Antioxidative effects of proteglycans of embryonic genesis in streptozotocin-induced diabetic rats

Objective: The antihyperglycemic and antioxidative effects of L. microphyllum were evaluated by using in vivo methods in normal and alloxan induced diabetic rats.Methods: Diabetes was induced in Sprague Dawley rats by injecting alloxan through intravenous (i. v) at a dose of 100 mg/kg of body weight. Aqueous extract of L. microphyllum at different doses (400, 200 and 100 mg/kg of body weight) was administered orally (orogastric intubation) for 14 d. Blood glucose and oxidative stress markers were measured. Hematoxylin and eosin staining method were used to examine the pancreatic tissues.Results: At the 14 d interval, fasting blood glucose showed a reduction in serum glucose levels in animals pretreated with L. microphyllum compared with alloxan alone treated group. Oxidative stress was noticed in rat’s pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-S-transferase, and catalase activities. Malondialdehyde showed a significant increase compared to the normal saline-treated control group. Serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 d significantly prevented these alterations and attenuated alloxan-induced oxidative stress (P<0.05).Conclusion: The results of the present study indicated that the antihyperglycemic potential of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it is concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress.

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Nicorandil prevents endothelial dysfunction due to antioxidative effects via normalisation of NADPH oxidase and nitric oxide synthase in streptozotocin diabetic rats

Cardiovascular Diabetology ◽

10.1186/1475-2840-10-105 ◽

2011 ◽

Vol 10 (1) ◽

pp. 105 ◽

Cited By ~ 46

Author(s):

Ken-ichi Serizawa ◽

Kenji Yogo ◽

Ken Aizawa ◽

Yoshihito Tashiro ◽

Nobuhiko Ishizuka

Keyword(s):

Nitric Oxide ◽

Endothelial Dysfunction ◽

Nitric Oxide Synthase ◽

Nadph Oxidase ◽

Diabetic Rats ◽

Antioxidative Effects ◽

Streptozotocin Diabetic Rats ◽

Oxide Synthase

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Biochemical evaluation of antihyperglycemic and antioxidative effects of Morinda citrifolia fruit extract studied in streptozotocin-induced diabetic rats

Medicinal Chemistry Research ◽

10.1007/s00044-008-9140-1 ◽

2008 ◽

Vol 18 (6) ◽

pp. 433-446 ◽

Cited By ~ 10

Author(s):

U. S. Mahadeva Rao ◽

S. Subramanian

Keyword(s):

Diabetic Rats ◽

Morinda Citrifolia ◽

Fruit Extract ◽

Antioxidative Effects ◽

Biochemical Evaluation

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Studies on the Antidiabetic Activities ofCordyceps militarisExtract in Diet-Streptozotocin-Induced Diabetic Sprague-Dawley Rats

BioMed Research International ◽

10.1155/2014/160980 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 32

Author(s):

Yuan Dong ◽

Tianjiao Jing ◽

Qingfan Meng ◽

Chungang Liu ◽

Shuang Hu ◽

...

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Water Extract ◽

Diabetic Rats ◽

Cordyceps Militaris ◽

Sprague Dawley ◽

Alcohol Extract ◽

Glucose Levels ◽

Liver And Kidney ◽

Antioxidative Effects

Due to substantial morbidity and high complications, diabetes mellitus is considered as the third “killer” in the world. A search for alternative antidiabetic drugs from herbs or fungi is highly demanded. Our present study aims to investigate the antidiabetic activities ofCordyceps militarison diet-streptozotocin-induced type 2 diabetes mellitus in rats. Diabetic rats were orally administered with water extract or alcohol extract at 0.05 g/kg and 2 g/kg for 3 weeks, and then, the factors levels related to blood glucose, lipid, free radicals, and even nephropathy were determined. Pathological alterations on liver and kidney were examined. Data showed that, similar to metformin,Cordyceps militarisextracts displayed a significant reduction in blood glucose levels by promoting glucose metabolism and strongly suppressed total cholesterol and triglycerides concentration in serum.Cordyceps militarisextracts exhibit antioxidative effects indicated by normalized superoxide dismutase and glutathione peroxidase levels. The inhibitory effects on blood urea nitrogen, creatinine, uric acid, and protein revealed the protection ofCordyceps militarisextracts against diabetic nephropathy, which was confirmed by pathological morphology reversion. Collectively,Cordyceps militarisextract, a safe pharmaceutical agent, presents excellent antidiabetic and antinephropathic activities and thus has great potential as a new source for diabetes treatment.

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