Structural characterization of multiple pyoverdines secreted by two Pseudomonas strains using liquid chromatography-high resolution tandem mass spectrometry with varying dissociation energies

2015 ◽  
Vol 407 (16) ◽  
pp. 4629-4638 ◽  
Author(s):  
Hua Wei ◽  
Ludmilla Aristilde
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Tandem Mass ◽  
Dissociation Energies

Identification and structural characterization of four novel degradation products and a process impurity of isavuconazonium sulfate for injection formulation bulk by LC-ESI-QTOF-MS/MS

2019 ◽  
Vol 26 (2) ◽  
pp. 144-152
Author(s):  
Venkata Rama Prabhakara Sastry Regella ◽  
Venkatesan Chidambaram Subramanian ◽  
Sarveswara Sastry Bhetanabhotla
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Alkaline Hydrolysis ◽  
Degradation Products ◽  
Water Soluble ◽  
Tandem Mass ◽  
Light Conditions

Isavuconazonium sulfate is a highly water-soluble pro-drug of active triazole isavuconazole. The degradation profile of isavuconazonium sulfate for injection formulation bulk under acid hydrolysis, alkaline hydrolysis, oxidation, heat, and light conditions was studied using LC-PDA/MS technique. Four degradation products (DP1 to DP4) and one process impurity (PI1) were identified in isavuconazonium sulfate formulation bulk and characterized the identified impurities by liquid chromatography-electron spray ionization-quadruple-time of flight tandem mass spectrometry. Isavuconazole (DP1) was found to be one of the major degradation products of isavuconazonium sulfate.

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