Identification of DNA-binding proteins involved in regulation of expression of theStreptomyces aureofaciens whiH gene encoding a sporulation transcription factor

2001 ◽  
Vol 46 (6) ◽  
pp. 527-534 ◽  
Author(s):  
D. Homerová ◽  
J. Kormanec
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Regulation Of Expression ◽  
Gene Encoding

scholarly journals Regulation of Transcription by Hypoxia Requires a Multiprotein Complex That Includes Hypoxia-Inducible Factor 1, an Adjacent Transcription Factor, and p300/CREB Binding Protein

1998 ◽  
Vol 18 (7) ◽  
pp. 4089-4096 ◽  
Author(s):  
Benjamin L. Ebert ◽  
H. Franklin Bunn
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Dna Binding ◽  
Binding Proteins ◽  
Binding Protein ◽  
Hypoxia Inducible Factor ◽  
Dna Binding Proteins ◽  
Creb Binding Protein ◽  
Multiprotein Complex ◽  
Hypoxia Inducible Factor 1

ABSTRACT Molecular adaptation to hypoxia depends on the binding of hypoxia-inducible factor 1 (HIF-1) to cognate response elements in oxygen-regulated genes. In addition, adjacent sequences are required for hypoxia-inducible transcription. To investigate the mechanism of interaction between these cis-acting sequences, the multiprotein complex binding to the lactate dehydrogenase A (LDH-A) promoter was characterized. The involvement of HIF-1, CREB-1/ATF-1, and p300/CREB binding protein (CBP) was demonstrated by techniques documenting in vitro binding, in combination with transient transfections that test the in vivo functional importance of each protein. In both the LDH-A promoter and the erythropoietin 3′ enhancer, formation of multiprotein complexes was analyzed by using biotinylated probes encompassing functionally critical cis-acting sequences. Strong binding of p300/CBP required interactions with multiple DNA binding proteins. Thus, the necessity of transcription factor binding sites adjacent to a HIF-1 site for hypoxically inducible transcription may be due to the requirement of p300 to interact with multiple transcription factors for high-affinity binding and activation of transcription. Since it has been found to interact with a wide range of transcription factors, p300 is likely to play a similar role in other genes, mediating interactions between DNA binding proteins, thereby activating stimulus-specific and tissue-specific gene transcription.

A simple screening for mutant DNA binding proteins: application to murine transcription factor PEBP2α subunit, a founding member of the Runt domain protein family

Gene ◽  
1997 ◽  
Vol 185 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Yoshiko Akamatsu ◽  
Shin-ichi Tsukumo ◽  
Hiroshi Kagoshima ◽  
Naoya Tsurushita ◽  
Katsuya Shigesada
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Protein Family ◽  
Runt Domain ◽  
Subunit A ◽  
Founding Member ◽  
Domain Protein ◽  
Simple Screening

scholarly journals Differential display of DNA-binding proteins reveals heat-shock factor 1 as a circadian transcription factor

2008 ◽  
Vol 22 (3) ◽  
pp. 331-345 ◽  
Author(s):  
H. Reinke ◽  
C. Saini ◽  
F. Fleury-Olela ◽  
C. Dibner ◽  
I. J. Benjamin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Dna Binding ◽  
Differential Display ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1 ◽  
Circadian Transcription

scholarly journals Sequence of the bacteriophage SP01 gene coding for transcription factor 1, a viral homologue of the bacterial type II DNA-binding proteins.

1984 ◽  
Vol 81 (22) ◽  
pp. 7031-7035 ◽  
Author(s):  
J. R. Greene ◽  
S. M. Brennan ◽  
D. J. Andrew ◽  
C. C. Thompson ◽  
S. H. Richards ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Type Ii ◽  
Gene Coding ◽  
Transcription Factor 1 ◽  
Bacterial Type
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