Effect of plant growth regulators and medium composition on cell growth and saponin production during cell-suspension culture of mountain ginseng (Panax ginseng C. A. mayer)

2002 ◽  
Vol 45 (4) ◽  
pp. 201-206 ◽  
Author(s):  
Mei-Lan Lian ◽  
Debasis Chakrabarty ◽  
Kee-Yoeup Paek
Keyword(s):  
Cell Growth ◽  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Panax Ginseng ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Medium Composition

scholarly journals Influence of plant growth regulators and salicylic acid on the production of some secondary metabolites in callus and cell suspension culture of Satureja sahendica Bornm.

2021 ◽  
Vol 117 (4) ◽  
pp. 1
Author(s):  
Sarieh TARIGHOLIZADEH ◽  
Rouhollah MOTAFAKKERAZAD ◽  
Morteza KOSARI-NASAB ◽  
Ali MOVAFEGHI ◽  
Sakineh MOHAMMADI ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Secondary Metabolites ◽  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Total Phenolics ◽  
Fresh Mass

<p class="042abstractstekst">The impact of combinations of plant growth regulators (PGRs) on callus culture of <em>Satureja sahendica </em>Bornm. was investigated. In nodal explants, the response of secondary metabolite production to different concentrations of PGRs was analyzed regarding the presence and absence of polyvinylpyrrolidone (PVP). The explants were cultured on MS media in presence of auxins (2,4-dichlorophenoxyacetic acid and naphthylacetic acid) and cytokinins (thidiazuron and kinetin); which were used in equal concentrations of 0.5, 1, and 2 mg l<sup>-1</sup>. The treatment of 2 mg l<sup>-1</sup> 2,4-D + 2 mg l<sup>-1 </sup>Kin (MD3) led to the highest production of total phenolics (4.303 ± 0.449 mg GAE g<sup>-1</sup>) and flavonoids (24.903 ± 7.016 mg QE g<sup>-1</sup>). Moreover, the effect of salicylic acid (SA) on the production of secondary metabolites in cell suspension culture of <em>Satureja sahendica</em> was evaluated. The cell suspension culture was established by culturing the nodal-derived friable callus in the liquid medium containing different concentrations of SA (0, 100, 150, 200 µM). An inverse relationship exists between the fresh mass and secondary metabolites contents. In addition, there was a significant difference among concentrations of SA in the production of total phenolics and flavonoid compounds. SA enhances secondary metabolites production and decreases cell fresh mass.</p>

scholarly journals Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst.

2018 ◽  
Vol 28 (2) ◽  
pp. 191-199 ◽  
Author(s):  
AV Kharde ◽  
SV Kore ◽  
MB Khetmalas
Keyword(s):  
Plant Growth ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Bacopa Monnieri ◽  
Naphthalene Acetic Acid

Cell suspension culture is used for the optimization of plant growth regulators used for the elicitation of bacoside content of Bacopa monnieri (L.) Wettst. Callus induction was carried out on MS supplemented with 2, 4-D (0.5 mg/l) by using leaf explants. For suspension culture MS liquid medium was supplemented with constant α-naphthalene acetic acid (0.5 mg/l) and casein hydrolysate (1 g/l) as well as the varied concentrations of 6-benzyleadenine, kinetin, thidiazuaron and chitosan. It was observed that bacoside production was increased by 6- benzyleadenine (0.5 mg/l), Kn (0.5 mg/l) and thidiazuaron (0.25 mg/l) was 3.07, 3.79 and 3.97 mg/g, respectively. This indicates that lower concentration of growth regulator favor the bacoside enhancement under in vitro conditions, while chitosan affects the bacoside production in suspension culture. In this study thidiazuaron at 0.25 mg/l induces maximum elicitation of bacoside content i.e. 3.97 mg/g. Plant Tissue Cult. & Biotech. 28(2): 191-199, 2018 (December)

Growth Behavior of a Liverwort, Jungermannia subulata Evans, in a Cell Suspension Culture. The Role of Organic Acids Required for Cell Growth

1981 ◽  
Vol 22 (8) ◽  
pp. 1533-1540 ◽  
Author(s):  
Yoshimoto Ohta ◽  
Momoyo Ishikawa ◽  
Setsuko Abe ◽  
Kenji Katoh ◽  
Yoshio Hirose
Keyword(s):  
Cell Growth ◽  
Organic Acids ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Growth Behavior ◽  
A Cell

scholarly journals Handling culture medium composition for optimizing plant cell suspension culture in shake flasks

2017 ◽  
Vol 133 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Tiago Fidemann ◽  
Gabriela Aparecida de Araujo Pereira ◽  
Tárik Reis Heluy ◽  
Rodrigo Boccoli Gallego ◽  
Mônica Rosa Bertão ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Culture Medium ◽  
Medium Composition ◽  
Plant Cell Suspension Culture ◽  
Plant Cell Suspension ◽  
Shake Flasks

The effect of plant growth regulators on the production of canthin-6-one alkaloids by Brucea javanica cell suspension cultures

1994 ◽  
Vol 37 (2) ◽  
pp. 421-424 ◽  
Author(s):  
Karin C.-S. Liu ◽  
Margaret F. Roberts ◽  
B.Curt Homeyer ◽  
Yang Shi-Lin ◽  
J.David Phillipson
Keyword(s):  
Plant Growth ◽  
Cell Suspension ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Brucea Javanica

scholarly journals Enhanced production of phenolic acids in cell suspension culture of Salvia leriifolia Benth. using growth regulators and sucrose

2018 ◽  
Vol 70 (2) ◽  
pp. 741-750 ◽  
Author(s):  
Masoomeh Modarres ◽  
Sedigheh Esmaeilzadeh Bahabadi ◽  
Mohammad Ehsan Taghavizadeh Yazdi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Cell Suspension Culture ◽  
Enhanced Production
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