Effect of extraction pH on metal speciation in plant root extracts

The free amino acids and the total ninhydrin-reacting material found in the rhizosphere of plants grown under field conditions were compared with those in the nonrhizosphere soil and with root extracts. Water, 20% ethanol and carbon tetrachloride-water were used as extractants. While the last two removed more amino acids than the water, this was probably the result of partial decomposition of the cellular material of the soil. The use of water as an extractant should give a better measure of the amount of amino acids in the soil under natural conditions. The rhizosphere contained greater quantities of amino acids than the soil from outside this zone. In general, the most prominent amino acids were aspartic acid, threonine, serine, glutamic acid, glycine and alanine; asparagine, glutamine and citrulline were also present. The amino acid content of the rhizosphere varied with the plant. Although root extracts contained very large quantities of amino acids, comparison of these extracts with the rhizosphere showed distinct differences in amino acid ratios, suggesting that the amino acids in the rhizosphere were not primarily the result of root-debris autolysis during the extraction procedure.

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Benzo[a]pyrene co-metabolism in the presence of plant root extracts and exudates: Implications for phytoremediation

Environmental Pollution ◽

10.1016/j.envpol.2004.12.034 ◽

2005 ◽

Vol 136 (3) ◽

pp. 477-484 ◽

Cited By ~ 124

Author(s):

Jeremy A. Rentz ◽

Pedro J.J. Alvarez ◽

Jerald L. Schnoor

Keyword(s):

Plant Root ◽

Root Extracts

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Aldrin epoxidation by plant root extracts

Phytochemistry ◽

10.1016/0031-9422(73)80373-5 ◽

1973 ◽

Vol 12 (7) ◽

pp. 1591-1594 ◽

Cited By ~ 7

Author(s):

Harihara M. Mehendale

Keyword(s):

Plant Root ◽

Root Extracts

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Evaluation of anxiolytic potential of Cichorium Intybus methanolic root extracts

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v9i6.817 ◽

2020 ◽

Vol 9 (6) ◽

pp. 25-30

Author(s):

S.Tripathy ◽

N.K.S Neeraja

Keyword(s):

Animal Models ◽

Motor Coordination ◽

Elevated Plus Maze ◽

Plant Root ◽

Cichorium Intybus ◽

Significant Alteration ◽

Anticipatory Anxiety ◽

Root Extracts ◽

Plus Maze ◽

The Family

Cichorium Intybus L. is a medicinally important plant that belongs to the family Asteraceae. The plant root is used as anti-hepatotoxic, antiulcerogenic, antiinflammatory, appetizer, digestive, stomachic, liver tonic, cholagogue, cardiotonic, depurative, diuretic, emmenagogue, febrifuge, alexeteric and also as tonic. The present study was carried to screen the anxiolytic potential of Cichorium Intybus L methanolic root extracts by using different animal models like actophotometer, rotarod, staircase, holeboard, elevated plus maze tests . In the experiment the extracts found to decrease the anticipatory anxiety of the animal without significant alteration in motor coordination. Efficacy may be attributed to the presence of different phytochemicals like coumarins, flavonoids in the extracts.

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Repression of Pseudomonas putida phenanthrene-degrading activity by plant root extracts and exudates

Environmental Microbiology ◽

10.1111/j.1462-2920.2004.00589.x ◽

2004 ◽

Vol 6 (6) ◽

pp. 574-583 ◽

Cited By ~ 103

Author(s):

Jeremy A. Rentz ◽

Pedro J. J. Alvarez ◽

Jerald L. Schnoor

Keyword(s):

Pseudomonas Putida ◽

Plant Root ◽

Root Extracts

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Aldicarb sulfoxidation by plant root extracts

Pesticide Biochemistry and Physiology ◽

10.1016/0048-3575(77)90027-x ◽

1977 ◽

Vol 7 (2) ◽

pp. 154-160 ◽

Cited By ~ 10

Author(s):

H.R. Krueger

Keyword(s):

Plant Root ◽

Root Extracts

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Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots ofRhaponticum carthamoidesagainst Induced Oxidative Stress and DNA Damage in CHO Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/5753139 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

Ewa Skała ◽

Przemysław Sitarek ◽

Marek Różalski ◽

Urszula Krajewska ◽

Janusz Szemraj ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Dna Repair ◽

Cho Cells ◽

Plant Root ◽

Antioxidant Properties ◽

Chinese Hamster ◽

Root Extract ◽

Root Extracts ◽

Grown Plant

Rhaponticum carthamoideshas a long tradition of use in Siberian folk medicine. The roots and rhizomes of this species are used in various dietary supplements or nutraceutical preparations to increase energy level or eliminate physical weakness. This is the first report to reveal the protective and DNA repair stimulating abilities ofR. carthamoidesroot extracts in Chinese hamster ovary (CHO) cells exposed to an oxidative agent. Both transformed root extract (TR extract) and extract of soil-grown plant roots (NR extract) may be responsible for stimulating CHO cells to repair oxidatively induced DNA damage, but CHO cells stimulated with extract from the transformed roots demonstrated significantly stronger properties than cells treated with the soil-grown plant root extract. These differences in biological activity may be attributed to the differences in the content of phenolic compounds in these root extracts. Preincubation of the CHO cells with TR and NR extracts showed an increase in gene expression and protein levels of catalase (CAT) and superoxide dismutase (SOD2).R. carthamoidesmay possess antioxidant properties that protect CHO cells against oxidative stress.

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Behavioural differences of Heterodera glycines and Meloidogyne incognita infective juveniles exposed to root extracts in vitro

Nematology ◽

10.1163/15685411-00003038 ◽

2017 ◽

Vol 19 (2) ◽

pp. 175-183 ◽

Cited By ~ 4

Author(s):

Edward P. Masler ◽

Stephen T. Rogers ◽

Cerruti R. R. Hooks

Keyword(s):

Meloidogyne Incognita ◽

Heterodera Glycines ◽

Plant Root ◽

Agar Plate ◽

Root Extract ◽

Tagetes Patula ◽

Water Control ◽

Root Extracts ◽

Agar Disc

In vitro behaviour of infective second-stage juveniles (J2) of Heterodera glycines and Meloidogyne incognita was compared in the presence and absence of plant root extracts. In an agar plate attraction-retention assay, with samples applied by agar disc infused with water (control) or aqueous test solutions, H. glycines was 15-fold more responsive to a chemical attractant (CaCl2) than was M. incognita. Control discs retained H. glycines at a rate 2.9-fold greater than M. incognita. Crude extracts (slurries; 40 mg dry root (ml water)−1) from roots of six plant species (corn, Zea mays; cucumber, Cucumis sativus; marigold, Tagetes patula; mustard, Sinapis alba; pepper, Capsicum annuum; soybean, Glycine max) differentially affected the two nematodes. Cucumber, marigold, pepper and soybean each attracted H. glycines at rates between 2.2- and 3.6-fold greater than controls. No root preparations were attractive to M. incognita, which were significantly repelled by corn, cucumber, mustard and pepper, relative to controls. Preparation of selected root extract supernatants, which involved vacuum drying, decreased the attractiveness of marigold and soybean to H. glycines by 38 and 82%, respectively, but the effect of pepper was unchanged. Supernatant processing had no effect on M. incognita behaviour. In a liquid-based J2 movement assay, root supernatants from marigold, pepper and soybean at 1 mg dry root ml−1 each decreased the frequency of head movement in H. glycines and M. incognita relative to controls. However, dose responses were detected only with marigold, with maximum decreases in activity at 16 mg dry root ml−1 for each species. These decreases were significantly different at 46 and 66%, respectively, for H. glycines and M. incognita. The behaviour of the two nematodes was qualitatively different in assays that required detection of signals across a short distance (agar assay), whereas qualitative responses were similar when juveniles were immersed in treatment solution (liquid assay). In the latter, quantitative responses to marigold differed significantly between H. glycines and M. incognita J2.

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