Gene expression of the androgen repressed rat TR2 orphan receptor: a member of steroid receptor superfamily

Endocrine ◽  
1995 ◽  
Vol 3 (4) ◽  
pp. 277-283 ◽  
Author(s):  
Ritsuro Ideta ◽  
Shuyuan Yeh ◽  
Yifen Lee ◽  
Kenji Adachi ◽  
Hiroyuki Takeda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Steroid Receptor ◽  
Orphan Receptor

scholarly journals Suppression of the Human Erythropoietin Gene Expression by the TR2 Orphan Receptor, a Member of the Steroid Receptor Superfamily

1996 ◽  
Vol 271 (17) ◽  
pp. 10405-10412 ◽  
Author(s):  
Han-Jung Lee ◽  
Win-Jing Young ◽  
Charles C.-Y. Shih ◽  
Chawnshang Chang
Keyword(s):  
Gene Expression ◽  
Steroid Receptor ◽  
Orphan Receptor ◽  
Human Erythropoietin

The ARP-1 orphan receptor represses steroid-mediated stimulation of human placental lactogen gene expression

1996 ◽  
Vol 16 (3) ◽  
pp. 221-227 ◽  
Author(s):  
A Stephanou ◽  
M Shah ◽  
B Richardson ◽  
S Handwerger
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Expression Vector ◽  
Steroid Receptor ◽  
Orphan Receptor ◽  
Placental Lactogen ◽  
Expression Vectors ◽  
Human Placental Lactogen ◽  
Mobility Shift ◽  
Stimulation Of

ABSTRACT Studies were performed to determine whether ARP-1, which is an orphan receptor of the steroid receptor superfamily, inhibits basal activity of the human placental lactogen (hPL) promoter and the increase in hPL promoter activity in response to the receptors for thyroid hormone (TR) and retinoic acid (RAR). Co-transfection of an ARP-1 expression vector into BeWo choriocarcinoma cells, along with an expression vector containing 1·2 kb of the hPL promoter coupled to a CAT reporter gene, resulted in a dose-dependent inhibition of basal CAT activity. In addition, ARP-1 inhibited the stimulation of CAT activity by RARα and TRβ expression vectors. Mobility shift assays demonstrated that ARP-1 binds specifically to a composite steroid response element on the hPL promoter that confers retinoic acid and T3 responsiveness. The results implicate an inhibitory role for ARP-1 in the regulation of hPL gene expression and strongly suggest that hPL gene expression is regulated, at least in part, by the interaction of stimulatory and inhibitory members of the steroid receptor superfamily.

The Effect of Vitamin A Supplementation on Retinoic Acid-Related Orphan Receptor γt (RORγt) and Interleukin-17 (IL-17) Gene Expression in Avonex-Treated Multiple Sclerotic Patients

2013 ◽  
Vol 51 (3) ◽  
pp. 749-753 ◽  
Author(s):  
Niyaz Mohammadzadeh Honarvar ◽  
Mohammad Hossein Harirchian ◽  
Fariba Koohdani ◽  
Feridoun Siassi ◽  
Mina Abdolahi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Vitamin A ◽  
Orphan Receptor ◽  
Interleukin 17 ◽  
Vitamin A Supplementation

Gene expression of NOR-1, a neuron-derived orphan receptor, is inducible in neuronal and other cell lineages in culture

1995 ◽  
Vol 115 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Shuji Bandoh ◽  
Toshihiko Tsukada ◽  
Kouji Maruyama ◽  
Naganari Ohkura ◽  
Ken Yamaguchi
Keyword(s):  
Gene Expression ◽  
Orphan Receptor ◽  
Cell Lineages

Steroid receptor-mediated inhibition of rat prolactin gene expression does not require the receptor DNA-binding domain

Cell ◽  
1988 ◽  
Vol 52 (5) ◽  
pp. 685-695 ◽  
Author(s):  
Stuart Adler ◽  
Marian L. Waterman ◽  
Xi He ◽  
Michael G. Rosenfeld
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Steroid Receptor ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Prolactin Gene

Vitamin B6 Modulation of Steroid Receptor-mediated Gene Expression

1994 ◽  
pp. 319-327 ◽  
Author(s):  
Douglas B. Tully ◽  
Alyson B. Scoltock ◽  
John A. Cidlowski
Keyword(s):  
Gene Expression ◽  
Vitamin B6 ◽  
Steroid Receptor

scholarly journals A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile

2020 ◽  
Vol 34 ◽  
pp. 205873842090718
Author(s):  
LA Bautista-Herrera ◽  
U De la Cruz-Mosso ◽  
IV Román-Fernández ◽  
I Parra-Rojas ◽  
JG Soñanez-Organis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Psoriatic Arthritis ◽  
Mrna Expression ◽  
Close Association ◽  
Quantitative Polymerase Chain Reaction ◽  
Serum Levels ◽  
Cytokine Profile ◽  
Orphan Receptor ◽  
Cross Sectional ◽  
Th17 Cytokine

The goals of our study were to determine the possible association of interleukin (IL)-31 with Th17 cytokine profile in serum and to quantify retinoic acid-related orphan receptor C ( RORC) mRNA expression in psoriatic arthritis (PsA) patients. This cross-sectional study was conducted in 50 patients with PsA and 30 control subjects (CS) matched by age and gender. The cytokine serum levels were quantified by magnetic bead–based assay using the Bio-Plex MAGPIX system, and RORC mRNA expression was determined by quantitative polymerase chain reaction (qPCR). As a result, significant differences in IL-31 were observed between study groups (77.23 pg/mL in PsA vs 64.4 pg/mL in CS, P < 0.001) and Th17 cytokine profile serum levels (IL-17A: 6.36 pg/mL in PsA vs 2.97 pg/mL in CS, P = 0.02; IL-17F: 44.15 pg/mL in PsA vs 23.36 pg/mL in PsA, P = 0.01; IL-17E: 3.03 pg/mL in PsA vs 0.82 pg/mL in CS, P < 0.001; IL-21: 36.45 pg/mL in PsA vs 12.44 pg/mL in CS, P = 0.02); however, significant differences were not observed for IL-23 (31.2 pg/mL in PsA vs 53.26 pg/mL in CS, P = 0.58). Furthermore, positive correlations between IL-31 and Th17 cytokine profile serum levels were found (IL-17A: rs = 0.64, P < 0.001; IL-17F: rs = 0.73, P < 0.001; IL-17E: rs = 0.70, P < 0.001; IL-21: rs = 0.54, P = 0.002; IL-23: rs = 0.5, P < 0.01). Regarding RORC gene expression, the PsA group showed an increase of 6.85-fold compared to the CS group. We did not find any association between the serum levels of cytokines and RORC gene expression. In conclusion, in PsA, there are increased serum levels of IL-31, IL-17A, IL-17F, IL-17E, and IL-21, but not IL-23. Moreover, there was a positive correlation of IL-31 with the Th17 cytokine profile and a high RORC gene expression. Altogether, these findings suggest a proinflammatory contribution of IL-31 in close association with the Th17 cytokine profile in PsA.

scholarly journals Retinoic acid receptor-related orphan receptor (ROR) α4 is the predominant isoform of the nuclear receptor RORα in the liver and is up-regulated by hypoxia in HepG2 human hepatoma cells

2002 ◽  
Vol 364 (2) ◽  
pp. 449-456 ◽  
Author(s):  
Caroline CHAUVET ◽  
Brigitte BOIS-JOYEUX ◽  
Jean-Louis DANAN
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Hepg2 Cells ◽  
Transcriptional Activity ◽  
Cobalt Chloride ◽  
Retinoic Acid Receptor ◽  
Orphan Receptor ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Acid Receptor

The retinoic acid receptor-related orphan receptor α (RORα) is critically involved in many physiological functions in several organs. We find that the main RORα isoform in the mouse liver is the RORα4 isoform, in terms of both mRNA and protein levels, while the RORα1 isoform is less abundant. Because hypoxia is a major feature of liver physiology and pathology, we examined the effect of this stress on Rora gene expression and RORα transcriptional activity. HepG2 human hepatoma cells were cultured for 24h under normoxia (20% O2) or hypoxia (10, 2, and 0.1% O2) and the abundance of the Rora transcripts measured by Northern blot and semi-quantitative RT-PCR. Hypoxic HepG2 cells contained more Rora mRNA than controls. This was also observed in rat hepatocytes in primary culture. Cobalt chloride and desferrioxamine also increased the amount of Rora mRNA in HepG2 cells. It is likely that these treatments increase the amount of the RORα4 protein in HepG2 cells as evidenced by Western blotting in the case of desferrioxamine. Transient transfection experiments indicated that hypoxia, cobalt chloride, and desferrioxamine all stimulate RORα transcriptional activity in HepG2 cells. Hence, we believe that RORα participates in the control of gene transcription in hepatic cells and modulates gene expression in response to hypoxic stress.

scholarly journals Endometrial Endothelial Cell Steroid Receptor Expression and Steroid Effects on Gene Expression

2005 ◽  
Vol 90 (3) ◽  
pp. 1812-1818 ◽  
Author(s):  
Graciela Krikun ◽  
Frederick Schatz ◽  
Robert Taylor ◽  
Hilary O. D. Critchley ◽  
Peter A. W. Rogers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cell ◽  
Steroid Receptor ◽  
Receptor Expression ◽  
Steroid Receptor Expression

279. Expression of oestrogen receptor-α and modulators of steroid receptor signalling, proline-rich nuclear receptor-2 and peptidylprolyl isomerade-D, in the hypothalamus of suckled and weaned postpartum beef cows

2008 ◽  
Vol 20 (9) ◽  
pp. 79
Author(s):  
T. Flatscher-Bader ◽  
A. H. M. Suhaimi ◽  
SA Lehnert ◽  
A. Reverter ◽  
W. Barris ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nuclear Receptor ◽  
Oestrogen Receptor ◽  
Arcuate Nucleus ◽  
Gene Clusters ◽  
Steroid Receptor ◽  
Beef Cows ◽  
Hypothalamic Nucleus ◽  
Receptor Signalling ◽  
Oestrogen Receptor Α

The aim was to characterise gene expression in the hypothalamus of suckled and weaned postpartum beef cows. The hypothalamus was obtained at slaughter from 12 primiparous Brahman cows (Zebu, Bos indicus) at 27 and 34 days postpartum. Six cows were weaned 7 days or 14 days before slaughter. Hypothalamic regions used for gene expression were: H1 (SC-POA, APVN, anterior hypothalamic nucleus, anterior portion of the arcuate nucleus, nearby areas of the diagonal band of Broca, and medial septum); H2 (basal hypothalamus-median eminence, ventromedial hypothalamus, posterior portion of the arcuate nucleus, and anterior part of the mammillary body). Gene expression was determined using the Agilent bovine 44k DNA microarray and differential expression (DE) was ascertained by mixed model analysis. A total of 122 genes were DE in H1 and 84 genes were DE in H2; 41 DE genes were common to H1 and H2. Functional clustering of DE genes using DAVID (www.david.abcc.ncifcrf.gov) revealed DE gene clusters in H1 associated with signalling events and ion binding, and DE gene clusters in H2 associated with hormone activity and ligand-receptor interactions. Of the DE genes, ~25% were linked with oestrogen signalling. This included oestrogen receptor-α (ESR1) that showed lower DE in H2 for weaned cows. Two modulators of steroid receptor signalling, proline-rich nuclear receptor coactivator-2 (PNRC2)1 and peptidylprolyl isomerase D (PPID)2, showed altered expression. In weaned cows, expression level of PNRC2 was lower in H1 and H2, while that of PPID was decreased in H1. The overlapped hypothalamic regions H1 and H2 are known to contain GnRH neuron terminals and kisspeptin neurons. Weaning promotes the resumption of cyclic ovarian function in postpartum cows, and the similar shifts in DE of ESR1, PNRC2 and PPID provided further evidence of a role for oestradiol at the hypothalamus in regulating postpartum reproduction. (1) Zhou D et al. 2006 Nucleic Acids Res 34:5974–86 (2) Kumar P et al. 2001 Biochem Biophys Res Commun 284:219–25

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