Kinetics of enzymatic hydrolysis of lignocellulosic materials based on surface area of cellulose accessible to enzyme and enzyme adsorption on lignin and cellulose

1990 ◽  
Vol 24-25 (1) ◽  
pp. 67-73 ◽  
Author(s):  
A. O. Converse ◽  
H. Ooshima ◽  
D. S. Burns
Keyword(s):  
Enzymatic Hydrolysis ◽  
Surface Area ◽  
Lignocellulosic Materials ◽  
Enzyme Adsorption ◽  
Kinetics Of ◽  
Hydrolysis Of

Kinetics of the enzymatic hydrolysis of lignocellulosic materials at different concentrations of the substrate

2016 ◽  
Vol 8 (1) ◽  
pp. 81-87 ◽  
Author(s):  
V. V. Budaeva ◽  
E. A. Skiba ◽  
O. V. Baibakova ◽  
E. I. Makarova ◽  
S. E. Orlov ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Materials ◽  
Kinetics Of ◽  
Hydrolysis Of

scholarly journals Kinetics of Enzymatic Hydrolysis of Lignocellulosic Materials at Different Concentrations of Substrat

2015 ◽  
Vol 15 (5) ◽  
pp. 60-66 ◽  
Author(s):  
V.V. Budaeva ◽  
E.A. Skiba ◽  
O.V. Baybakova ◽  
E.I. Makarova ◽  
S.E. Orlov ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Materials ◽  
Kinetics Of ◽  
Hydrolysis Of

The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials

2016 ◽  
Vol 199 ◽  
pp. 49-58 ◽  
Author(s):  
Shaoni Sun ◽  
Shaolong Sun ◽  
Xuefei Cao ◽  
Runcang Sun
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Materials ◽  
Hydrolysis Of

scholarly journals Kinetics of Enzymatic Hydrolysis of Southeast Sulawesi Sago Starch

2020 ◽  
pp. 53-61
Author(s):  
Ansharullah Ansharullah ◽  
Muhammad Natsir
Keyword(s):  
Enzymatic Hydrolysis ◽  
Maximum Velocity ◽  
Enzyme Concentration ◽  
Hydrolysis Rate ◽  
Sago Starch ◽  
Optimum Conditions ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Substrate Concentrations ◽  
Menten Constant

The aims of this study were to characterize the kinetics of enzymatic hydrolysis of sago starch, obtained from Southeast Sulawesi Indonesia. The enzyme used for hydrolysis was bacterial ∝-amylase (Termamyl 120L from Bacillus licheniformis, E. C. 3.2.1.1).  The method to determine the initial velocity (Vo) of the hydrolysis was developed by differentiation a nonlinear equation (NLE).  The Vo of the hydrolysis was measured at various pH (6.0, 6.5,and 7.0), temperatures (40, 60, 75 and 95oC), enzyme concentrations (0.5, 1.0, 1.5 and 2.0 µg per mL) and in the presence of 70 ppm Ca++. The optimum conditions of this experiment were found to be at pH 6.5 – 7.0 and 75oC, and the Vo increased with increasing enzyme concentration. The Vo values at various substrate concentrations were also determined, which were then used to calculate the enzymes kinetics constant of the hydrolysis, including Michaelis-Menten constant (Km) and maximum velocity (Vmax) using a Hanes plot.  Km and Vmax values were found to be higher in the measurement at pH 7.0 and 75oC. The Km values  at four  different combinations of pH and temperatures (pH 6.5, 40oC; pH 6.5, 75oC; pH 7.0, 40oC; pH 7.0, 75oC) were found to be 0.86, 3.23, 0.77 and 3.83 mg/mL, respectively; and Vmax values were 17.5, 54.3, 20.3 and 57.1 µg/mL/min, respectively. The results obtained showed that hydrolysis rate of this starch was somewhat low.

scholarly journals Kinetics of enzymatic hydrolysis of polysaccharide-rich particulates

2007 ◽  
Vol 38 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Pin-Jing He ◽  
Fan Lü ◽  
Li-Ming Shao ◽  
Xiu-Jiang Pan ◽  
Duu-Jong Lee
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of

scholarly journals Effect of a Nonionic Surfactant on Enzymatic Hydrolysis of Lignocellulose Based on Lignocellulosic Features and Enzyme Adsorption

ACS Omega ◽  
2020 ◽  
Vol 5 (26) ◽  
pp. 15812-15820
Author(s):  
Wen Wang ◽  
Chaojun Wang ◽  
Zahoor ◽  
Xiaoyan Chen ◽  
Qiang Yu ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Nonionic Surfactant ◽  
Enzyme Adsorption ◽  
Hydrolysis Of
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