L-Carnitine and Isovaleryl L-Carnitine Fumarate Positively Affect Human Osteoblast Proliferation and Differentiation In Vitro

Purpose: In girls and women, the authors studied the effects of an acute bout of low-impact, moderate-intensity exercise serum on myoblast and osteoblast proliferation in vitro. Methods: A total of 12 pre/early pubertal girls (8–10 y old) and 12 women (20–30 y old) cycled at 60% VO2max for 1 hour followed by 1-hour recovery. Blood samples were collected at rest, mid-exercise, end of exercise, mid-recovery, and end of recovery. C2C12 myoblasts and MC3T3E1 osteoblasts were incubated with serum from each time point for 1 hour, then monitored for 24 hours (myoblasts) or 36 hours (osteoblasts) to examine proliferation. Cells were also monitored for 6 days (myoblasts) to examine myotube formation and 21 days (osteoblasts) to examine mineralization. Results: Exercise did not affect myoblast or osteoblast proliferation. Girls exhibited lower cell proliferation relative to women at end of exercise (osteoblasts, P = .041; myoblasts, P = .029) and mid-recovery (osteoblasts, P = .010). Mineralization was lower at end of recovery relative to rest (P = .014) in both girls and women. Myotube formation was not affected by exercise or group. Conclusion: The systemic environment following one acute bout of low-impact moderate-intensity exercise in girls and women does not elicit osteoblast or myoblast activity in vitro. Differences in myoblast and osteoblast proliferation between girls and women may be influenced by maturation.

Download Full-text

Biomimetic Mineralization and Bioactivity of Phosphorylated Chitosan

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.288-289.429 ◽

2005 ◽

Vol 288-289 ◽

pp. 429-432 ◽

Cited By ~ 10

Author(s):

Zhi Qing Chen ◽

Quan Li Li ◽

Quan Zen ◽

Gang Li ◽

Hao Bin Jiang ◽

...

Keyword(s):

Calcium Phosphate ◽

Simulated Body Fluid ◽

Body Fluid ◽

Crystal Nucleation ◽

Osteoblast Proliferation ◽

Biomimetic Mineralization ◽

P Content ◽

Proliferation And Differentiation ◽

Nucleation Growth

Phosphorylated chitosans were synthesized as templates to manipulate hydroxyapatite (HA) crystal nucleation, growth and microstructure. Two kinds of insoluble phosphorylated chitosan were soaked in saturated Ca(OH)2 solution for 4 d and in 1.5× SBF (simulated body fluid) solutions for 14 d at 37 °C for biomimetic mineralization. A lower [P]-content of phosphorylated chitosan promoted greater mineralization than higher [P]-content. Phosphorylated chitosan inhibited osteoblast proliferation and differentiation in vitro, while calcium phosphate phosphorylated chitosan composites did not.

Download Full-text

Mesoporous Bioactive Glass Promoted Osteoblast Proliferation and Differentiation In Vitro

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2019.2007 ◽

2019 ◽

Vol 9 (4) ◽

pp. 462-467

Author(s):

Jianwei Chen ◽

Xiaosheng Yu ◽

Hao Ji ◽

Zhen Zong ◽

Wei Hong ◽

...

Keyword(s):

Bioactive Glass ◽

Osteoblast Proliferation ◽

Proliferation And Differentiation ◽

Mesoporous Bioactive Glass

Download Full-text

Effect of Titanium Nanoparticles on Osteoblast Proliferation

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2020.3126 ◽

2020 ◽

Vol 12 (4) ◽

pp. 455-460

Author(s):

Yuan Wu ◽

Cuizhong Liu ◽

Ming Gao ◽

Qiang Liang ◽

Yu Jiang

Keyword(s):

Histochemical Staining ◽

Control Group ◽

Osteoblast Proliferation ◽

Osteogenic Activity ◽

Diphenyltetrazolium Bromide ◽

Proliferation And Differentiation ◽

Titanium Nanoparticles

This study aimed to observe the effect of titanium nanomaterials on osteoblastsin vitro. Osteoblasts were identified using histochemical staining, and they were examined using an MTT (3-(4,5Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to determine the proliferation and differentiation of osteoblasts. In addition, we observed the effect of titanium nanomaterials on the function of osteoblasts. Compared with the control group, titanium nanomaterials promoted the growth, proliferation, and differentiation of osteoblasts. Our findings showed that titanium nanomaterials can significantly promote the proliferation of osteoblasts and enhance their osteogenic activity.

Download Full-text