Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis

2001 ◽  
Vol 102 (5) ◽  
pp. 700-708 ◽  
Author(s):  
P. A. Wiersma ◽  
Z. Wu ◽  
L. Zhou ◽  
C. Hampson ◽  
F. Kappel
2018 ◽  
Vol 15 (2) ◽  
pp. 150-158
Author(s):  
Ya. I. Ivanovych ◽  
N. V. Tryapitsyna ◽  
K. M. Udovychenko ◽  
R. A. Volkov

Aim. Ukrainian breeders have created a large number of sweet cherry cultivars, which still remain almost unexplored at the molecular level. The aim of our study was to identify the self-incompatibility alleles (S-alleles) in Ukrainian sweet cherry cultivars and landraces, and to elucidate, to which cross-incompatibility group the cultivars belong. Methods. The PCR was conducted using consensus primers to the first and second introns of S-RNAse gene and to the single intron of SFB gene. The electrophoretic analysis of the PCR products of the second intron of S-RNAse was carried out in agarose gel, whereas detection of fluorescently labeled DNA fragments of the first S-RNAse intron and the SFB intron was performed using a genetic analyzer. Results. The S-alleles of 25 Ukrainian sweet cherry cultivars and 10 landraces were identified. The S-alleles frequencies and affiliation of cultivars and landraces to the groups of cross-incompatibility were determined. The obtained data can be used in breeding programs and by planning of industrial plantings. Conclusions. In the study, 12 different S-alleles and 79 S-haplotypes were identified. The S1, S3, S4, S5, S6 and S9 alleles are the most widespread among Ukrainian sweet cherry cultivars and landraces. The high frequencies of S5 and especially of S9 alleles are characteristic for the Ukrainian cultivars and distinguish them from other European ones. For the Ukrainian sweet cherry cultivars, the XXXVII (S5S9) cross-incompatibility group appeared to be the most numerous.Keywords: Ukrainian sweet cherry cultivars, S-locus, Sgenotypes, self- and cross-incompatibility, Prunus avium.


Euphytica ◽  
2013 ◽  
Vol 191 (1) ◽  
pp. 153-164 ◽  
Author(s):  
A. Hegedűs ◽  
D. Taller ◽  
N. Papp ◽  
B. Szikriszt ◽  
S. Ercisli ◽  
...  

2021 ◽  
Vol 175 ◽  
pp. 111494
Author(s):  
Excequel Ponce ◽  
Blanca Alzola ◽  
Natalia Cáceres ◽  
Madeline Gas ◽  
Catalina Ferreira ◽  
...  

2008 ◽  
Vol 4 (4) ◽  
pp. 897-910 ◽  
Author(s):  
James W. Olmstead ◽  
Audrey M. Sebolt ◽  
Antonio Cabrera ◽  
Suneth S. Sooriyapathirana ◽  
Sue Hammar ◽  
...  

2017 ◽  
Vol 8 ◽  
Author(s):  
Xiliang Qi ◽  
Congli Liu ◽  
Lulu Song ◽  
Yuhong Li ◽  
Ming Li

Author(s):  
Michaela Benková ◽  
Iveta Čičová ◽  
Daniela Benedikova ◽  
Lubomir Mendel ◽  
Miroslav Glasa

Abstract The work is focused on the evaluation of variability of morphological and pomological characteristics of several old sweet cherries (Prunus avium L.) that were found in different Slovak regions. The experimental work has been performed during two years, 2014 and 2015. The following characteristics according to the descriptor list of subgenus Cerasus were evaluated - period of flowering and ripening, morphological characteristics of the flowers, fruit size, fruit weight, and quality parameters. The results showed high variability of evaluated accessions. From the 13 surveyed localities, the most valuable accessions were found in the locality Hornį Streda - places Čachtice, Krakovany, Nitra, and Brdárka. During the collecting expeditions, 170 accessions of sweet cherry, with fruit of the different quality were found. The most interesting accessions were grafted onto rootstocks with different intensity of growth (Prunus avium L., Prunus mahaleb L., and ‘Gisela5’). Some of the selected cherry accessions can be used for commercial growing after tests, while some of them can be used only for collection of genetic resources and as potential genitors in breeding programmes.


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