A polymerase chain reaction-based test for Verticillium fungicola causing dry bubble disease on the cultivated mushroom, Agaricus bisporus

2002 ◽  
Vol 59 (6) ◽  
pp. 695-699 ◽  
Author(s):  
C. P. Romaine ◽  
B. Schlagnhaufer ◽  
M. Stone
Keyword(s):  
Polymerase Chain Reaction ◽  
Agaricus Bisporus ◽  
Chain Reaction ◽  
Verticillium Fungicola ◽  
Dry Bubble ◽  
Cultivated Mushroom ◽  
Polymerase Chain ◽  
Dry Bubble Disease

scholarly journals BseGI Restriction of the Polymerase Chain Reaction Amplicon Th444 Is Required to Distinguish Biotypes of Trichoderma aggressivum Causing Serious Losses in Mushroom (Agaricus bisporus) Production

HortScience ◽  
2010 ◽  
Vol 45 (12) ◽  
pp. 1910-1911
Author(s):  
Mirosława Staniaszek ◽  
Katarzyna Szajko ◽  
Zbigniew Uliński ◽  
Magdalena Szczech ◽  
Waldemar Marczewski
Keyword(s):  
Polymerase Chain Reaction ◽  
Agaricus Bisporus ◽  
Polymerase Chain Reaction Amplicon ◽  
Chain Reaction ◽  
Restriction Fragments ◽  
Green Mold ◽  
Cultivated Mushroom ◽  
Polymerase Chain ◽  
Serious Disease ◽  
Reference Strains

Green mold is a serious disease of the cultivated mushroom causing losses in production of economical importance. In the present study, digestion of a Th444 amplicon with endonuclease BseGI was useful to discriminate Trichoderma aggressivum f. aggressivum (T.a.f.a) from the T. aggressivum f. europeanum (T.a.f.e.). The informative restriction fragments of 260 and 300 bp were revealed in the corresponding reference strains T.a.f.a. and T.a.f.e. The 300-bp marker was found in all 28 Polish mushroom isolates tested.

Genetic and physiological variation in isolates of Verticillium fungicola causing dry bubble disease of the cultivated button mushroom, Agaricus bisporus

2002 ◽  
Vol 106 (10) ◽  
pp. 1163-1170 ◽  
Author(s):  
Sergio Juarez Del Carmen ◽  
Michele L. Largeteau-Mamoun ◽  
Thierry Rousseau ◽  
Catherine Regnault-Roger ◽  
Jean-Michel Savoie
Keyword(s):  
Agaricus Bisporus ◽  
Button Mushroom ◽  
Physiological Variation ◽  
Verticillium Fungicola ◽  
Dry Bubble ◽  
Dry Bubble Disease

Verticillium disease or "dry bubble" of cultivated mushrooms: the Agaricus bisporus lectin recognizes and binds the Verticillium fungicola cell wall glucogalactomannan

2004 ◽  
Vol 50 (9) ◽  
pp. 729-735 ◽  
Author(s):  
Dolores Bernardo ◽  
Amelia Pérez Cabo ◽  
Monique Novaes-Ledieu ◽  
Concepción García Mendoza
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Agaricus Bisporus ◽  
Immunofluorescence Microscopy ◽  
Fruit Body ◽  
Vegetative Mycelium ◽  
Verticillium Fungicola ◽  
Dry Bubble ◽  
Cultivated Mushroom ◽  
Cultivated Mushrooms

The step of recognition and (or) binding for the development of the disease of the cultivated mushroom Agaricus bisporus by the mycoparasite Verticillium fungicola was studied by several approaches: agglutination of V. fungicola germinated spores by an A. bisporus extract from fruit body cell walls, immunofluorescence microscopy of A. bisporus hyphae from fruit bodies and vegetative mycelia pretreated with purified V. fungicola cell wall glucogalactomannan, and finally, by hemagglutination experiments carried out with an A. bisporus fruit body lectin in the presence and absence of the same glucogalactomannan. Hemagglutinating activity of the purified A. bisporus fruit body lectin was clearly inhibited by the V. fungicola glucogalactomannan, whereas in the A. bisporus vegetative mycelium such lectin was not encountered. All the results obtained make evident the recognition and binding of the A. bisporus fruit body lectin to the V. fungicola cell wall glucogalactomannan, clarifying why the mushrooms, but not the vegetative mycelium, become diseased.Key words: Agaricus bisporus lectin, Verticillium fungicola glucogalactomannan, mycoparasitism.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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