Parallel determination of glucocorticoid receptors in human mononuclear and polymorphonuclear leukocytes after Percoll separation

1985 ◽  
Vol 8 (1) ◽  
pp. 45-47 ◽  
Author(s):  
D. Armanini ◽  
T. Strasser ◽  
P. C. Weber
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Glucocorticoid Receptors

scholarly journals Clinical implications of glucocorticoid receptor studies in childhood acute lymphoblastic leukemia

Blood ◽  
1980 ◽  
Vol 56 (6) ◽  
pp. 1036-1040 ◽  
Author(s):  
R Mastrangelo ◽  
R Malandrino ◽  
R Riccardi ◽  
P Longo ◽  
FO Ranelletti ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Glucocorticoid Receptor ◽  
Glucocorticoid Receptors ◽  
Lymphoblastic Leukemia ◽  
Poor Response ◽  
The Other ◽  
Clinical Implications ◽  
Short Term

Abstract We have performed in parallel, in 19 children with acute lymphoblastic leukemia, a quantitative determination of glucocorticoid levels, in vitro steroid induced inhibition of nucleic acid precursors, and a short-term clinical trial of corticosteroids alone, before the treatment was given, which included corticosteroids and other drugs. From our results it appears that high glucocorticoid receptor levels in acute lymphoblastic leukemia of children do not guarantee a clinical response to corticosteroids. On the other hand, glucocorticoid receptors may turn out to be of value in predicting a poor response to corticosteroids only if their levels are considerably low.

scholarly journals Clinical implications of glucocorticoid receptor studies in childhood acute lymphoblastic leukemia

Blood ◽  
1980 ◽  
Vol 56 (6) ◽  
pp. 1036-1040 ◽  
Author(s):  
R Mastrangelo ◽  
R Malandrino ◽  
R Riccardi ◽  
P Longo ◽  
FO Ranelletti ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Glucocorticoid Receptor ◽  
Glucocorticoid Receptors ◽  
Lymphoblastic Leukemia ◽  
Poor Response ◽  
The Other ◽  
Clinical Implications ◽  
Short Term

We have performed in parallel, in 19 children with acute lymphoblastic leukemia, a quantitative determination of glucocorticoid levels, in vitro steroid induced inhibition of nucleic acid precursors, and a short-term clinical trial of corticosteroids alone, before the treatment was given, which included corticosteroids and other drugs. From our results it appears that high glucocorticoid receptor levels in acute lymphoblastic leukemia of children do not guarantee a clinical response to corticosteroids. On the other hand, glucocorticoid receptors may turn out to be of value in predicting a poor response to corticosteroids only if their levels are considerably low.

scholarly journals Semiautomated Method for Determination of Cystine Concentration in Polymorphonuclear Leukocytes

2000 ◽  
Vol 46 (4) ◽  
pp. 560-576 ◽  
Author(s):  
Anna Pastore ◽  
Anna Lo Russo ◽  
Marcella Greco ◽  
Gianfranco Rizzoni ◽  
Giorgio Federici
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Semiautomated Method

Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.

1976 ◽  
Vol 22 (10) ◽  
pp. 1618-1622 ◽  
Author(s):  
Y Houpert ◽  
P Tarallo ◽  
G Siest
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Polymorphonuclear Leukocytes ◽  
Extraction Procedure ◽  
Intracellular Pool ◽  
Human Polymorphonuclear Leukocytes

Abstract We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.

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