Effect of Particle Size on the Kinetics of Enzymatic Hydrolysis of Microcrystalline Cotton Cellulose: a Modeling and Simulation Study

2018 ◽  
Vol 187 (3) ◽  
pp. 800-816 ◽  
Author(s):  
Ashwin Gaikwad
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Modeling And Simulation ◽  
Simulation Study ◽  
Cotton Cellulose ◽  
Effect Of Particle Size ◽  
Kinetics Of ◽  
Hydrolysis Of

scholarly journals Impact of Particle Size on the Rheological Properties and Amylolysis Kinetics of Ungelatinized Cassava Flour Suspensions

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 989
Author(s):  
Tien Cuong Nguyen ◽  
Luc Fillaudeau ◽  
Dominique Anne-Archard ◽  
Son Chu-Ky ◽  
Hong Nga Luong ◽  
...  
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Biochemical Parameters ◽  
Mechanical Treatment ◽  
Particle Sizes ◽  
Cassava Flour ◽  
Effect Of Particle Size ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Newtonian Behavior

The effect of particle size on enzymatic hydrolysis of cassava flour at subgelatinization temperature was investigated. A multiscale physical metrology was developed to study the evolution of different physical-biochemical parameters: rheology, granulometry, and biochemistry. In this study, four fractions of cassava flour based on the particle sizes under 75 µm (CR075), 75–125 µm (CR125), 125–250 µm (CR250), and 250–500 µm (CR500) were screened for enzymatic hydrolysis effect. The results showed that all cassava flour suspensions exhibited a shear-thinning behavior, and the viscosity increased drastically with the increase of particle size. During hydrolysis, the viscosity reduced slightly and the non-Newtonian behavior became negligible beyond 4h of the process. The particles size for CR075 and CR125 increased steadily in diameter mean. The samples of CR250 and CR500 showed more fluctuation by first decreasing, followed by increasing in particle sizes during the process. The highest hydrolysis yield was found for samples with particle size under 125 µm (89.5–90.7%), suggesting that mechanical treatment of cassava can enhance the bioconversion rate.

Effect of particle size on the enzymatic hydrolysis of polysaccharides from ultrafine lignocellulose particles

2012 ◽  
Vol 48 (3) ◽  
pp. 312-317 ◽  
Author(s):  
V. V. Shutova ◽  
A. I. Yusipovich ◽  
E. Yu. Parshina ◽  
D. O. Zakharkin ◽  
V. V. Revin
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Effect Of Particle Size ◽  
Hydrolysis Of

Effect of particle size on enzymatic hydrolysis of pretreated Miscanthus

2013 ◽  
Vol 44 ◽  
pp. 11-17 ◽  
Author(s):  
Esha Khullar ◽  
Bruce S. Dien ◽  
Kent D. Rausch ◽  
M.E. Tumbleson ◽  
Vijay Singh
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Effect Of Particle Size ◽  
Hydrolysis Of

scholarly journals Kinetics of Enzymatic Hydrolysis of Southeast Sulawesi Sago Starch

2020 ◽  
pp. 53-61
Author(s):  
Ansharullah Ansharullah ◽  
Muhammad Natsir
Keyword(s):  
Enzymatic Hydrolysis ◽  
Maximum Velocity ◽  
Enzyme Concentration ◽  
Hydrolysis Rate ◽  
Sago Starch ◽  
Optimum Conditions ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Substrate Concentrations ◽  
Menten Constant

The aims of this study were to characterize the kinetics of enzymatic hydrolysis of sago starch, obtained from Southeast Sulawesi Indonesia. The enzyme used for hydrolysis was bacterial ∝-amylase (Termamyl 120L from Bacillus licheniformis, E. C. 3.2.1.1).  The method to determine the initial velocity (Vo) of the hydrolysis was developed by differentiation a nonlinear equation (NLE).  The Vo of the hydrolysis was measured at various pH (6.0, 6.5,and 7.0), temperatures (40, 60, 75 and 95oC), enzyme concentrations (0.5, 1.0, 1.5 and 2.0 µg per mL) and in the presence of 70 ppm Ca++. The optimum conditions of this experiment were found to be at pH 6.5 – 7.0 and 75oC, and the Vo increased with increasing enzyme concentration. The Vo values at various substrate concentrations were also determined, which were then used to calculate the enzymes kinetics constant of the hydrolysis, including Michaelis-Menten constant (Km) and maximum velocity (Vmax) using a Hanes plot.  Km and Vmax values were found to be higher in the measurement at pH 7.0 and 75oC. The Km values  at four  different combinations of pH and temperatures (pH 6.5, 40oC; pH 6.5, 75oC; pH 7.0, 40oC; pH 7.0, 75oC) were found to be 0.86, 3.23, 0.77 and 3.83 mg/mL, respectively; and Vmax values were 17.5, 54.3, 20.3 and 57.1 µg/mL/min, respectively. The results obtained showed that hydrolysis rate of this starch was somewhat low.

scholarly journals Kinetics of enzymatic hydrolysis of polysaccharide-rich particulates

2007 ◽  
Vol 38 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Pin-Jing He ◽  
Fan Lü ◽  
Li-Ming Shao ◽  
Xiu-Jiang Pan ◽  
Duu-Jong Lee
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of
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