In vitro expression of hepatitis C virus non-structure 5 antigen in the HepG2 cell line

2000 ◽  
Vol 20 (4) ◽  
pp. 277-279
Author(s):  
Ye Jin ◽  
Zeng Linglan ◽  
Yang Mulan ◽  
Luo Duande ◽  
Guo Jinsong
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cell Line ◽  
In Vitro Expression

scholarly journals Synthesis and Antiplasmodial Evaluation of 4-Carboxamido- and 4-Alkoxy-2-Trichloromethyl Quinazolines

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3929
Author(s):  
Dyhia Amrane ◽  
Armand Gellis ◽  
Sébastien Hutter ◽  
Marion Prieri ◽  
Pierre Verhaeghe ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Alkoxy Group ◽  
Hepg2 Cell Line ◽  
Ccl3 Group ◽  
Falciparum Strain ◽  
Structure Activity ◽  
Trichloromethyl Group ◽  
Sar Study

From three previously identified antiplasmodial hit compounds (A–C) and inactive series (D), all based on a 2-trichloromethylquinazoline scaffold, we conducted a structure-activity relationship (SAR) study at position four of the quinazoline ring by synthesizing 42 novel derivatives bearing either a carboxamido- or an alkoxy-group, to identify antiplasmodial compounds and to enrich the knowledge about the 2-trichloromethylquinazoline antiplasmodial pharmacophore. All compounds were evaluated in vitro for their cytotoxicity towards the HepG2 cell line and their activity against the multiresistant K1 P. falciparum strain, using doxorubicin, chloroquine and doxycycline as reference drugs. Four hit-compounds (EC50 K1 P. falciparum ≤ 2 µM and SI ≥ 20) were identified among 4-carboxamido derivatives (2, 9, 16, and 24) and two among 4-alkoxy derivatives (41 and 44). Regarding the two most potent molecules (16 and 41), five derivatives without a 2-CCl3 group were prepared, evaluated, and appeared totally inactive (EC50 > 50 µM), showing that the 2-trichloromethyl group was mandatory for the antiplasmodial activity.

scholarly journals Naturally Occurring Hepatitis C Virus Subgenomic Deletion Mutants Replicate Efficiently in Huh-7 Cells and Are trans-Packaged In Vitro To Generate Infectious Defective Particles

2009 ◽  
Vol 83 (18) ◽  
pp. 9079-9093 ◽  
Author(s):  
Laura Pacini ◽  
Rita Graziani ◽  
Linda Bartholomew ◽  
Raffaele De Francesco ◽  
Giacomo Paonessa
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Line ◽  
Genome Structure ◽  
Structural Proteins ◽  
Wild Type Virus ◽  
Deletion Mutants ◽  
Wild Type ◽  
Naturally Occurring

ABSTRACT Naturally occurring hepatitis C virus (HCV) subgenomic RNAs have been found in several HCV patients. These subgenomic deletion mutants, mostly lacking the genes encoding envelope glycoproteins, were found in both liver and serum, where their relatively high abundance suggests that they are capable of autonomous replication and can be packaged and secreted in viral particles, presumably harboring the envelope proteins from wild type virus coinfecting the same cell. We recapitulated some of these natural subgenomic deletions in the context of the isolate JFH-1 and confirmed these hypotheses in vitro. In Huh-7.5 cells, these deletion-containing genomes show robust replication and can be efficiently trans-packaged and infect naïve Huh-7.5 cells when cotransfected with the full-length wild-type J6/JFH genome. The genome structure of these natural subgenomic deletion mutants was dissected, and the maintenance of both core and NS2 regions was proven to be significant for efficient replication and trans-packaging. To further explore the requirements needed to achieve trans-complementation, we provided different combinations of structural proteins in trans. Optimal trans-complementation was obtained when fragments of the polyprotein encompassing core to p7 or E1 to NS2 were expressed. Finally, we generated a stable helper cell line, constitutively expressing the structural proteins from core to p7, which efficiently supports trans-complementation of a subgenomic deletion encompassing amino acids 284 to 732. This cell line can produce and be infected by defective particles, thus representing a powerful tool to investigate the life cycle and relevance of natural HCV subgenomic deletion mutants in vivo.

Evaluation of in vitro antioxidant activities of soyasaponins from soy hypocotyls in human HepG2 cell line

2016 ◽  
Vol 71 (3) ◽  
pp. 653-660 ◽  
Author(s):  
Lijie Zhu ◽  
Minghan Zhang ◽  
Xiuying Liu ◽  
He Liu ◽  
Yutang He ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Antioxidant Activities ◽  
Hepg2 Cell Line

Development of in vitro 3D cell model from hepatocellular carcinoma (HepG2) cell line and its application for genotoxicity testing

2019 ◽  
Vol 93 (11) ◽  
pp. 3321-3333 ◽  
Author(s):  
Martina Štampar ◽  
Jana Tomc ◽  
Metka Filipič ◽  
Bojana Žegura
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Cell Model ◽  
Hepg2 Cell Line ◽  
Genotoxicity Testing

scholarly journals Enhancement of the Replication of Hepatitis C Virus Replicons of Genotypes 1 to 4 by Manipulation of CpG and UpA Dinucleotide Frequencies and Use of Cell Lines Expressing SECL14L2 for Antiviral Resistance Testing

2016 ◽  
Vol 60 (5) ◽  
pp. 2981-2992 ◽  
Author(s):  
Jeroen Witteveldt ◽  
Marion Martin-Gans ◽  
Peter Simmonds
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Line ◽  
Transient Transfection ◽  
Resistance Testing ◽  
Luciferase Gene ◽  
Transient Transfection Assay ◽  
Wide Range ◽  
Frequency Changes

ABSTRACTTreatment for hepatitis C virus (HCV) has improved greatly through the use of direct-acting antivirals (DAAs). However, their effectiveness and potential for drug resistance development in non-genotype 1 variants of HCV remain relatively unexplored, asin vitroassays to assess drug susceptibility are poorly developed and unsuited for a transient-transfection format. In the current study, we have evaluated the effects of dinucleotide frequency changes in the replicon and the use of a SEC14L2-expressing cell line on the replication of HCVs of different genotypes and evaluated the resulting assay formats for measurements of susceptibility to the DAA sofosbuvir. Removal of CpG and UpA dinucleotides from the luciferase gene used in HCV replicons of genotype 1b (Con1) and genotype 2a (JFH-1) achieved between 10- and 100-fold enhancement of replication over that of the wild type posttransfection. Removal of CpG and UpA dinucleotides in the neomycin gene or deletion of the whole gene in replicons of genotype 3a (S52) and genotype 4a (ED43) enhanced replication, but phenotypic effects on altering luciferase gene composition were minimal. A further 10-fold replication enhancement of replicons from all four genotypes was achieved by using a transgenic Huh7.5 cell line expressing SECL14L2, whose expression showed a dose-dependent effect on HCV replication that was reversible by small interfering RNA (siRNA) knockdown of gene expression. By combining these strategies, the 100- to 1,000-fold enhancement of replication allowed the susceptibility of all four genotypes to the RNA polymerase inhibitor sofosbuvir to be robustly determined in a transient-transfection assay format. These methods of replication enhancement provide new tools for monitoring the susceptibility and resistance of a wide range of HCV genotypes to DAAs.

scholarly journals Hepatitis C virus infection of human hepatoma cell line 7721 in vitro

2001 ◽  
Vol 7 (5) ◽  
pp. 685 ◽  
Author(s):  
Zhi-Qiang Song ◽  
Fei Hao ◽  
Feng Min ◽  
Qiao-Yu Ma ◽  
Guo-Dong Liu
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Hepatitis C Virus Infection ◽  
Human Hepatoma Cell

scholarly journals Evaluation of Antioxidant and Antidiabetic Potential of Polyherbal Drug Made with Three Herbs: An In vitro Cell Culture Model

2021 ◽  
Vol 14 (4) ◽  
pp. 1627-1635
Author(s):  
P. Chandrasekaran
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Protein Glycation ◽  
Gas Chromatography Mass Spectrometry ◽  
Individual Plant ◽  
Hepg2 Cell Line

In diabetes, the postprandial phase is characterized by a rapid and large increase in blood glucose levels, and the possibility that the postprandial “hyperglycemic spikes” may be relevant to the onset of cardiovascular complications has recently received much attention. Medicinal use of herbal medicine in the treatment and prevention of diseases including diabetes has a long history compared to conventional medicine. These plants have no side effects and many existing medicines are derived from the plants. Hence, the current investigation was planned to make a poly herbal drug (PHD) through Punica granatum (fruits), Illicium verum (flowers) and Nyctanthes arbor (leaves) and assess their antioxidant and antidiabetic activities in vitro and in HepG2 cell line. The respective plant methanolic extracts and PHD are exposed to phytochemical assessment and to discriminate the bioactive factors by Gas Chromatography–Mass Spectrometry. We evaluated the antioxidant properties 2, 2-diphenyl-1-picrylhydrazyl scavenging, hydrogen peroxide scavenging, thiobarbituric acid reactive substances and total antioxidant activity of individual plant extracts and the PHD. At the same time, In vitro and cell culture approaches were used to assess the anti-diabetic activity. The PHD has a higher concentration of secondary metabolites than individual plant extracts, according to our findings. On the other hand, we also notice that PHD demonstrated higher antioxidant capability and considerable in vitro glucose-lowering effects along with noteworthy inhibition of ɑ-amylase, glucosidase, lipase, dipeptidyl peptidase-IV, collagenase and protein glycation in HepG2 cell line. In conclusion, this study clearly demonstrated the significant antioxidant and antidiabetic activities of the PHD. Hence, PHD may be used as a potential source in the management of diabetes, hyperglycemia and the related state of oxidative stress.

Synthesis, Characterization and Anti-hepatoma Activity of New Hederagenin Derivatives

2020 ◽  
Vol 20 (3) ◽  
pp. 252-257
Author(s):  
Xiao Liu ◽  
Lu Sun ◽  
Qing-Hua Liu ◽  
Bao-Quan Chen ◽  
Yu-Ming Liu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Normal Cell ◽  
Biological Significance ◽  
Cancer Cell Line ◽  
Human Hepatocellular Carcinoma ◽  
Hepg2 Cell Line ◽  
Normal Cell Line

Background: Based on the biological significance of hederagenin-type saponins found in our previous investigation, a series of new hederagenin derivatives were designed and synthesized. Methods : Their in vitro antiproliferative activities were evaluated against the HepG2 liver cancer cell line and normal cell line L929 by MTT assay. Results: The preliminary bioassay results demonstrated that all the tested compounds 1-7 showed potent anti-hepatoma activities, and some compounds exhibited better effects than 5-fluorouracil against human hepatocellular carcinoma HepG2 cell line. Furthermore, compound 5 showed a significant antihepatoma activity against HepG2 cells with an IC50 value of 1.88 µM. Besides, all of the tested compounds showed a low cytotoxic effect against the normal cell line L929. Conclusion: All the compounds 1-7 displayed superior selectivity against human hepatocellular carcinoma HepG2 cell line, and the results suggest that the structural modifications of C ring on the hederagenin backbone are vital for modulating anti-hepatoma activities.

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