Miniaturized fiber-in-tube solid-phase extraction as the sample preconcentration method for microcolumn liquid-phase separations

2001 ◽  
Vol 372 (1) ◽  
pp. 164-168 ◽  
Author(s):  
Yoshihiro Saito ◽  
Motohiro Imaizumi ◽  
Tsutomu Takeichi ◽  
Kiyokatsu Jinno
Keyword(s):  
Liquid Phase ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Phase Separations ◽  
Sample Preconcentration ◽  
Liquid Phase Separations ◽  
Preconcentration Method

scholarly journals Modern Approaches to Preparation of Body Fluids for Determination of Bioactive Compounds

Separations ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 53 ◽  
Author(s):  
Katarzyna Madej ◽  
Wojciech Piekoszewski
Keyword(s):  
Liquid Phase ◽  
Solid Phase Extraction ◽  
Bioactive Compounds ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Extraction Methods ◽  
Body Fluids ◽  
Phase Extraction ◽  
Modern Approach ◽  
Dispersive Liquid Liquid Microextraction

The current clinical and forensic toxicological analysis of body fluids requires a modern approach to sample preparation characterized by high selectivity and enrichment capability, suitability for micro-samples, simplicity and speed, and the possibility of automation and miniaturization, as well as the use of small amounts of reagents, especially toxic solvents. Most of the abovementioned features may be realized using so-called microextraction techniques which cover liquid-phase techniques (e.g., single-drop microextraction, SDME; dispersive liquid–liquid microextraction, DLLME; hollow-fiber liquid-phase microextraction, HF-LPME) and solid-phase extraction techniques (solid-phase microextraction, SPME; microextraction in packed syringes, MEPS; disposable pipette tip extraction, DPX; stir bar sorption extraction, SBSE). Some other extraction methodologies like dispersive solid-phase extraction (d-SPE) or magnetic solid-phase extraction (MSPE) can also be easily miniaturized. This review briefly describes and characterizes the abovementioned extraction methods, and then presents their current applications to the preparation of body fluids analyzed for bioactive compounds in combination with appropriate analytical methods, mainly chromatographic and related techniques. The perspectives of the analytical area we are interested in are also indicated.

scholarly journals A novel method for liquid-phase extraction of cell-free DNA for detection of circulating tumor DNA

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Filip Janku ◽  
Helen J. Huang ◽  
David Y. Pereira ◽  
Masae Kobayashi ◽  
Chung Hei Chiu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Solid Phase Extraction ◽  
Extraction Method ◽  
Solid Phase ◽  
Cancer Diagnostics ◽  
Phase Extraction ◽  
Plasma Samples ◽  
Cell Free Dna ◽  
Dna Yield ◽  
Free Dna

AbstractLow yields of extracted cell-free DNA (cfDNA) from plasma limit continued development of liquid biopsy in cancer, especially in early-stage cancer diagnostics and cancer screening applications. We investigate a novel liquid-phase-based DNA isolation method that utilizes aqueous two-phase systems to purify and concentrate circulating cfDNA. The PHASIFY MAX and PHASIFY ENRICH kits were compared to a commonly employed solid-phase extraction method on their ability to extract cfDNA from a set of 91 frozen plasma samples from cancer patients. Droplet digital PCR (ddPCR) was used as the downstream diagnostic to detect mutant copies. Compared to the QIAamp Circulating Nucleic Acid (QCNA) kit, the PHASIFY MAX method demonstrated 60% increase in DNA yield and 171% increase in mutant copy recovery, and the PHASIFY ENRICH kit demonstrated a 35% decrease in DNA yield with a 153% increase in mutant copy recovery. A follow-up study with PHASIFY ENRICH resulted in the positive conversion of 9 out of 47 plasma samples previously determined negative with QCNA extraction (all with known positive tissue genotyping). Our results indicate that this novel extraction technique offers higher cfDNA recovery resulting in better sensitivity for detection of cfDNA mutations compared to a commonly used solid-phase extraction method.

scholarly journals Ionic liquid functionlized on multiwall carbon nanotubes for nickel and lead determination in human serum and urine samples by micro solid-phase extraction

2021 ◽  
Vol 4 (02) ◽  
pp. 72-85
Author(s):  
Arezou Lari ◽  
Nafiseh Esmaeili ◽  
Homanaz Ghafari
Keyword(s):  
Carbon Nanotubes ◽  
Ionic Liquid ◽  
Liquid Phase ◽  
Solid Phase Extraction ◽  
Human Serum ◽  
Solid Phase ◽  
Multiwall Carbon Nanotubes ◽  
Urine Samples ◽  
Phase Extraction ◽  
Multiwall Carbon

In this study, a novel synthesis adsorbent, 1-(3-aminopropyl)-3-methylimidazolium hexafluorophosphate functionlized on multiwall carbon nanotubes ([Apmim][PF6]-MWCNTs, IL@MWCNTS) was used for nickel/lead (Ni/Pb) extraction and determination by dispersive ionic liquid micro solid-phase extraction (DIL-μ-SPE) coupled to electrothermal atomic absorption spectrometry (ET-AAS). After dilution of 20 mg of IL@MWCNTS in 200 μL of acetone, the mixture was injected to 10 mL of human serum/urine samples at pH of 8.0. After sonication for 5 min, the Ni(II) / Pb(II) were extracted by ionic liquid phase and then centrifuged for 2.5 min. The upper liquid phase set aside and Ni(II) / Pb(II) loaded in adsorbent were back-extracted by acidic solution at pH=2-3. Finally, the concentration of total nickel and lead was determined by ET-AAS. By optimizing, the limit of detection, linear range, and enrichment factor for nickel and lead were obtained (0.05 μg L−1; 0.1 μg L−1), (0.2-5.8 μg L−1; 0.4-30 μg L−1) and 24.7; 5.1, respectively (RSD less than 5%). Also, the capacity absorption of IL@MWCNTS for nickel and lead ions were achieved 149.3 mg g-1 and 162.5 mg g-1, respectively.  The DIL-μ-SPE procedure was validated for nickel and lead extraction by spiking of real samples and ICP-MS analyzer.

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