Fluctuation in recoverable nickel and zinc resistant copiotrophic bacteria explained by the varying zinc ion content of Torsa River in different months

IntroductionEpileptogenesis is understood as the plastic process that produces a persistent reorganization of the brain’s neural network after a precipitating injury (recurrent neonatal seizures, for instance) with a latent period, finally leading to neuronal hyperexcitability. Plasticity-related genes (PRGs), also known as lipid phosphate phosphatase-related proteins (PLPPRs), are regulators of mitochondrial membrane integrity and energy metabolism. This study was undertaken to determine whether PRG5 gene knockout contributes to the delayed hypersensitivity induced by developmental seizures and the aberrant sprouting of hippocampal mossy fibers, and to determine whether it is achieved through the mitochondrial pathway. Here, we developed a “twist” seizure model by coupling pilocarpine-induced juvenile seizures with later exposure to penicillin to test the long-term effects of PRG5 knockout on seizure latency through comparison with wild-type (WT) mice. Hippocampal mossy fiber sprouting (MFS) was detected by Timm staining. In order to clarify the mechanism of the adverse reactions triggered by PRG5 knockout, hippocampal HT22 neuronal cultures were exposed to glutamate, with or without PRG5 interference. Mitochondrial function, oxidative stress indicators and zinc ion content were detected.ResultsPRG5 gene knockout significantly reduced the seizure latency, and aggravated the lowered seizure threshold induced by developmental seizures. Besides, knockout of the PRG5 gene reduced the MFS scores to a certain extent. Furthermore, PRG5 gene silencing significantly increases the zinc ion content in hippocampal neurons, impairs neuronal activity and mitochondrial function, and exacerbates glutamate-induced oxidative stress damage.ConclusionIn summary, PRG5 KO is associated with significantly greater hypersusceptibility to juvenile seizures in PRG5(–/–) mice compared with WT mice. These effects may be related to the hippocampal zinc signaling. The effects do not appear to be related to changes in MFS because KO mice with juvenile seizures had the shortest seizure latencies but exhibited less MFS than WT mice with juvenile seizures.

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Temperature Dependence of the Self-Assembled Styrene Based Random Ionomers in Aqueous Solution

MRS Proceedings ◽

10.1557/proc-775-p6.27 ◽

2003 ◽

Vol 775 ◽

Author(s):

Sung-Hwa Oh ◽

Ju-Myung Song ◽

Joon-Seop Kim ◽

Hyang-Rim Oh ◽

Jeong-A Yu

Keyword(s):

Partition Coefficients ◽

Room Temperature ◽

Aqueous Media ◽

The Self ◽

Spectroscopic Methods ◽

Ion Content ◽

Repeat Units ◽

Notable Effect ◽

Order Of Magnitude ◽

Experimental Errors

AbstractSolution behaviors of poly(styrene-co-sodium methacrylate) were studied by fluorescence spectroscopic methods using pyrene as a probe. The mol% of methacrylate was in the range 3.6–9.4. Water and N,N-dimethylforamide(DMF) mixture was used as a solvent (DMF/water = 0.2 mol %). The critical micelle (or aggregation) concentrations of ionomers and the partition coefficients of pyrene were obtained the temperature range 10–80°C. At room temperature, the values of CMCs (or CACs) were in the range 4.7 ×10-6 5.3 ×10-6 g/mL and we could not find any notable effect of the content of ionic repeat units within the experimental errors. Unlike CMCs, as the ion content increased, partitioning of pyrene between the hydrophobic aggregates and an aqueous media decreased from 1.5 ×105 to 9.4 ×104. As the temperature increased from 10 to 80 °C, the values of CMCs increased less than one order of magnitude. While, the partition coefficients of pyrene decreased one order of magnitude and the effect of the ion content became negligible.

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Insulinlike effects of zinc ion in vitro and in vivo. Preferential effects on desensitized adipocytes and induction of normoglycemia in streptozocin-induced rats

Diabetes ◽

10.2337/diabetes.41.8.982 ◽

1992 ◽

Vol 41 (8) ◽

pp. 982-988 ◽

Cited By ~ 21

Author(s):

A. Shisheva ◽

D. Gefel ◽

Y. Shechter

Keyword(s):

Zinc Ion

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Matrix metalloproteinases: structure, functions and genetic polymorphism

Nauchno-prakticheskii zhurnal «Patogenez» ◽

10.25557/gm.2017.2.7297 ◽

2017 ◽

pp. 14-23

Author(s):

А.С. Шадрина ◽

И.В. Терешкина ◽

Я.З. Плиева ◽

Д.Н. Кушлинский ◽

Д.О. Уткин ◽

...

Keyword(s):

Genetic Polymorphism ◽

Matrix Metalloproteinases ◽

Active Center ◽

Human Body ◽

Enzymatic Catalysis ◽

Structure Functions ◽

Zinc Ion ◽

Etiological Factors ◽

Mmp Genes

Матриксные металлопротеиназы (ММП) - ферменты класса гидролаз, осуществляющие ферментативный катализ с помощью связанного в активном центре иона цинка. Функции ММП разнообразны, и нарушение баланса их активности может быть одним из этиологических факторов различных заболеваний. В данном обзоре рассмотрена классификация ММП человека, особенности их структуры и регуляции, а также роль в физиологических и патологических процессах в организме человека. Приведен перечень наиболее изученных на настоящий момент полиморфных вариантов генов MMП, описаны их функциональные эффекты и представлены результаты ассоциативных исследований. Matrix metalloproteinases (MMPs) are enzymes of the hydrolase class that carry out enzymatic catalysis with the help of a zinc ion bound in the active center. MMP functions are diverse, and a disturbance in the balance of their activity may be one of the etiological factors of various diseases. In this review, the classification of human MMP, the features of their structure and regulation, as well as the role in physiological and pathological processes in the human body are considered. A list of the most studied polymorphic versions of MMP genes has been given, their functional effects have been described, and the results of associative studies have been presented.

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1285 - Minimal concentrations of zinc ion promoted conjugative transfer of kanamycin resistance gene mediated by a plasmid isolated from chicken manure

10.26226/morressier.5b5199c2b1b87b000ecee381 ◽

2018 ◽

Author(s):

Chengjun Pu

Keyword(s):

Resistance Gene ◽

Zinc Ion ◽

Kanamycin Resistance ◽

Chicken Manure ◽

Conjugative Transfer ◽

Kanamycin Resistance Gene

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Restriction of Access to Dark State: A New Mechanistic Model for Heteroatom-Containing AIE Systems

10.26434/chemrxiv.8275325.v1 ◽

2019 ◽

Author(s):

Yujie Tu ◽

Junkai Liu ◽

Haoke Zhang ◽

Qian Peng ◽

Jacky W. Y. Lam ◽

...

Keyword(s):

Excited States ◽

Radiative Decay ◽

Molecular Design ◽

Mechanistic Model ◽

Zinc Ion ◽

Easy Access ◽

Triplet States ◽

Dark State ◽

Ion Probe ◽

Π State

Aggregation-induced emission (AIE) is an unusual photophysical phenomenon and provides an effective and advantageous strategy for the design of highly emissive materials in versatile applications such as sensing, imaging, and theragnosis. "Restriction of intramolecular motion" is the well-recognized working mechanism of AIE and have guided the molecular design of most AIE materials. However, it sometimes fails to be workable to some heteroatom-containing systems. Herein, in this work, we take more than one excited state into account and specify a mechanism –"restriction of access to dark state (RADS)" – to explain the AIE effect of heteroatom-containing molecules. An anthracene-based zinc ion probe named APA is chosen as the model compound, whose weak fluorescence in solution is ascribed to the easy access from the bright (π,π*) state to the closelying dark (n,π*) state caused by the strong vibronic coupling of the two excited states. By either metal complexation or aggregation, the dark state is less accessible due to the restriction of the molecular motion leading to the dark state and elevation of the dark state energy, thus the emission of the bright state is restored. RADS is found to be powerful in elucidating the photophysics of AIE materials with excited states which favor non-radiative decay, including overlap-forbidden states such as (n,π*) and CT states, spin-forbidden triplet states, which commonly exist in heteroatom-containing molecules.

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