Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification

2003 ◽  
Vol 60 (8) ◽  
pp. 1529-1546 ◽  
Author(s):  
V. Sarramegna ◽  
F. Talmont ◽  
P. Demange ◽  
A. Milon
Keyword(s):  
Heterologous Expression ◽  
G Protein ◽  
Large Scale ◽  
G Protein Coupled Receptors ◽  
Scale Production ◽  
Expression Systems ◽  
Large Scale Production ◽  
G Protein Coupled

scholarly journals Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4

2009 ◽  
Vol 106 (29) ◽  
pp. 11925-11930 ◽  
Author(s):  
B. L. Cook ◽  
D. Steuerwald ◽  
L. Kaiser ◽  
J. Graveland-Bikker ◽  
M. Vanberghem ◽  
...  
Keyword(s):  
G Protein ◽  
Olfactory Receptor ◽  
Large Scale ◽  
Scale Production ◽  
G Protein Coupled Receptor ◽  
Large Scale Production ◽  
G Protein Coupled

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies

2009 ◽  
Vol 386 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Kerstin Michalke ◽  
Marie-Eve Gravière ◽  
Céline Huyghe ◽  
Renaud Vincentelli ◽  
Renaud Wagner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
G Protein ◽  
High Throughput ◽  
Inclusion Bodies ◽  
Large Scale ◽  
Receptor Expression ◽  
Scale Production ◽  
Expression In Escherichia Coli ◽  
Large Scale Production ◽  
G Protein Coupled

Heterologous Expression of G Protein?Coupled Receptors in U-2 OS Osteosarcoma Cells

2004 ◽  
Vol 10 (3-4) ◽  
pp. 117-124 ◽  
Author(s):  
Robert Ames ◽  
Parvathi Nuthulaganti ◽  
Jim Fornwald ◽  
Usman Shabon ◽  
Harjeet van-der-Keyl ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
G Protein ◽  
G Protein Coupled Receptors ◽  
Osteosarcoma Cells ◽  
G Protein Coupled

scholarly journals Plant expression systems for production of hemagglutinin as a vaccine against influenza virus.

2014 ◽  
Vol 61 (3) ◽  
Author(s):  
Patrycja Redkiewicz ◽  
Agnieszka Sirko ◽  
Katarzyna Anna Kamel ◽  
Anna Góra-Sochacka
Keyword(s):  
Influenza Virus ◽  
Large Scale ◽  
Immunological Response ◽  
Biologically Active ◽  
Scale Production ◽  
Expression Systems ◽  
Lethal Challenge ◽  
Subunit Vaccines ◽  
Large Scale Production ◽  
Major Surface

Many examples of a successful application of plant-based expression systems for production of biologically active recombinant proteins exist in the literature. These systems can function as inexpensive platforms for the large scale production of recombinant pharmaceuticals or subunit vaccines. Hemagglutinin (HA) is a major surface antigen of the influenza virus, thus it is in the centre of interests of various subunit vaccine engineering programs. Large scale production of recombinant HA in traditional expression systems, such as mammalian or insect cells, besides other limitations, is expensive and time-consuming. These difficulties stimulate an ever-increasing interest in plant-based production of this recombinant protein. Over the last few years many successful cases of HA production in plants, using both transient and stable expression systems have been reported. Various forms of recombinant HA, including monomers, trimers, virus like particles (VLPs) or chimeric proteins containing its fusion with other polypeptides were obtained and shown to maintain a proper antigenicity. Immunizations of animals (mice, ferrets, rabbits or chickens) with some of these plant-derived hemagglutinin variants were performed, and their effectiveness in induction of immunological response and protection against lethal challenge with influenza virus demonstrated. Plant-produced recombinant subunit vaccines and plant-made VLPs were successfully tested in clinical trials (Phase I and II) that confirmed their tolerance and immunogenicity.

scholarly journals Heterologous Expression and Purification Systems for Structural Proteomics of Mammalian Membrane Proteins

2002 ◽  
Vol 3 (6) ◽  
pp. 511-517 ◽  
Author(s):  
Isabelle Mus-Veteau
Keyword(s):  
Membrane Proteins ◽  
Heterologous Expression ◽  
Large Scale ◽  
Structural Information ◽  
Structural Data ◽  
Structural Proteomics ◽  
Scale Production ◽  
Expression And Purification ◽  
Large Scale Production ◽  
Heterologous Expression Systems

Membrane proteins (MPs) are responsible for the interface between the exterior and the interior of the cell. These proteins are implicated in numerous diseases, such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, heart failure, hypertension and Alzheimer's disease. However, studies on these disorders are hampered by a lack of structural information about the proteins involved. Structural analysis requires large quantities of pure and active proteins. The majority of medically and pharmaceutically relevant MPs are present in tissues at very low concentration, which makes heterologous expression in large-scale production-adapted cells a prerequisite for structural studies. Obtaining mammalian MP structural data depends on the development of methods that allow the production of large quantities of MPs. This review focuses on the different heterologous expression systems, and the purification strategies, used to produce large amounts of pure mammalian MPs for structural proteomics.

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