Predicting DON contamination in Fusarium-infected wheat grains via determination of the ergosterol content

2000 ◽  
Vol 28 (3) ◽  
pp. 337-344 ◽  
Author(s):  
Cs. Lamper ◽  
J. Téren ◽  
T. Bartók ◽  
R. Komoróczy ◽  
Á. Mesterházy ◽  
...  
Keyword(s):  
Wheat Grains ◽  
Ergosterol Content

Determination of wheat moisture using terahertz spectroscopy combined with tabu search algorithm

2021 ◽  
Author(s):  
Yin Shen ◽  
Chunjiang Zhao ◽  
Bin Li ◽  
Guanglin Li ◽  
Yanxin Yin ◽  
...  
Keyword(s):  
Tabu Search ◽  
Moisture Content ◽  
Terahertz Spectroscopy ◽  
Search Algorithm ◽  
Grain Storage ◽  
Tabu Search Algorithm ◽  
Wheat Grains

The detection of wheat moisture content plays a key role before grain storage and classification. The harvested wheat grains were taken as samples in the current research. A total of...

scholarly journals Quantifying Mold Biomass on Gypsum Board: Comparison of Ergosterol and Beta-N-Acetylhexosaminidase as Mold Biomass Parameters

2003 ◽  
Vol 69 (7) ◽  
pp. 3996-3998 ◽  
Author(s):  
M. Reeslev ◽  
M. Miller ◽  
K. F. Nielsen
Keyword(s):  
Stationary Phase ◽  
Linear Correlation ◽  
Stachybotrys Chartarum ◽  
Gypsum Board ◽  
Biomass Density ◽  
Activity Data ◽  
Good Linear ◽  
Ergosterol Content ◽  
Good Linear Correlation

ABSTRACT Two mold species, Stachybotrys chartarum and Aspergillus versicolor, were inoculated onto agar overlaid with cellophane, allowing determination of a direct measurement of biomass density by weighing. Biomass density, ergosterol content, and beta-N-acetylhexosaminidase (3.2.1.52) activity were monitored from inoculation to stationary phase. Regression analysis showed a good linear correlation to biomass density for both ergosterol content and beta-N-acetylhexosaminidase activity. The same two mold species were inoculated onto wallpapered gypsum board, from which a direct biomass measurement was not possible. Growth was measured as an increase in ergosterol content and beta-N-acetylhexosaminidase activity. A good linear correlation was seen between ergosterol content and beta-N-acetylhexosaminidase activity. From the experiments performed on agar medium, conversion factors (CFs) for estimating biomass density from ergosterol content and beta-N-acetylhexosaminidase activity were determined. The CFs were used to estimate the biomass density of the molds grown on gypsum board. The biomass densities estimated from ergosterol content and beta-N-acetylhexosaminidase activity data gave similar results, showing significantly slower growth and lower stationary-phase biomass density on gypsum board than on agar.

Development of fluorescence polarization immunoassays for parallel detection of pesticides carbaryl and triazophos in wheat grains

2017 ◽  
Vol 9 (48) ◽  
pp. 6814-6822 ◽  
Author(s):  
Anna Yu Boroduleva ◽  
Jing Wu ◽  
Qingqing Yang ◽  
Hui Li ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Wheat Grains ◽  
Parallel Detection

Fluorescence polarization immunoassays (FPIA) were developed for the determination of pesticides triazophos and carbaryl in wheat grains using the portable FPIA device Sentry 200 (Ellie).

scholarly journals Determination of Ochratoxin A (OTA), Ochratoxin B (OTB), T-2, and HT-2 Toxins in Wheat Grains, Wheat Flour, and Bread in Lebanon by LC-MS/MS

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 471 ◽  
Author(s):  
Jomana Elaridi ◽  
Osama Yamani ◽  
Amira Al Matari ◽  
Saada Dakroub ◽  
Zouhair Attieh
Keyword(s):  
Ochratoxin A ◽  
Wheat Flour ◽  
Health Concern ◽  
The European Union ◽  
Public Health Concern ◽  
Wheat Grains ◽  
Mycotoxin Contamination ◽  
Ochratoxin B ◽  
Interday Precision

Cereals are prone to fungal infection during growth, harvesting, transportation, and/or storage. As a result, cereals such as wheat grains and wheat-derived products may be contaminated with mycotoxins leading to acute and chronic health exposure. The current study investigated the presence of the mycotoxins: ochratoxin A (OTA), ochratoxin B (OTB), T-2, and HT-2 toxins in samples of wheat grains (n = 50), wheat flour (n = 50), and bread (n = 37) from the main mills in Lebanon using LC-MS/MS. Accuracy ranged from 98–100%, recoveries from 93–105%, and intraday and interday precision were 5–7% and 9–12%, respectively. The tested wheat grains, wheat flour, and bread samples did not contain detectable levels of T-2 and HT-2 toxins and OTB. Four wheat flour samples (8% of flour samples) showed positive OTA levels ranging from 0.6–3.4 μg·kg−1 with an arithmetic mean of 1.9 ± 0.2 μg·kg−1. Only one sample contained an OTA concentration greater than the limit set by the European Union (3 μg·kg−1) for wheat-derived products. This study suggests that mycotoxin contamination of wheat grains, wheat flour, and bread in Lebanon is currently not a serious public health concern. However, surveillance strategies and monitoring programs must be routinely implemented to ensure minimal mycotoxin contamination of wheat-based products.

Development of a fast multiresidue method for the determination of pesticides in dry samples (wheat grains, flour and bran) using QuEChERS based method and GC–MS

2011 ◽  
Vol 125 (4) ◽  
pp. 1436-1442 ◽  
Author(s):  
Diana I. Kolberg ◽  
Osmar D. Prestes ◽  
Martha B. Adaime ◽  
Renato Zanella
Keyword(s):  
Wheat Grains ◽  
Multiresidue Method

scholarly journals Dynamic Interaction between Fluconazole and Amphotericin B against Cryptococcus gattii

2012 ◽  
Vol 56 (5) ◽  
pp. 2553-2558 ◽  
Author(s):  
Julliana Ribeiro Alves Santos ◽  
Ludmila Ferreira Gouveia ◽  
Erika Linzi Silva Taylor ◽  
Maria Aparecida Resende-Stoianoff ◽  
Gerson Antônio Pianetti ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Cryptococcus Gattii ◽  
Content Type ◽  
Antagonistic Interaction ◽  
Ergosterol Content ◽  
Kill Curve ◽  
Time Kill ◽  
Postantifungal Effect

ABSTRACTCryptococcus gattiiis the main pathogen of cryptococcosis in healthy patients and is treated mainly with fluconazole and amphotericin B. The combination of these drugs has been questioned because the mechanisms of action could lead to a theoretical antagonistic interaction. We evaluated distinct parameters involved in thein vitrocombination of fluconazole and amphotericin B againstCryptococcus gattii. Fourteen strains ofC. gattiiwere used for the determination of MIC, fractional inhibitory concentration, time-kill curve, and postantifungal effect (PAFE). Ergosterol quantification was performed to evaluate the influence of ergosterol content on the interaction between these antifungals. Interaction between the drugs varied from synergistic to antagonistic depending on the strain and concentration tested. Increasing fluconazole levels were correlated with an antagonistic interaction. A total of 48 h was necessary for reducing the fungal viability in the presence of fluconazole, while 12 h were required for amphotericin B. When these antifungals were tested in combination, fluconazole impaired the amphotericin B activity. The ergosterol content decreased with the increase of fluconazole levels and it was correlated with the lower activity of amphotericin B. The PAFE found varied from 1 to 4 h for fluconazole and from 1 to 3 h for amphotericin B. The interaction of fluconazole and amphotericin B was concentration-dependent and special attention should be directed when these drugs are used in combination againstC. gattii.

A rapid method for the determination of malathion in wheat grains

The Analyst ◽  
1968 ◽  
Vol 93 (1108) ◽  
pp. 443 ◽  
Author(s):  
E. Weisenberg ◽  
S. Gertner ◽  
J. Schoenberg
Keyword(s):  
Rapid Method ◽  
Wheat Grains
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