◽

Oligonucleotide Primers ◽

Discula Destructiva

DNA Amplification Fingerprinting (DAF) was used to characterize ten isolates of Discula destructiva Redlin and three isolates of an undescribed species of Discula, the causal organisms of dogwood (Cornus species) anthracnose. Isolates were obtained throughout the disease range in the eastern United States and DAF profiles generated with ten arbitrary oligonucleotide primers. Very few polymorphic loci (27/298) were detected between isolates of D. destructiva; whereas, a greater number were observed between and among the isolates of Discula species. Relationships among and between the two fungal groups were analyzed using PAUP and UPGMA and indicate that the genome of D. destructiva is highly conserved throughout the distribution. In contrast, isolates of Discula species exhibited greater variability. This suggests that D. destructive was recently introduced to the eastern United States.

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