Microbial consortium composed of Cellulomonas ZJW-6 and Acinetobacter DA-25 improves straw lignocellulose degradation

2022 ◽  
Vol 204 (2) ◽  
Author(s):  
Yunpeng Guan ◽  
Hongyu Zhu ◽  
Yuan Zhu ◽  
Hemei Zhao ◽  
Longhua Shu ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Lignocellulose Degradation

Isolation and Characterization of a Novel Laccase LacZ1 for Lignin Degradation

2021 ◽  
Author(s):  
Weiran Zhang ◽  
Weiwei Wang ◽  
Jinghong Wang ◽  
Guinan Shen ◽  
Yuan Yuan ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
High Temperature ◽  
Lignin Degradation ◽  
Microbial Consortium ◽  
Organic Polymer ◽  
Lignocellulose Degradation ◽  
Bacterial Laccase ◽  
Isolation And Characterization ◽  
Acid Pathway ◽  
Insight Into

Lignin is a complex natural organic polymer and is one of the primary components of lignocellulose. The efficient utilization of lignocellulose is limited because it is difficult to degrade lignin. In this study, we screened a lacz1 gene fragment encoding laccase from the macro transcriptome data of a microbial consortium WSC-6, which can efficiently degrade lignocellulose. The RT-qPCR results demonstrated that the expression level of the lacz1 gene during the peak period of lignocellulose degradation by WSC-6 increased by 30.63 times compared to the initial degradation period. Phylogenetic tree analysis demonstrated that the complete lacz1 gene is derived from Bacillus sp. and encoded laccase. The corresponding protein LacZ1 was expressed and purified by Ni-chelating affinity chromatography. The optimum temperature was 75°C, the optimum pH was 4.5, and the highest enzyme activity reached 16.39 U/mg. We found that Cu 2+ was an important cofactor needed for LacZ1 to have enzyme activity. The molecular weight distribution of lignin was determined by Gel Permeation Chromatography (GPC) and changes in the lignin structure were determined by 1H Nuclear Magnetic Resonance Spectra (1H NMR). The degradation products of lignin by LacZ1 were determined by Gas Chromatography and Mass Spectrometry (GC-MS), and three lignin degradation pathways (the gentian acid pathway, benzoic acid pathway, and protocatechuic acid pathway) were proposed. This study provides insight into the degradation of lignin and new insights into high-temperature bacterial laccase. IMPORTANCE Lignin is a natural aromatic polymer that is not easily degraded, hindering the efficient use of lignocellulose-rich biomass resources, such as straw. Biodegradation is a method of decomposing lignin that has recently received increasing attention. In this study, we screened a gene encoding laccase from the lignocellulose-degrading microbial consortium WSC-6, purified the corresponding protein LacZ1, characterized the enzymatic properties of laccase LacZ1, and speculated that the degradation pathway of LacZ1 degrades lignin. This study identified a new, high-temperature bacterial laccase that can degrade lignin, providing insight into lignin degradation by this laccase.

scholarly journals Metagenomic insight into lignocellulose degradation of the thermophilic microbial consortium TMC7

2021 ◽  
Author(s):  
Yi Wang ◽  
Chen Wang ◽  
Yonglun Chen ◽  
Beibei Chen ◽  
Peng Guo ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Lignocellulose Degradation ◽  
Insight Into

scholarly journals Microbial consortium OEM1 cultivation for higher lignocellulose degradation and chlorophenol removal

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 39011-39017 ◽  
Author(s):  
Jiajin Liang ◽  
Yunqin Lin ◽  
Tian Li ◽  
Fanyong Mo
Keyword(s):  
Microbial Consortium ◽  
Lignocellulose Degradation

Herein, microbial consortium OEM1 that could simultaneously decompose lignocellulose and chlorophenols was screened from spent mushroom substrates.

New combination of xylanolytic bacteria isolated from the lignocellulose degradation microbial consortium XDC-2 with enhanced xylanase activity

2016 ◽  
Vol 221 ◽  
pp. 686-690 ◽  
Author(s):  
Dongdong Zhang ◽  
Yi Wang ◽  
Dan Zheng ◽  
Peng Guo ◽  
Wei Cheng ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Xylanase Activity ◽  
New Combination ◽  
Lignocellulose Degradation

scholarly journals Significant Improvement of Straw  Lignocellulose Degradation Rate by Subordination Consortium Composed of Cellulomonas ZJW-6 and Acinetobacter DA-25

2021 ◽  
Author(s):  
Yunpeng Guan ◽  
Hongyu Zhu ◽  
Yuan Zhu ◽  
Longhua Shu ◽  
Hemei Zhao ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Agricultural Development ◽  
Degradation Rate ◽  
High Efficiency ◽  
Microbial Consortium ◽  
Temperature Tolerance ◽  
Degradation Efficiency ◽  
Microbial Consortia ◽  
Lignocellulose Degradation ◽  
Glucosidase Activity

Abstract BackgroundThe effective utilization of lignocellulose is essential for achieving circular economy and sustainable agricultural development. The screening of lignocellulose-degrading strains from the environment and artificial establishment of microbial consortia can achieve better degradation effects. In this paper, microbial consortium 625 was established and screened based on high lignocellulose degradation ability but low β-glucosidase activity of Cellulomonas ZJW-6 to complement enzyme activity and enhance lignocellulose degradation by exerting the subordination effect. This research provides an effective strategy for artificially establishing microbial consortia, improving lignocellulose degradation efficiency, and enhancing bioenergy production.ResultsIn order to establish high-efficiency microbial consortia, Cellulomonas ZJW-6 with low β-glucosidase activity was combined in pairs with 14 strains with high β-glucosidase activity according to the enzyme activity complementation principle, from which microbial consortium 625 composed of strains ZJW-6 and DA-25 was screened, which had significantly enhanced lignocellulose degradation rate compared with purely cultured ZJW-6 strain and other 13 combinations. The single factor test showed that consortium 625 had better pH and temperature tolerance. The lignocellulose degradation rate of this consortium was 57.62% after verification test under optimized conditions using response surface method. The induction test showed that the growth of strain DA-25 was inhibited by strain ZJW-6 and its filtrate, while strain DA-25 and its filtrate promoted the growth of strain ZJW-6 and significantly enhanced the activities of key enzymes in the consortium 625 lignocellulose degrading system, among which the β-Glucosidase activity was significantly higher than that in purely cultured ZJW-6 strain. ConclusionMicrobial consortium 625, composed of Cellulomonas ZJW-6 (dominant strain) and Acinetobacter DA-25, with high lignocellulose degradation efficiency, was screened according to the enzyme activity complementation principle. Interestingly, microbial consortium 625 exhibited complementation in enzyme activities of its two strains, improvement in its tolerance to temperature and pH, and the same effect of DA-25 filtrate as strain DA-25. Based on the previous studies on Acinetobacter, we speculate that a certain compound adverse to the growth of strain DA-25 may be produced during lignocellulose degradation by strain ZJW-6, while secretions of strain DA-25 inhibit this compound leading to an increase in the growth and enzyme activity of strain ZJW-6.

Characteristics and selection of efficient lignocellulose degradation microbial community

2013 ◽  
Vol 21 (5) ◽  
pp. 621-627
Author(s):  
De-Wu WANG ◽  
Tuo YAO ◽  
Qiao-Li YANG ◽  
Guo-Tao QI ◽  
Xin-Yi LIU ◽  
...  
Keyword(s):  
Microbial Community ◽  
Lignocellulose Degradation ◽  
Selection Of

Effect of Solids Retention Time on Nitrogen Removal and Microbial Consortium in a Novel Algal-Bacterial Shortcut Nitrogen Removal System

2017 ◽  
Vol 2017 (3) ◽  
pp. 225-230
Author(s):  
Nadezhda Zalivina ◽  
Ryan Keeley ◽  
Larissa T Arashiro ◽  
Angelica Rada-Ariza ◽  
Meng Wang ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Retention Time ◽  
Microbial Consortium ◽  
Solids Retention Time ◽  
Solids Retention ◽  
Shortcut Nitrogen Removal ◽  
Removal System

Production of 1,3-propanediol and lactic acid from crude glycerol by a microbial consortium from intertidal sludge

2021 ◽  
Author(s):  
Li-Li Jiang ◽  
Feng-Yi Liu ◽  
Wei Yang ◽  
Chang-Li Li ◽  
Bao-Wei Zhu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Crude Glycerol ◽  
Microbial Consortium
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