Twenty-three substrains representing colonial variants of 11 strains of Actinabacillus mallei were examined for their ability to attack carbohydrates. Tests conducted in a basal liquid complex medium, containing yeast extract and proteose peptone No. 3 with bromcresol purple as indicator, showed that all strains tested produced acid from arabinose, glucose, fructose, galactose, mannose, and trehalose, while five substrains gave positive results with lactose, one with sucrose, and two with maltose. Eosin methylene blue agar of the same basal composition gave positive results for most of the strains grown on arabinose, glucose, fructose, galactose, mannose, and trehalose, and negative results for all strains grown on xylose, lactose, sucrose, and maltose. In a chemically defined medium containing ammonium chloride as nitrogen source and bromcresol purple as indicator, acid was produced by eight substrains of five of these strains from glucose, galactose, mannose, and trehalose, and by several strains from fructose and sucrose. The ability of these five selected strains to utilize carbohydrates as sole carbon sources for growth was tested in a chemically defined medium containing ammonium citrate as nitrogen source. All strains were able to grow on glucose, galactose, mannose, and trehalose, and most were able to grow on fructose. Arabinose, xylose, lactose, sucrose, and maltose did not support the growth of any of the strains tested.
Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry
