Autodisplay of nitrilase from Klebsiella pneumoniae and whole-cell degradation of oxynil herbicides and related compounds

Exogenous proline betaine (stachydrine or N-dimethylproline) or γ-butyrobetaine (γ-trimethylaminobutyrate), at a concentration as low as 1 mM, were found to stimulate the growth rate of Klebsiella pneumoniae, wild type M5A1, in media of inhibitory osmotic strength (0.8 M NaCl). Simultaneously, nitrogen fixation by whole cells, a process particularly sensitive to osmotic stress, was strongly enhanced by these compounds. However, in the absence of sodium chloride, both the growth and nitrogen fixation were not affected by the addition of the methylammonium derivatives in the medium. The sensitivity of the nitrogen fixation to osmotic stress was used as a bioassay to evaluate the potentiality of osmoprotective compound in relation to the number of methyl groups on the nitrogen atom of glycine, proline, and γ-aminobutyrate. Experiments with sarcosine (monomethylglycine), dimethylglycine, and glycine betaine (trimethylglycine), or experiments with mono- and di-methylproline or γ-mono-, γ-di-, γ-tri-methylaminobutyrate, indicated that the greatest stress tolerance was always obtained with the more N-methylated compounds.

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Phenotypic whole-cell screening identifies a protective carbohydrate epitope on Klebsiella pneumoniae

mAbs ◽

10.1080/19420862.2021.2006123 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Sophia K. Berry ◽

Steven Rust ◽

Carolina Caceres ◽

Lorraine Irving ◽

Josefin Bartholdson Scott ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Whole Cell ◽

Carbohydrate Epitope

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Functional Study of Lysine Decarboxylases from Klebsiella pneumoniae in Escherichia coli and Application of Whole Cell Bioconversion for Cadaverine Production

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1602.02030 ◽

2016 ◽

Vol 26 (9) ◽

pp. 1586-1592 ◽

Cited By ~ 6

Author(s):

Jung-Ho Kim ◽

Hyun Joong Kim ◽

Yong Hyun Kim ◽

Jong Min Jeon ◽

Hun Suk Song ◽

...

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Functional Study ◽

Whole Cell ◽

Whole Cell Bioconversion

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A high-throughput, whole cell assay to identify compounds active against carbapenem-resistant Klebsiella pneumoniae

PLoS ONE ◽

10.1371/journal.pone.0209389 ◽

2018 ◽

Vol 13 (12) ◽

pp. e0209389

Author(s):

Julie Liao ◽

George Xu ◽

Emily E. Mevers ◽

Jon Clardy ◽

Paula I. Watnick

Keyword(s):

Klebsiella Pneumoniae ◽

High Throughput ◽

Whole Cell ◽

Cell Assay ◽

Carbapenem Resistant

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Microbial Oxidation of Hydrocarbons and Related Compounds by Whole-Cell Suspensions of the Methane-Oxidizing Bacterium H-2

Applied and Environmental Microbiology ◽

10.1128/aem.52.6.1403-1406.1986 ◽

1986 ◽

Vol 52 (6) ◽

pp. 1403-1406 ◽

Cited By ~ 22

Author(s):

Takeo Imai ◽

Hirofumi Takigawa ◽

Satoshi Nakagawa ◽

Gwo-Jenn Shen ◽

Tohru Kodama ◽

...

Keyword(s):

Cell Suspensions ◽

Microbial Oxidation ◽

Whole Cell ◽

Oxidation Of Hydrocarbons ◽

Related Compounds

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HVEM Analysis of Microfilament Patterns in The Margins of Tissue Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600003512 ◽

1980 ◽

Vol 38 ◽

pp. 808-811

Author(s):

Carol Allen

Keyword(s):

Cell Movement ◽

Cell Spreading ◽

Living Cells ◽

Tissue Cell ◽

Large Sample Size ◽

Cell Periphery ◽

Whole Cell ◽

Critical Point Drying ◽

Lamellar Region ◽

Tissue Cells

When provided with a suitable solid substrate, tissue cells undergo a rapid conversion from the spherical form expressed in suspension culture to a characteristic flattened morphology. As a result of this conversion, called cell spreading, the cell nucleus and organelles come to occupy a central region of “deep cytoplasm” which slopes steeply into a peripheral “lamellar” region less than 1 pm thick at its outer edge and generally free of cell organelles. Cell spreading is accomplished by a continuous outward repositioning of the lamellar margins. Cell translocation on the substrate results when the activity of the lamellae on one side of the cell become dominant. When this occurs, the cell is “polarized” and moves in the direction of the “leading lamellae”. Careful analysis of tissue cell locomotion by time-lapse microphotography (1) has shown that the deformational movements of the leading lamellae occur in a repeating cycle of advance and retreat in the direction of cell movement and that the rate of such deformations are positively correlated with the speed of cell movement. In the present study, the physical basis for these movements of the cell margin has been examined by comparative light microscopy of living cells with whole-mount electron microscopy of fixed cells. Ultrastructural observations were made on tissue cells grown on Formvar-coated grids, fixed with glutaraldehyde, further processed by critical-point drying, and then photographed in the High Voltage Electron Microscope. This processing and imaging system maintains the 3-dimensional organization of the whole cell, the relationship of the cell to the substrate, and affords a large sample size which facilitates quantitative analysis. Comparative analysis of film records of living cells with the whole-cell micrographs revealed that specific patterns of microfilament organization consistently accompany recognizable stages of lamellar formation and movement. The margins of spreading cells and the leading lamellae of locomoting cells showed a similar pattern of MF repositionings (Figs. 1-4). These results will be discussed in terms of a working model for the mechanics of lamellar motility which includes the following major features: (a) lamellar protrusion results when an intracellular force is exerted at a locally weak area of the cell periphery; (b) the association of cortical MFs with one another determines the local resistance to this force; (c) where MF-to-MF association is weak, the cell periphery expands and some cortical MFs are dragged passively forward; (d) contact of the expanded area with the substrate then triggers the lateral association and reorientation of these cortical MFs into MF bundles parallel to the direction of the expansion; and (e) an active interaction between these MF bundles associated with the cortex of the expanded lamellae and the cortical MFs which remained in the sub-lamellar region then pulls the latter MFs forward toward the expanded area. Thus, the advance of the cell periphery on the substrate occurs in two stages: a passive phase in which some cortical MFs are dragged outward by the force acting to expand the cell periphery, and an active phase in which additional cortical MFs are pulled forward by interaction with the first set. Subsequent interactions between peripheral microfilament bundles and filaments in the deeper cytoplasm could then transmit the advance gained by lamellar expansion to the bulk of the cytoplasm.

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