Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes

Production of wheat-alien disomic addition lines is of great value to the exploitation and utilization of elite genes originated from related species to wheat. In this study, a novel wheat-Aegilops biuncialis 5Mb disomic addition line WA317 was characterized by in situ hybridization (ISH) and specific-locus amplified fragment sequencing (SLAF-seq) markers. Compared to its parent Chinese Spring (CS), the glumes of WA317 had black color and were difficult to remove after harvesting, suggesting chromosome 5Mb carried gene(s) related to glume development and Triticeae domestication process. A total of 242 Ae. biuncialis SLAF-based markers (298 amplified patterns) were developed and further divided into four categories by Ae. biuncialis Y17, Ae. umbellulata Y139 and Ae. comosa Y258, including 172 markers amplifying the same bands of U and M genome, six and 102 markers amplifying U-specific and M-specific bands, respectively and eighteen markers amplifying specific bands in Y17. Among them, 45 markers had the specific amplifications in WA317 and were 5Mb specific markers. Taken together, line WA317 with tenacious and black glumes should serve as the foundation for understanding of the Triticeae domestication process and further exploitation of primitive alleles for wheat improvement. Ae. biuncialis SLAF-based markers can be used for studying syntenic relationships between U and M genomes as well as rapid tracking of U and M chromosomal segments in wheat background.

Download Full-text

Molecular Cytogenetic Analysis and Meiotic Pairing Behavior of Progenies Originating from a Hexaploid Triticale (×Triticosecale, Wittmack) and Bread Wheat (Triticum aestivum, L.) Cross

Cytogenetic and Genome Research ◽

10.1159/000506385 ◽

2020 ◽

Vol 160 (1) ◽

pp. 47-56

Author(s):

Aybeniz J. Aliyeva ◽

András Farkas ◽

Naib Kh. Aminov ◽

Klaudia Kruppa ◽

Márta Molnár-Láng ◽

...

Keyword(s):

In Situ Hybridization ◽

Chromosome Pairing ◽

Addition Line ◽

Meiotic Pairing ◽

Hexaploid Triticale ◽

Substitution Lines ◽

Molecular Cytogenetic ◽

Triticale Line ◽

Pairing Behavior

The chromosomal constitution of 9 dwarf (D) and 8 semidwarf (SD) lines derived by crossing hexaploid Triticale line NA-75 (AABBRR, 2n = 6x = 42) with Triticumaestivum (AABBDD, 2n = 6x = 42) cv. Chinese Spring was investigated using molecular cytogenetic techniques: fluorescence in situ hybridization and genomic in situ hybridization. A wheat-rye translocation (T4DS.7RL), 8 substitution lines, and a ditelosomic addition line (7RSdt) were identified. In the substitution lines, 1, 2, or 4 pairs of wheat chromosomes, belonging to the A, B, or D genome, were replaced by rye chromosomes. Substitutions between chromosomes belonging to different wheat genomes [5B(5A), 1D(1B)] also occurred. The lines were genetically stable, each carrying 42 chromosomes, except the wheat-rye ditelosomic addition line, which carried 21 pairs of wheat chromosomes and 1 pair of rye telocentric chromosomes (7RS). The chromosome pairing behavior of the lines was studied during metaphase I of meiosis. The chromosome pairing level and the number of ring bivalents were different for each line. Besides rod bivalents, univalent and multivalent associations (tri- and quadrivalents) were also detected. The main goal of the experiment was to develop genetically stable wheat/Triticale recombinant lines carrying chromosomes/chromatin fragments originating from the R genome of Triticale line NA-75. Introgression of rye genes into hexaploid wheat can broaden its genetic diversity, and the newly developed lines can be used in wheat breeding programs.

Download Full-text

Salt tolerance of two wheat – Agropyron junceum disomic addition lines

Genome ◽

10.1139/g88-094 ◽

1988 ◽

Vol 30 (4) ◽

pp. 559-564 ◽

Cited By ~ 55

Author(s):

B. P. Forster ◽

T. E. Miller ◽

C. N. Law

Keyword(s):

Salt Tolerance ◽

Chinese Spring ◽

Major Gene ◽

Addition Line ◽

Similar Response ◽

Addition Lines ◽

Disomic Addition Line ◽

Wheat Group ◽

Group 2 ◽

Disomic Addition Lines

Two wheat – Agropyron junceum disomic addition lines homoeologous to groups 2 and 5 were tested for tolerance to salt. The experiments included germination and growth to maturity at various concentrations of sodium chloride (NaCl). The results were compared with those of wheat lines tetrasomic for chromosomes 2A, 2B, 2D, 5A, 5B, and 5D and also with the wheat parent 'Chinese Spring', and the salt-tolerant 'Chinese Spring' – A. junceum amphiploid. The addition of homoeologous group 2 chromosomes reduced the tolerance to salt relative to 'Chinese Spring' in every case. The order of tolerance was ranked as 'Chinese Spring' > 2J disomic addition line > tetra 2A = tetra 2D > tetra 2B. The addition of wheat group 5 chromosomes was either equal to 'Chinese Spring' or worse with respect to tolerance to salt. However, the disomic addition line for 5J showed considerable tolerance to salt and at 200 mol m−3 NaCl produced a similar response to that of the amphiploid in producing fertile tillers. Both produced viable grain, but the grain produced by the 5J addition line at 200 mol−3 NaCl was small and shrivelled, unlike the plump grain produced by the amphiploid. The order of tolerance was ranked as amphiploid > 5J addition line > 'Chinese Spring' = tetra 5A > tetra 5B = tetra 5D. It is concluded that there are genes on the group 2 chromosomes that confer susceptibility to salt and that chromosome 5J of A. junceum carries a major gene(s) for tolerance to salt. The potential for transferring this character into wheat is discussed. A hypothesis is also proposed to explain the function of the salt-tolerance gene(s) at critical stages in the life cycle of wheat.Key words: salt tolerance, wheat, Agropyron junceum, disomic addition lines.

Download Full-text

Molecular cytogenetics identification of a wheat – Leymus mollis double disomic addition line with stripe rust resistance

Genome ◽

10.1139/gen-2016-0151 ◽

2017 ◽

Vol 60 (5) ◽

pp. 375-383 ◽

Cited By ~ 5

Author(s):

Aicen Zhang ◽

Wanyue Li ◽

Changyou Wang ◽

Xiaofei Yang ◽

Chunhuan Chen ◽

...

Keyword(s):

In Situ Hybridization ◽

Common Wheat ◽

Stripe Rust ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Fish Analysis ◽

Addition Line ◽

Disomic Addition Line ◽

Leymus Mollis

Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement. In the present study, we determined the karyotypic constitution of a wheat – L. mollis double disomic addition line, M11003-4-4-1-1, selected from the F5 progeny of a stable wheat – L. mollis derivative M39 (2n = 56) × Triticum aestivum cultivar 7182, by morphological and cytogenetic identification, GISH (genomic in situ hybridization), FISH (fluorescent in situ hybridization), molecular markers analysis, and stripe rust resistance evaluation. Cytological studies demonstrated that M11003-4-4-1-1 had a chromosome karyotype of 2n = 46 with 23 bivalents, while GISH and FISH analysis indicated that this line contained 42 common wheat chromosomes and two pairs of L. mollis chromosomes. DNA markers showed that the alien chromosomes from L. mollis belonged to homoeologous groups 5 and 6. Evaluation of the agronomic traits revealed that M11003-4-4-1-1 was resistant to stripe rust at the adult stage, while the plant height was reduced and the 1000-grain weight was increased significantly. Therefore, the new line M11003-4-4-1-1 could be exploited as an important bridge material in chromosome engineering and wheat breeding.

Download Full-text

Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance

PLoS ONE ◽

10.1371/journal.pone.0134534 ◽

2015 ◽

Vol 10 (8) ◽

pp. e0134534 ◽

Cited By ~ 17

Author(s):

Diaoguo An ◽

Qi Zheng ◽

Qiaoling Luo ◽

Pengtao Ma ◽

Hongxia Zhang ◽

...

Keyword(s):

Powdery Mildew ◽

Powdery Mildew Resistance ◽

Addition Line ◽

Mildew Resistance ◽

Disomic Addition Line ◽

Molecular Cytogenetic

Download Full-text

Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance

Molecular Breeding ◽

10.1007/s11032-016-0463-1 ◽

2016 ◽

Vol 36 (4) ◽

Cited By ~ 10

Author(s):

Yajuan Wang ◽

Wei Quan ◽

Nana Peng ◽

Changyou Wang ◽

Xiaofei Yang ◽

...

Keyword(s):

Powdery Mildew ◽

Powdery Mildew Resistance ◽

Addition Line ◽

Aegilops Geniculata ◽

Mildew Resistance ◽

Disomic Addition Line ◽

Molecular Cytogenetic ◽

Aegilops Geniculata Roth

Download Full-text

Molecular cytogenetic and agronomic characterization of advanced generations of wheat × triticale hybrids resistant to Diuraphis noxia (Mordvilko): application of GISH and microsatellite markers

Genome ◽

10.1139/g09-010 ◽

2009 ◽

Vol 52 (4) ◽

pp. 353-360 ◽

Cited By ~ 7

Author(s):

K. K. Nkongolo ◽

S. D. Haley ◽

N. S. Kim ◽

P. Michael ◽

G. Fedak ◽

...

Keyword(s):

Triticum Aestivum L ◽

Diuraphis Noxia ◽

Wheat Varieties ◽

Molecular Cytogenetic ◽

Triticale Line ◽

Chromosome 1R ◽

Disomic Addition Lines ◽

Donor Species

The PI 386148 triticale from Russia is among the highest resistant line to the Russian wheat aphid (RWA) ( Diuraphis noxia (Mordvilko)). This triticale line was used as the male parent in crosses with Lamar wheat ( Triticum aestivum L.). The F1 plants were backcrossed to Lamar wheat. The progenies were tested for RWA biotype 1 reaction for at least eight backcross and selfing generations. Five lines from these selections were identified for their resistance to the RWA and their seeds were increased for agronomic and other characterizations. Molecular and cytological analyses of these lines were performed using genomic in situ hybridization and rye chromosome-specific microsatellites markers. Three lines were cytologically stable and carried a pair of rye ( Secale strictum (C. Presl) C. Presl) chromosomes as disomic addition lines of 1R. One line was unstable and showed a moderate level of mixoploidy with monosomic additions of 1R. Duplication of rye chromosome 1R was also identified. No wheat–rye chromosome interchange was detected, suggesting little homology between S. strictum and T. aestivum chromosomes. Specific microsatellite primers were used to identify the rye chromosomes present in each line. One rye chromosome, 1R, from the donor species contains genes for RWA resistance. Grain yield and test weight of three of the lines were similar to some adapted released wheat varieties under stress conditions.

Download Full-text

Characterization of alien chromosomes in backcross derivatives of Triticum aestivum × Elymus rectisetus hybrids by using molecular markers and sequential multicolor FISH/GISH

Genome ◽

10.1139/g2012-018 ◽

2012 ◽

Vol 55 (5) ◽

pp. 337-347 ◽

Cited By ~ 7

Author(s):

Quan-Wen Dou ◽

Yunting Lei ◽

Xiaomei Li ◽

Ivan W. Mott ◽

Richard R.-C. Wang

Keyword(s):

Triticum Aestivum ◽

In Situ Hybridization ◽

Dna Sequences ◽

Tan Spot ◽

Stagonospora Nodorum ◽

Gene Pools ◽

Addition Line ◽

Disomic Addition Line ◽

Sts Markers

Wild Triticeae grasses serve as important gene pools for forage and cereal crops. Based on DNA sequences of genome-specific RAPD markers, sequence-tagged site (STS) markers specific for W and Y genomes have been obtained. Coupling with the use of genomic in situ hybridization, these STS markers enabled the identification of the W- and Y-genome chromosomes in backcross derivatives from hybrids of bread wheat Triticum aestivum L. (2n = 42; AABBDD) and Elymus rectisetus (Nees in Lehm.) Á. Löve & Connor (2n = 42; StStWWYY). The detection of six different alien chromosomes in five of these derivatives was ascertained by quantitative PCR of STS markers, simple sequence repeat markers, rDNA genes, and (or) multicolor florescence in situ hybridization. Disomic addition line 4687 (2n = 44) has the full complement of 42 wheat chromosomes and a pair of 1Y chromosomes that carry genes for resistance to tan spot (caused by Pyrenophora tritici-repentis (Died.) Drechs.) and Stagonospora nodorum blotch (caused by Stagonospora nodorum (Berk.) Castellani and Germano). The disomic addition line 4162 has a pair of 1St chromosomes and 21 pairs of wheat chromosomes. Lines 4319 and 5899 are two triple substitution lines (2n = 42) having the same chromosome composition, with 2A, 4B, and 6D of wheat substituted by one pair of W- and two pairs of St-genome chromosomes. Line 4434 is a substitution–addition line (2n = 44) that has the same W- and St-genome chromosomes substituting 2A, 4B, and 6D of wheat as in lines 4319 and 5899 but differs by having an additional pair of Y-genome chromosome, which is not the 1Y as in line 4687. The production and identification of these alien cytogenetic stocks may help locate and isolate genes for useful agronomic traits.

Download Full-text

Molecular cytogenetic characterization ofAegilops biuncialisand its use for the identification of 5 derived wheat – Aegilops biuncialisdisomic addition lines

Genome ◽

10.1139/g05-062 ◽

2005 ◽

Vol 48 (6) ◽

pp. 1070-1082 ◽

Cited By ~ 48

Author(s):

Annamária Schneider ◽

Gabriella Linc ◽

István Molnár ◽

Márta Molnár-Láng

Keyword(s):

Triticum Aestivum ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Genomic In Situ Hybridization ◽

Addition Lines ◽

Aegilops Umbellulata ◽

Aegilops Comosa ◽

Disomic Addition Lines ◽

M Genome

The aim of the experiments was to produce and identify different Triticum aestivum – Aegilops biuncialis disomic addition lines. To facilitate the exact identification of the Ae. biuncialis chromosomes in these Triticum aestivum – Ae. biuncialis disomic additions, it was necessary to analyze the fluorescence in situ hybridization (FISH) pattern of Ae. biuncialis (2n = 4x = 28, UbUbMbMb), comparing it with the diploid progenitors (Aegilops umbellulata, 2n = 2x = 14, UU and Aegilops comosa, 2n = 2x = 14, MM). To identify the Ae. biuncialis chromosomes, FISH was carried out using 2 DNA clones (pSc119.2 and pAs1) on Ae. biuncialis and its 2 diploid progenitor species. Differences in the hybridization patterns of all chromosomes were observed among the 4 Ae. umbellulata accessions, the 4 Ae. comosa accessions, and the 3 Ae. biuncialis accessions analyzed. The hybridization pattern of the M genome was more variable than that of the U genome. Five different wheat – Ae. biuncialis addition lines were produced from the wheat – Ae. biuncialis amphiploids produced earlier in Martonvásár. The 2M, 3M, 7M, 3U, and 5U chromosome pairs were identified with FISH using 3 repetitive DNA clones (pSc119.2, pAs1, and pTa71) in the disomic chromosome additions produced. Genomic in situ hybridization (GISH) was used to differentiate the Ae. biuncialis chromosomes from wheat, but no chromosome rearrangements between wheat and Ae. biuncialis were detected in the addition lines.Key words: Triticum aestivum, Aegilops biuncialis, fluorescence in situ hybridization, genomic in situ hybridization, wheat – Aegilops biuncialis addition lines.

Download Full-text