The roles of endogenous reactive oxygen species and nitric oxide in triptolide-induced apoptotic cell death in macrophages

2006 ◽  
Vol 85 (1) ◽  
pp. 85-98 ◽  
Author(s):  
Xiaofeng Bao ◽  
Jun Cui ◽  
Yuanyuan Wu ◽  
Xiaodong Han ◽  
Cheng Gao ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Reactive Oxygen ◽  
Apoptotic Cell Death ◽  
Oxygen Species ◽  
Endogenous Reactive Oxygen Species

scholarly journals Nitric Oxide and Reactive Oxygen Species Do Not Elicit Hypersensitive Cell Death but Induce Apoptosis in the Adjacent Cells During the Defense Response of Oat

2004 ◽  
Vol 17 (3) ◽  
pp. 245-253 ◽  
Author(s):  
Yasuomi Tada ◽  
Tomoyo Mori ◽  
Takeshi Shinogi ◽  
Nan Yao ◽  
Satsuki Takahashi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Cell Death ◽  
Defense Response ◽  
Apoptotic Cell ◽  
Reactive Oxygen ◽  
Apoptotic Cell Death ◽  
Oxygen Species ◽  
Hypersensitive Cell Death ◽  
Dna Laddering

Nitric oxide (NO) acts as a signaling molecule in many cellular responses in plants and animals. Oat plants (Avena sativa L.) evoke the hypersensitive response (HR), which shares morphological and biochemical features with mammalian apoptosis, such as DNA laddering and heterochromatin condensation, in response to the avirulent crown rust fungus (Puccinia coronata f. sp. avenae). We examined the role of NO and reactive oxygen species (ROS) in the initiation of hypersensitive cell death, which is induced by direct contact with the pathogen, and apoptotic cell death in the adjacent cells. Cytofluorimetric analysis using the fluorescent NO probe DAF and the H2O2 probe DCF demonstrated that NO and H2O2 were generated simultaneously in primary leaves at an early stage of the defense response. The NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) markedly enhanced H2O2 accumulation detected by 3,3-diaminobenzidine staining and DCF, whereas treatment with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) strongly suppressed it. Superoxide dismutase (SOD) increased NO accumulation, suggesting that endogenous NO may modulate the level of H2O2 by interacting with O2 - in the HR lesion. Cytological observation showed that administration of cPTIO, SNAP, or SOD had no effect on elicitation of hypersensitive cell death, but clearly reduced heterochromatin condensation in the nearby cells and DNA laddering. These findings indicate that NO and ROS are not essential mediators for the initiation of hypersensitive cell death. However, NO and O2 - but not H2O2 are required for the onset of apoptotic cell death in the adjacent cells, where excess NO may exert its anti-apoptotic function by regulating cellular redox state.

scholarly journals cis-Urocanic Acid Enhances Prostaglandin E2 Release and Apoptotic Cell Death via Reactive Oxygen Species in Human Keratinocytes

2011 ◽  
Vol 131 (6) ◽  
pp. 1262-1271 ◽  
Author(s):  
Kazuyo Kaneko ◽  
Susan L. Walker ◽  
Joey Lai-Cheong ◽  
Mary S. Matsui ◽  
Mary Norval ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Prostaglandin E2 ◽  
Apoptotic Cell ◽  
Reactive Oxygen ◽  
Human Keratinocytes ◽  
Urocanic Acid ◽  
Apoptotic Cell Death ◽  
Oxygen Species

scholarly journals Green-Synthesized Silver Nanoparticles Induced Apoptotic Cell Death in MCF-7 Breast Cancer Cells by Generating Reactive Oxygen Species and Activating Caspase 3 and 9 Enzyme Activities

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Ikram Ullah ◽  
Ali Talha Khalil ◽  
Muhammad Ali ◽  
Javed Iqbal ◽  
Waqar Ali ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Silver Nanoparticles ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Reactive Oxygen ◽  
Apoptotic Cell Death ◽  
Oxygen Species ◽  
Cell Viability Assay ◽  
Mcf 7

Silver nanoparticles are among the most significant diagnostic and therapeutic agents in the field of nanomedicines. In the current study, the green chemistry approach was made to optimize a cost-effective synthesis protocol for silver nanoparticles from the aqueous extract of the important anticancer plant Fagonia indica. We investigated the anticancer potential and possible involvement of AgNPs in apoptosis. The biosynthesized AgNPs are stable (zeta potential, -16.3 mV) and spherical with a crystal size range from 10 to 60 nm. The MTT cell viability assay shows concentration-dependent inhibition of the growth of Michigan Cancer Foundation-7 (MCF-7) cells (IC50, 12.35 μg/mL). In addition, the fluorescent microscopic analysis shows activation of caspases 3 and 9 by AgNPs that cause morphological changes (AO/EB assay) in the cell membrane and cause nuclear condensation (DAPI assay) that eventually lead to apoptotic cell death (Annexin V/PI assay). It was also observed that AgNPs generate reactive oxygen species (ROS) that modulate oxidative stress in MCF-7 cells. This is the first study that reports the synthesis of a silver nanoparticle mediated by Fagonia indica extract and evaluation of the cellular and molecular mechanism of apoptosis.

scholarly journals Silibinin Induced Autophagic and Apoptotic Cell Death in HT1080 Cells Through a Reactive Oxygen Species Pathway

2010 ◽  
Vol 113 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Wenjun Duan ◽  
Xiaoying Jin ◽  
Qisheng Li ◽  
Shin-ichi Tashiro ◽  
Satoshi Onodera ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Reactive Oxygen ◽  
Apoptotic Cell Death ◽  
Oxygen Species ◽  
Ht1080 Cells

scholarly journals Death Receptors 4 and 5 Activate Nox1 NADPH Oxidase through Riboflavin Kinase to Induce Reactive Oxygen Species-mediated Apoptotic Cell Death

2011 ◽  
Vol 287 (5) ◽  
pp. 3313-3325 ◽  
Author(s):  
Kyung-Jin Park ◽  
Chang-Han Lee ◽  
Aeyung Kim ◽  
Ki Jun Jeong ◽  
Chul-Ho Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Nadph Oxidase ◽  
Apoptotic Cell ◽  
Reactive Oxygen ◽  
Death Receptors ◽  
Apoptotic Cell Death ◽  
Oxygen Species
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