Biochemical evaluation of antihyperglycemic and antioxidative effects of Morinda citrifolia fruit extract studied in streptozotocin-induced diabetic rats

Crocidolomia binotalis Zell is an important pest in Brassicaceae vegetable crops. Vegetable insecticide is one potential alternative to controlling the pest. This study aims to determine the application time of noni fruit extract and to determine the effective concentration of noni fruit extract as a vegetable insecticide in controlling C. binotalis larva on cabbage plants in the field. this study used a Completely Randomized Design (RAL) consisting of two treatment factors and four replications. The first factor is the concentration of extract (5 ppm, 10 ppm, 15 ppm and 20 ppm) and the second factor is the time of application of vegetable pesticide before the pest is invested (W1) and after the pest is invested (W2). So there are 32 units of experiments and 4 units of experiments added as control. The results showed, noni fruit extract had no significant effect on mortality of C. binotalis larvae. The highest mortality rate is at 100% K2 (10ppm) concentration at the time of application before the pest is invested (W1). The interaction had significant effect on the wet weight of the plant canopy but had no significant effect on the mortality of the pest, the intensity of the attack, the percentage of pupa formed, and the present percentage of imago.

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Improvements in HOMA indices and pancreatic endocrinal tissues in type 2-diabetic rats by DPP-4 inhibition and antioxidant potential of an ethanol fruit extract of Withania coagulans

10.21203/rs.3.rs-69981/v2 ◽

2021 ◽

Author(s):

Heera Ram ◽

Pramod Kumar ◽

Ashok Purohit ◽

Priya Kashyap ◽

Suresh Kumar ◽

...

Keyword(s):

Diabetic Rats ◽

Model Assessment ◽

Fruit Extract ◽

Protein Ligands ◽

Homeostatic Model Assessment ◽

Docking Protein ◽

Type 2 Diabetic

Abstract Context: Withania coagulans (Stocks) Dunal fruits are used in the therapeutics of several ailments due to possessing of potent phytoconstituents which is also used traditionally for curing the diabetes. Objective: The present study was assessing the amelioration potential of the phytochemicals of an ethanol fruit extract of Withania coagulans (Stocks) Dunal in the HOMA (Homeostatic model assessment) indices and pancreatic endocrinal tissues by inhibition of DPP-4 and antioxidants activities.Material and methods: The identification of phytoconstituents of the test extract was performed by LCMS. Further, assessments of in-vitro, in-vivo and in-silico were achieved by following standard methods. In-vivo studies were conducted on type-2 diabetic ratsResults: The chosen extract inhibited DPP-4 activity by 63.2% in an in vitro assay as well as significantly inhibit serum DPP-4 levels. Accordingly, the administration of the ethanol fruit extract resulted in a significant (𝑃≤ 0.001) alterations in the lipid profile, antioxidant levels, and HOMA indices. Moreover, pancreatic endocrinal tissues (islet of Langerhans) appeared to have the restoration of normal histoarchitecture as evidenced by increased cellular mass. Molecular docking (Protein - ligands) of identified phytoconstituents with DPP-4 (target enzyme) shown incredibly low binding energy (Kcal/mol) as required for ideal interactions. ADMET analysis of the pharmacokinetics of the identified phytoconstituents indicated an ideal profile as per Lipinski laws. Conclusion: It can be concluded that the phytoconstituents of an ethanol fruit extract of Withania coagulans have the potential to inhibit DPP-4 which result in improved glucose homeostasis and restoration of pancreatic endocrinal tissues in type-2 diabetic rats.

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Improvements in HOMA Indices and Pancreatic Endocrinal Tissues in Type -2 Diabetic Rats by DPP-4 Inhibition and Antioxidant Potential of an Ethanol Fruit Extract of Withania Coagulans

10.21203/rs.3.rs-69981/v1 ◽

2020 ◽

Author(s):

Heera Ram ◽

Pramod Kumar ◽

Ashok Purohit ◽

Priya Kashyap ◽

Suresh Kumar ◽

...

Keyword(s):

Diabetic Rats ◽

Model Assessment ◽

Fruit Extract ◽

Withania Coagulans ◽

Homeostatic Model Assessment ◽

Docking Protein ◽

Type 2 Diabetic

Abstract Context: Withania coagulans (Stocks) Dunal fruits are used in the therapeutics of several ailments due to possessing of potent phytoconstituents which is also used traditionally for curing the diabetes. Objective: The present study was assessing the amelioration potential of the phytochemicals of an ethanol fruit extract of Withania coagulans (Stocks) Dunal in the HOMA (Homeostatic model assessment) indices and pancreatic endocrinal tissues by inhibition of DPP-4 and antioxidants activities.Material and methods: The identification of phytoconstituents of phytochemicals of the test extract was performed by LCMS. Further, assessments of in-vitro, in-vivo and in-silico were achieved by following standard methods. In-vivo studies were conducted on type-2 diabetic ratsResults: The chosen extract inhibited DPP-4 activity by 63.2% in an in vitro assay. Accordingly, the administration of the ethanol fruit extract resulted in a significant (𝑃≤ 0.001) alterations in the lipid profile, antioxidant levels, and HOMA indices. Moreover, pancreatic endocrinal tissues (islet of Langerhans) appeared to have the restoration of normal histoarchitecture as evidenced by increased cellular mass. Molecular docking (Protein - ligands) of identified phytoconstituents with DPP-4 (target enzyme) shown incredibly low binding energy (Kcal/mol) as required for ideal interactions. ADMET analysis of the pharmacokinetics of the identified phytoconstituents indicated an ideal profile as per Lipinski laws. Conclusion: It can be concluded that the phytoconstituents of an ethanol fruit extract of Withania coagulans have the potential to inhibit DPP-4 which result in improved glucose homeostasis and restoration of pancreatic endocrinal tissues in type-2 diabetic rats.

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