In planta 2,3,5 triiodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet ( Beta vulgaris L.) plants

In genetic improvement of mungbean much success has not been achieved due to its recalcitrant nature towards in vitro regeneration. An attempt was made to develop an Agrobacterium-mediated in planta genetic transformation protocol for a locally grown mungbean variety BARI Mung-3 using a screenable marker gene. Two minutes of vacuum infiltration followed by 60 minutes of incubation period in Agrobacterium suspension of Winans’ AB medium containing wounded tobacco leaf extract was found most suitable towards genetic transformation in pricked de-coated half seed explants. An optical density (OD600) of 0.7 was found most effective for transient gus gene expression. Chimeric GUS expression was observed in the root and leaf tissues from the successfully transformed plantlets obtained through in planta transformation. This methodology of genetic transformation was found more suitable, easier and less time consuming than tissue culture based genetic transformation, which may be used for the genetic improvement of mungbean.

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High Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale - AB Genome

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v21i2.10243 ◽

2011 ◽

Vol 21 (2) ◽

pp. 199-205 ◽

Cited By ~ 2

Author(s):

K. Girish Kumar ◽

V. Krishna ◽

Venkatesh Venkatesh ◽

K. Pradeep

Keyword(s):

Callus Induction ◽

High Frequency ◽

In Vitro Regeneration ◽

Floral Part ◽

Bud Formation ◽

Shoot Bud ◽

Immature Male ◽

Floral Explants ◽

High Frequency Induction

High frequency in vitro regeneration for mass multiplication from immature male floral explants of Musa paradisica cv. Puttabale on MS supplemented with adenine sulfate (160 mg/l), tyrosine (100 mg/l), sucrose (40 g/l) and gelled with 0.8 g/l agar was attempted. For callus induction the combinations of 2, 4-D and BAP were tested at 1.0 - 10.0 mg/l and 0.5 - 5.0 mg/l, respectively. For shoot bud formation combinations of BAP and TDZ were also tested at 1.0 - 5.0 mg/l and 0.1 - 0.5 mg/l, respectively. Luxuriant proliferation and high frequency induction (97.0%) of callus was noticed from the accessory floral part of the explant at 7.0 mg/l 2, 4-D and 1.0 mg/l BAP, later it preceded towards the gynoecium. Interaction of BAP (2.0 5.0 mg/l) and TDZ (0.2 - 0.5 mg/l) would provoke high frequency shoot bud differentiation from the floral calli and a mean of 29.40 ± 6.10 shootlets per callus was obtained at 4 mg/l BAP and 0.4 mg/l TDZ. Rooting of the microshoots was achieved on MS containing 0.6 mg/l NAA and 0.2% activated charcoal. Key words: Musa pardisica, Puttabale, Regeneration, Male floral explants. D. O. I. 10.3329/ptcb.v21i2.10243 Plant Tissue Cult. & Biotech. 21(2): 199-205, 2011 (December)

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CURRENT STATUS OF TRANSGENIC COTTON-UTILITY OF GENOTYPE INDEPENDENT IN PLANTA APPROACHES FOR THE GENERATION OF TRANSGENICS

International Journal on Agricultural Sciences ◽

10.53390/ijas.v11i2.6 ◽

2020 ◽

Vol 11 (2) ◽

Author(s):

Kesiraju Karthik

Keyword(s):

Tissue Culture ◽

In Vitro Regeneration ◽

Crop Improvement ◽

Transgenic Cotton ◽

Current Status ◽

In Planta ◽

Biotic And Abiotic Stresses ◽

Independent Manner ◽

Gossypium Spp

Cotton (Gossypium spp.), is a mercantile crop plant is grown for its fluffy fiber and cotton seed oil in around 70 countries worldwide. Cotton is an economically important crop, shows erratic productivity under rain feed conditions; it is bogged down with many biotic and abiotic stresses. Due to lack of resistant germplasm, crop improvement through conventional breeding practices has been lagging. Genetic engineering offers numerous protocols to engineer plants to overcome stress. Biotechnological intervention for cotton improvement has begun three decades ago. The recalcitrance of cotton to tissue culture has been the major constraint for in vitro regeneration. Alternate methods that evade tissue culture regeneration steps have thus been envisaged. Till date there are very few standardized protocols that can be employed to develop transgenics in a genotype independent manner. Thus, genotype independent in planta transformation strategies have gained momentum in the present days, but reproducibility of reported protocols remains an amigna in many cases. In planta transformations holds prominence due to viability and ease in generation of transgenic cotton plants with in less time. This review focuses on grouping efforts made by different research groups in this senior. Several reports and standardizations have been focused that reports development of transgenic cotton.

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Mature Embryo-Based in vitro Regeneration of Indica Rice Cultivars for High Frequency Plantlets Production

Bangladesh Rice Journal ◽

10.3329/brj.v20i2.34132 ◽

2017 ◽

Vol 20 (2) ◽

pp. 81-87

Author(s):

HN Barman ◽

ME Hoque ◽

RK Roy ◽

PL Biswas ◽

MAI Khan ◽

...

Keyword(s):

Callus Induction ◽

High Frequency ◽

Indica Rice ◽

In Vitro Regeneration ◽

Mature Embryo ◽

Ms Medium ◽

Rice Varieties ◽

Growing Medium ◽

Regeneration Efficiency

The study was conducted at Biotechnology Division of Bangladesh Rice Research Institute (BRRI) to investigate the effects of plant growing medium and plant growth regulator (PGR) for the callus induction and high frequency plantlets regeneration of indica rice. Ten indica rice varieties viz. BR5, BR11, BRRI dhan28, BRRI dhan29, BRRI dhan33, BRRI dhan41, BRRI dhan47, BRRI dhan48, BRRI dhan49 and BRRI dhan50 were cultured on MS, N6 and LS media. The MS medium was found better for callus induction as compared to N6 and LS media. Among the tested varieties BRRI dhan48 induced the highest percent and best quality callus. Interaction effects of BRRI dhan48 to MS medium yielded 71.55% callus induction. The regeneration efficiency of BRRI dhan48 was tested on MS medium supplemented with different combination of NAA plus BAP and NAA plus kinetin. MS medium supplemented with 2.0 mg L-1 NAA and 2.0 mg L-1 Kn was found the best in respect of percent regenerated (76.67%) plantlet as well as for the growth of plantlets in vitro.Bangladesh Rice j. 2016, 20(2): 81-87

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High frequency in vitro regeneration of Lathyrus sativus L.

Biologia Plantarum ◽

10.1007/s10535-005-0066-x ◽

2005 ◽

Vol 49 (4) ◽

pp. 637-639 ◽

Cited By ~ 6

Author(s):

D. P. Barik ◽

U. Mohapatra ◽

P. K. Chand

Keyword(s):

High Frequency ◽

In Vitro Regeneration ◽

Lathyrus Sativus ◽

Lathyrus Sativus L

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EFFECTS OF DIFFERENT PHYSIOCHEMICAL FACTORS ON REGENERATION OF CYMBOPOGON POLYNEUROS STAPF VIA CALLUS CULTURE AND SUBSEQUENT CHROMOSOMAL STABILITY

Israel Journal of Plant Sciences ◽

10.1080/07929978.1999.10676773 ◽

1999 ◽

Vol 47 (3) ◽

pp. 195-198

Author(s):

Anath Bandhu Das

Keyword(s):

Callus Culture ◽

High Frequency ◽

Potassium Salt ◽

In Vitro Regeneration ◽

Basal Medium ◽

Naphthalene Acetic Acid ◽

Leaf Base ◽

Regenerated Plants ◽

The Individual

In vitro regeneration of Cymbopogon polyneuros Stapf was obtained through callus culture using leaf base, node, and root as explants. Callus was induced from different explants with 2–5 mg/1 α-naphthalene acetic acid (NAA) and 1–2 mg/1 kinetin in Murashige and Skoog's (MS) basal medium. High frequency shoots were noticed from leaf-base callus supplemented with 3.5 mg/1 6-benzylaminopurine (BA), L-arginine, adenine, and a low level of NAA (0.2 mg/1). About 80–85 shoot buds were obtained from ca. 200 mg of callus per culture. The individual shoots produced root in the presence of 0.5–3 mg/1 indole 3-butyric acid or its potassium salt. Regenerated plants were cytologically and phenotypically stable. Regenerants were transplanted into soil and subsequently transferred to the field.

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AN EFFICIENT IN VITRO REGENERATION METHOD TO PRODUCE ADVENTITIOUS PLANTS IN SWEETPOTATO

HortScience ◽

10.21273/hortsci.28.4.262b ◽

1993 ◽

Vol 28 (4) ◽

pp. 262B-262

Author(s):

C. S. Prakash ◽

R. Gosukonda ◽

A. Porobo Dessai ◽

E. Blay ◽

K. Dumenvo

Keyword(s):

Gene Transfer ◽

High Frequency ◽

In Vitro Regeneration ◽

Leaf Explants ◽

Zeatin Riboside ◽

Limiting Factor ◽

Ms Medium ◽

Petiole Explants ◽

Green House

Lack of suitable methods to develop adventitious plantlets in vitro is a limiting factor in producing transgenic sweetpotato plants through gene transfer. Studies were conducted to develop an in vitro high frequency regeneration protocol for sweetpotato that is rapid and consistent. When 27 genotypes of sweetpotato were screened, five were identified as highly regenerative (318846-3, PI 531143, Hi Dry, Rojoblanco and Beauregard). High frequency regeneration of shoots (in 60 to 80% explants) was observed within 30 days when leaf explants with intact petioles from the apical portions of the in vitro shoots were cultured on a MS medium with 2,4-D (0.2 mg/l) for three days and then transferred to a medium with zeatin riboside (ZR) (0.2 mg/l). However, thidiazuron (0.2 mg/l) had to be substituted for ZR to achieve regeneration of shoots from petiole (0.5 to 1 cm) explants (the most responsive organ for transformation by Agrobacterium). Petiole explants developed shoots efficiently (80-90%) and rapidly (10 to 21 d), but were specific to the genotype 318846-3. The resulting plantlets were vigorous and normal, and were transferred to the green house with little or no mortality.

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