Genetics of virulence in Leptosphaeria maculans (Desm.) Ces. et De Not., the cause of blackleg in rapeseed (Brassica napus L.)

1996 ◽  
Vol 93 (3) ◽  
pp. 301-306 ◽  
Author(s):  
E. C. K. Pang ◽  
G. M. Halloran
Keyword(s):  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Brassica Napus L

Development of an agronomically superior blackleg resistant canola cultivar in Brassica napus L. using doubled haploidy

1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari
Keyword(s):  
Brassica Napus ◽  
Doubled Haploid ◽  
Leptosphaeria Maculans ◽  
Resistance Breeding ◽  
Breeding Method ◽  
Brassica Napus L ◽  
Breeding Lines ◽  
University Of Alberta ◽  
Blackleg Resistance ◽  
The University

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus

Association mapping of quantitative resistance for Leptosphaeria maculans in oilseed rape (Brassica napus L.)

2010 ◽  
Vol 27 (3) ◽  
pp. 271-287 ◽  
Author(s):  
C. Jestin ◽  
M. Lodé ◽  
P. Vallée ◽  
C. Domin ◽  
C. Falentin ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Oilseed Rape ◽  
Quantitative Resistance ◽  
Leptosphaeria Maculans ◽  
Brassica Napus L

The teleomorph of the weakly aggressive segregate ofLeptosphaeriamaculans

2001 ◽  
Vol 79 (4) ◽  
pp. 412-419 ◽  
Author(s):  
R A Shoemaker ◽  
Hortense Brun
Keyword(s):  
New Species ◽  
Brassica Napus ◽  
Helianthus Annuus ◽  
Leptosphaeria Maculans ◽  
Interspecific Crosses ◽  
Phoma Lingam ◽  
Type Specimens ◽  
Brassica Napus L ◽  
Weakly Virulent ◽  
Leptosphaeria Biglobosa

Leptosphaeria biglobosa n.sp. is described for the weakly virulent (or non-aggressive) pathogen causing blackleg of oilseed rape and canola. The new species differs from the aggressive blackleg organism, Leptosphaeria maculans (Desm.) Ces. & De Not. in having a very prominent beak on the ascomata that is greatly enlarged at the apex. Ascomata of both species were produced on canola stems either by inoculation of compatible strains of the Phoma anamorph or by single ascospore isolates at a distance of 1 cm and letting them grow together. Both species exhibit bipolar heterothallism. Interspecific crosses were negative. In a study of type specimens of Leptosphaeria species with 5-septate ascospores infecting Cruciferae, none were found that matched the features of L. biglobosa. Leptosphaeria napi (Fuckel) Saccardo on Brassica napus L., and Leptosphaeria virginica (Cooke & Ellis) Saccardo on Lepidium virginica L., are synonyms of L. maculans. Leptosphaeria submaculans Holm on Sisymbrium, Berteroa, and Erysimum has distinctively enlarged ascospores. Leptosphaeria alliariae (Desm.) Ces. & De Not. on Alliaria officinalis Andrz., has much smaller ascospores. Leptosphaeria lindquistii Frezzi on Helianthus annuus L. of the Asteraceae has ascospores with only one, two, or rarely, three septa but was considered because it too has a Phoma anamorph.Key words: Phoma lingam, B group, ascomata.

Molecular mapping and validation of Rlm1 gene for resistance to Leptosphaeria maculans in canola (Brassica napus L.)

2012 ◽  
Vol 63 (10) ◽  
pp. 1007 ◽  
Author(s):  
Rosy Raman ◽  
Belinda Taylor ◽  
Kurt Lindbeck ◽  
Neil Coombes ◽  
Denise Barbulescu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Ssr Markers ◽  
Genomic Sequence ◽  
Molecular Mapping ◽  
Durable Resistance ◽  
Leptosphaeria Maculans ◽  
Doubled Haploid Population ◽  
Brassica Napus L ◽  
Breeding Populations ◽  
Independent Population

European winter canola (Brassica napus L.) cultivars harbour genes for durable resistance to the fungus Leptosphaeria maculans, which causes blackleg disease under Australian environmental conditions. Previous studies have shown that resistance in winter-type cultivars Maxol and Columbus is controlled by two genes, Rlm1 and Rlm3, which have been mapped using randomly amplified polymorphic DNA markers onto chromosome A7. We mapped a doubled-haploid population that consisted of 101 lines from a cross between Maxol*1 and Westar-10 using diversity arrays technology and simple sequence repeat (SSR)-based markers. Two SSR marker loci, Xol12-e03 and Xra2-a05b, flanked the Rlm1 locus at an interval of 6.7 cM, which corresponds to ~3.2 Mb of the Brassica rapa genomic sequence; this region contains several genes encoding putative kinase and leucine-rich repeat-type disease-resistance proteins. SSR markers were further tested for their linkage with the Rlm1 locus in an independent population derived from Columbus*3/Westar-10. Our results showed that SSR markers linked to Rlm1 can be useful for monitoring Rlm1 gene introgression in breeding populations derived from Maxol and Columbus.

Evaluation of pyraclostrobin as a component in seed and foliar fungicides for the management of blackleg (Leptosphaeria maculans) of canola (Brassica napus)

2020 ◽  
Vol 100 (5) ◽  
pp. 549-559
Author(s):  
Michelle Fraser ◽  
Stephen E. Strelkov ◽  
George D. Turnbull ◽  
Hafiz U. Ahmed ◽  
Wayne Barton ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Untreated Control ◽  
Seed Treatment ◽  
Fungal Pathogens ◽  
Leptosphaeria Maculans ◽  
Susceptible Cultivar ◽  
Brassica Napus L ◽  
Yield Increase ◽  
Novel Target ◽  
Stem Cankers

Infection by Leptosphaeria maculans (Desm.) Ces. & de Not., the causal agent of blackleg of canola (Brassica napus L.), can result in seedling death, foliar lesions, and the development of stem cankers on adult plants. Strobilurin fungicides such as pyraclostrobin are popular, as they are effective against a broad range of fungal pathogens, have novel target specificity, and translaminar activity within the plant. The seed treatment Prosper FX (carbathiin + trifloxystrobin + metalaxyl) and an experimental formulation BAS 720 F (pyraclostrobin + fluxapyroxad + metalaxyl) were evaluated for their efficacy in managing blackleg when applied alone or in combination with the foliar fungicides Priaxor (pyraclostrobin + fluxapyroxad) or Tilt 250 EC (propiconazole). Experiments were conducted with the blackleg-susceptible cultivar ‘Westar’ and the moderately resistant cultivar ‘Dekalb 73-15 RR’ under greenhouse and field conditions in 2012 and 2013. In one greenhouse trial, all fungicide treatments significantly reduced stem blackleg severity on ‘73-15’ and ‘Westar’ compared with the inoculated–untreated controls. During 3 of 4 site-years, the combination of the experimental seed treatment BAS 720 F and Priaxor significantly reduced stem disease severity relative to the inoculated–untreated control by 60%–77% for ‘73-15’ and by 50%–75% for ‘Westar’. This treatment also resulted in a significant yield increase relative to the inoculated–untreated control in ‘Westar’ in 2 site-years by 24% and 56%. The results of the study suggest a reduction in blackleg symptoms and improved yields when canola is treated with a combination of seed and foliar fungicides, especially when cropping a susceptible cultivar.

scholarly journals Sensitivity of winter rapeseed (Brassica napus L.) to isolates of the fungus Leptosphaeria maculans (Desm.) Ces. et de Not

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 572-574
Author(s):  
E. Plachká ◽  
L. Odstrčilová
Keyword(s):  
Plant Pathology ◽  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Brassica Napus L ◽  
Yellow Colour ◽  
Breeding Lines ◽  
University Of Wisconsin ◽  
Pure Cultures ◽  
Winter Rapeseed ◽  
And Control

The pure cultures of the fungus Leptosphaeria maculans were isolated from the rapeseed leaves collected in Moravia. Two isolates were collected on the basis of their ability to produce yellow colour. The isolate producing yellow colour was classified as aggressive, while the isolate without the ability to produce yellow colour was not aggressive. The sensitivity to these isolates was verified in 11 rapeseed genotypes. This test was based on the method from the document Crucifer Genetics Cooperative (information document from the Department of Plant Pathology, University of Wisconsin). This method is based on the inoculation of cruciferous leaflets with suspension from the isolate of the pathogen observed. The scale from 0 to 9 was used for evaluation (0 = no darkening around the wound, 9 = accompanied by profuse sporulation in large, more than 5 mm, lesions with diffuse margins). The infection degree of the aggressive isolate ranged in different genotypes from 1.45 to 4.5, the results of the non-aggressive isolate ranged from 1.0 to 1.5. Based on these results the aggressive isolate was used for further tests of the selected breeding lines. The tests involved selected breeding materials, registered varieties, control varieties with different sensitivity to the isolate of Leptosphaeria maculans and control varieties included in state trials. The maximal difference in the sensitivity exhibited by individual genotypes was five degrees.

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