Recognition of naturally processed and ovarian cancer reactive CD8+ T cell epitopes within a promiscuous HLA class II T-helper region of NY-ESO-1

2008 ◽  
Vol 57 (8) ◽  
pp. 1185-1195 ◽  
Author(s):  
Junko Matsuzaki ◽  
Feng Qian ◽  
Immanuel Luescher ◽  
Shashikant Lele ◽  
Gerd Ritter ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
T Cell ◽  
Cd8 T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes ◽  
T Helper

P018. A long peptide from MELOE-1 contains multiple HLA class II T cell epitopes in addition to the HLA-A*0201 epitope

2011 ◽  
Vol 21 ◽  
pp. e26-e27
Author(s):  
A. Rogel ◽  
V. Vignard ◽  
M. Bobinet ◽  
N. Labarrière ◽  
F. Lang
Keyword(s):  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes

scholarly journals The identification of HLA class II-restricted T cell epitopes to vaccinia virus membrane proteins

Virology ◽  
2010 ◽  
Vol 408 (2) ◽  
pp. 232-240 ◽  
Author(s):  
Richard B. Kennedy ◽  
Gregory A. Poland
Keyword(s):  
T Cell ◽  
Membrane Proteins ◽  
Vaccinia Virus ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes

scholarly journals HLA-DR-Promiscuous T Cell Epitopes fromPlasmodiumfalciparumPre-Erythrocytic-Stage Antigens Restricted by Multiple HLA Class II Alleles

2000 ◽  
Vol 165 (2) ◽  
pp. 1123-1137 ◽  
Author(s):  
Denise L. Doolan ◽  
Scott Southwood ◽  
Robert Chesnut ◽  
Ettore Appella ◽  
Eduardo Gomez ◽  
...  
Keyword(s):  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes ◽  
Erythrocytic Stage ◽  
Hla Dr ◽  
Hla Class Ii Alleles

scholarly journals Scanning the HIV Genome for CD4+T Cell Epitopes Restricted to HLA-DP4, the Most Prevalent HLA Class II Molecule

2006 ◽  
Vol 176 (9) ◽  
pp. 5401-5408 ◽  
Author(s):  
William M. Cohen ◽  
Sandra Pouvelle-Moratille ◽  
Xiao-Fei Wang ◽  
Sandrine Farci ◽  
Gaetan Munier ◽  
...  
Keyword(s):  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
Cd4 T Cell ◽  
T Cell Epitopes

scholarly journals Development and validation of a broad scheme for prediction of HLA class II restricted T cell epitopes

2015 ◽  
Vol 422 ◽  
pp. 28-34 ◽  
Author(s):  
Sinu Paul ◽  
Cecilia S. Lindestam Arlehamn ◽  
Thomas J. Scriba ◽  
Myles B.C. Dillon ◽  
Carla Oseroff ◽  
...  
Keyword(s):  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes ◽  
Development And Validation

scholarly journals Identification of a Common HLA-DP4-Restricted T-Cell Epitope in the Conserved Region of the Respiratory Syncytial Virus G Protein

2004 ◽  
Vol 78 (4) ◽  
pp. 1775-1781 ◽  
Author(s):  
L. de Waal ◽  
S. Yüksel ◽  
A. H. Brandenburg ◽  
J. P. M. Langedijk ◽  
K. Sintnicolaas ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
T Cell ◽  
Matrix Protein ◽  
Mononuclear Cells ◽  
Cell Epitope ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes ◽  
Syncytial Virus ◽  
Tract Infections

ABSTRACT The cellular immune response to respiratory syncytial virus (RSV) is important in both protection and immunopathogenesis. In contrast to HLA class I, HLA class II-restricted RSV-specific T-cell epitopes have not been identified. Here, we describe the generation and characterization of two human RSV-specific CD4+-T-cell clones (TCCs) associated with type 0-like cytokine profiles. TCC 1 was specific for the matrix protein and restricted over HLA-DPB1*1601, while TCC 2 was specific for the attachment protein G and restricted over either HLA-DPB1*0401 or -0402. Interestingly, the latter epitope is conserved in both RSV type A and B viruses. Given the high allele frequencies of HLA-DPB1*0401 and -0402 worldwide, this epitope could be widely recognized and boosted by recurrent RSV infections. Indeed, peptide stimulation of peripheral blood mononuclear cells from healthy adults resulted in the detection of specific responses in 8 of 13 donors. Additional G-specific TCCs were generated from three of these cultures, which recognized the identical (n = 2) or almost identical (n = 1) HLA-DP4-restricted epitope as TCC 2. No significant differences were found between the capacities of cell lines obtained from infants with severe (n = 41) or mild (n = 46) RSV lower respiratory tract infections to function as antigen-presenting cells to the G-specific TCCs, suggesting that the severity of RSV disease is not linked to the allelic frequency of HLA-DP4. In conclusion, we have identified an RSV G-specific human T helper cell epitope restricted by the widely expressed HLA class II alleles DPB1*0401 and -0402. Its putative role in protection and/or immunopathogenesis remains to be determined.

scholarly journals MELOE-1 Antigen Contains Multiple HLA Class II T Cell Epitopes Recognized by Th1 CD4+ T Cells from Melanoma Patients

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51716 ◽  
Author(s):  
Mathilde Bobinet ◽  
Virginie Vignard ◽  
Anne Rogel ◽  
Amir Khammari ◽  
Brigitte Dreno ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd4 T Cells ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Epitopes ◽  
Melanoma Patients

scholarly journals Minimal T-Cell-Stimulatory Sequences and Spectrum of HLA Restriction of Immunodominant CD4+ T-Cell Epitopes within Hepatitis C Virus NS3 and NS4 Proteins

2005 ◽  
Vol 79 (19) ◽  
pp. 12425-12433 ◽  
Author(s):  
J. T. Gerlach ◽  
A. Ulsenheimer ◽  
N. H. Grüner ◽  
M.-C. Jung ◽  
W. Schraut ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Acute Hepatitis ◽  
Patient Selection ◽  
Class Ii ◽  
Hla Class Ii ◽  
Acute Hepatitis C ◽  
T Cell Epitopes

ABSTRACT The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed characterization of immunodominant CD4+ T-cell epitopes is required. We studied peripheral blood mononuclear cells from 20 patients with acute hepatitis C using 83 overlapping 20-mer peptides covering the NS3 helicase and NS4. Eight peptides were recognized by ≥40% of patients, and specific CD4+ T-cell clones were obtained for seven of these and three additional, subdominant epitopes. Mapping of minimal stimulatory sequences defined epitopes of 8 to 13 amino acids in length, but optimal T-cell stimulation was observed with 10- to 15-mers. While some epitopes were presented by different HLA molecules, others were presented by only a single HLA class II molecule, which has implications for patient selection in clinical trials of peptide-based immunotherapies. In conclusion, using two different approaches we identified and characterized a set of CD4+ T-cell epitopes in the HCV NS3-NS4 region which are immunodominant in patients achieving transient or persistent viral control. This information allows the construction of a valuable panel of HCV-specific HLA class II tetramers for further study of CD4+ T-cell responses in chronic hepatitis C. The finding of immunodominant epitopes with very constrained HLA restriction has implications for patient selection in clinical trials of peptide-based immunotherapies.

Vaccine-Induced, Pseudorabies Virus-Specific, Extrathymic CD4+CD8+ Memory T-Helper Cells in Swine

1998 ◽  
Vol 72 (6) ◽  
pp. 4866-4873 ◽  
Author(s):  
Bertram T. Ober ◽  
Artur Summerfield ◽  
Christina Mattlinger ◽  
Karl-Heinz Wiesmüller ◽  
Günther Jung ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Mhc Class Ii ◽  
T Helper Cells ◽  
Pseudorabies Virus ◽  
Class Ii ◽  
T Cell Epitopes ◽  
T Helper ◽  
Helper Cells

ABSTRACT Pseudorabies virus (PRV; suid herpesvirus 1) infection causes heavy economic losses in the pig industry. Therefore, vaccination with live attenuated viruses is practiced in many countries. This vaccination was demonstrated to induce extrathymic virus-specific memory CD4+CD8+ T lymphocytes. Due to their major histocompatibility complex (MHC) class II-restricted proliferation, it is generally believed that these T lymphocytes function as memory T-helper cells. To directly prove this hypothesis, 15-amino-acid, overlapping peptides of the viral glycoprotein gC were used for screening in proliferation assays with peripheral blood mononuclear cells of vaccinated d/d haplotype inbred pigs. In these experiments, two naturally processed T-cell epitopes (T1 and T2) which are MHC class II restricted were identified. It was shown that extrathymic CD4+CD8+ T cells are the T-lymphocyte subpopulation that responds to epitope T2. In addition, we were able to show that cytokine secretion can be induced in these T cells through recall with inactivated PRV and demonstrated that activated PRV-primed CD4+CD8+ T cells are able to induce PRV-specific immunoglobulin synthesis by PRV-primed, resting B cells. Taken together, these results demonstrate that the glycoprotein gC takes part in the priming of humoral anti-PRV memory responses. The experiments identified the first T-cell epitopes so far known to induce the generation of virus-specific CD4+CD8+ memory T lymphocytes and showed that CD4+CD8+ T cells are memory T-helper cells. Therefore, this study describes the generation of virus-specific CD4+CD8+ T cells, which is observed during vaccination, as a part of the potent humoral anti-PRV memory response induced by the vaccine.

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