Detection of SET-NUP214 rearrangement using multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) in acute leukemias: a case report and literature review on a Korean case series

2011 ◽  
Vol 91 (7) ◽  
pp. 1135-1138 ◽  
Author(s):  
Eun Young Lee ◽  
Tae Sung Park ◽  
Min Jin Kim ◽  
Myung Hee Chang ◽  
Eun Hae Cho ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Case Report ◽  
Literature Review ◽  
Reverse Transcriptase ◽  
Case Series ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Acute Leukemias ◽  
Polymerase Chain

scholarly journals Case Report: Acute Intracardiac Thrombosis in Children With Coronavirus Disease 2019 (COVID-19)

2021 ◽  
Vol 9 ◽  
Author(s):  
Hamid Bigdelian ◽  
Mohsen Sedighi ◽  
Mohammad Reza Sabri ◽  
Bahar Dehghan ◽  
Chehreh Mahdavi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cardiac Surgery ◽  
Case Report ◽  
Case Series ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Inflammatory State ◽  
Polymerase Chain ◽  
Underlying Mechanisms ◽  
Intracardiac Thrombosis

We herein describe a case series of children with SARS-CoV-2 infection (COVID-19) complicated with acute intracardiac thrombosis. The diagnosis of COVID-19 was confirmed through the reverse transcription-polymerase chain reaction (RT-PCR). Transthoracic echocardiography of patients revealed large intracardiac mobile masses resected successfully via cardiac surgery. The underlying mechanisms of this thrombus in the COVID-19 infection may be attributed to the hypercoagulation and inflammatory state of the disease incurred by the SARS-CoV-2 virus.

scholarly journals Multiple Negative of RT-PCR Testing of COVID-19 Pneumonia: A Case Report

2020 ◽  
Author(s):  
HuiLi ◽  
Junton Feng ◽  
Lamei Chen ◽  
Yinhao Wu ◽  
Yan Su ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Case Report ◽  
Reverse Transcriptase ◽  
Clinical Manifestation ◽  
Lymphocyte Count ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Case Presentation ◽  
Polymerase Chain ◽  
Novel Coronavirus

Abstract Background: Since the 2019 novel coronavirus outbreak in Wuhan, Hubei Province, China in 2019, there have been a few reports of multiple negative of RT-PCR tests in patients infected by 2019 novel coronavirus.Case presentation: The patient was a 64-year-old man with fever. His son returned from Hubei 17 days before the patient had fever. Ancillary examinations indicated a decreased lymphocyte count and ground-glass opacities in lung. However, the first five reverse transcriptase polymerase chain reaction tests of 2019 novel coronavirus were negative until the sixth turned to positive.Conclusion: when epidemiological history, clinical manifestation and imaging are highly suggestive of COVID-19 Pneumonia, we should repeat reverse transcriptase polymerase chain reaction tests and treat patients in isolation.

Culture and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Proven Mycobacterium Tuberculosis Endophthalmitis: A Case Series

2016 ◽  
Vol 26 (2) ◽  
pp. 220-227 ◽  
Author(s):  
Ekta Rishi ◽  
Pukhraj Rishi ◽  
K. Lily Therese ◽  
Gayathri Ramasubban ◽  
Jyotirmay Biswas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Reverse Transcriptase ◽  
Case Series ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Chilblains-Like Lesions as a Presenting Manifestation of COVID-19

2020 ◽  
Vol 4 (5) ◽  
pp. 440-442
Author(s):  
Victoria Comeau ◽  
Marcus Goodman ◽  
Carmen Julian ◽  
Francesca M Ceci
Keyword(s):  
Polymerase Chain Reaction ◽  
Case Report ◽  
Reverse Transcriptase ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Cutaneous Lesions ◽  
Polymerase Chain ◽  
Systemic Symptoms ◽  
Dermatologic Manifestations

Dermatologic manifestations associated with coronavirus disease 2019 (COVID-19) remain broad and nonspecific. While a surge of “COVID toes” have been reported worldwide, very few cases have been documented in reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed COVID-19 patients. Furthermore, majority of confirmed COVID-19 patients with chilblains-like lesions presented with cutaneous lesions localized to the toes, which was preceded by other systemic symptoms. Our case report describes an occurrence of chilblains-like lesions, limited to the fingers, in an asymptomatic COVID-19 patient confirmed by RT-PCR. 

scholarly journals A meta-analysis of accuracy and sensitivity of chest CT and RT-PCR in COVID-19 diagnosis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fatemeh Khatami ◽  
Mohammad Saatchi ◽  
Seyed Saeed Tamehri Zadeh ◽  
Zahra Sadat Aghamir ◽  
Alireza Namazi Shabestari ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Ct Scan ◽  
Predictive Value ◽  
Meta Analysis ◽  
Chest Ct ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Chest Ct Scan

AbstractNowadays there is an ongoing acute respiratory outbreak caused by the novel highly contagious coronavirus (COVID-19). The diagnostic protocol is based on quantitative reverse-transcription polymerase chain reaction (RT-PCR) and chests CT scan, with uncertain accuracy. This meta-analysis study determines the diagnostic value of an initial chest CT scan in patients with COVID-19 infection in comparison with RT-PCR. Three main databases; PubMed (MEDLINE), Scopus, and EMBASE were systematically searched for all published literature from January 1st, 2019, to the 21st May 2020 with the keywords "COVID19 virus", "2019 novel coronavirus", "Wuhan coronavirus", "2019-nCoV", "X-Ray Computed Tomography", "Polymerase Chain Reaction", "Reverse Transcriptase PCR", and "PCR Reverse Transcriptase". All relevant case-series, cross-sectional, and cohort studies were selected. Data extraction and analysis were performed using STATA v.14.0SE (College Station, TX, USA) and RevMan 5. Among 1022 articles, 60 studies were eligible for totalizing 5744 patients. The overall sensitivity, specificity, positive predictive value, and negative predictive value of chest CT scan compared to RT-PCR were 87% (95% CI 85–90%), 46% (95% CI 29–63%), 69% (95% CI 56–72%), and 89% (95% CI 82–96%), respectively. It is important to rely on the repeated RT-PCR three times to give 99% accuracy, especially in negative samples. Regarding the overall diagnostic sensitivity of 87% for chest CT, the RT-PCR testing is essential and should be repeated to escape misdiagnosis.

scholarly journals Use of immunodot blot and multiplex reverse transcriptase-polymerase chain reaction in dengue virus detection in macerates of Aedes aegypti larvae

2012 ◽  
Vol 3 (1) ◽  
pp. 13
Author(s):  
Aline T.A. Chagas ◽  
Michelle D. Oliveira ◽  
Jose M.S. Mezencio ◽  
Eduardo A.M. Silva ◽  
Leandro L. Oliveira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Epidemiological Surveillance ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Denv Serotypes ◽  
Polymerase Chain ◽  
Pcr Techniques

The <em>Dengue virus</em> is the main arbovirus that affects man in terms of morbidity and mortality. The detection of the virus is very important for epidemiological surveillance, so here we propose to standardize and compare the immunodot blot (IDB) and multiplex reverse transcriptase-polymerase chain reaction (M-RT-PCR) techniques to detect and characterize the dengue virus (DENV) serotypes in samples of <em>Aedes aegypti</em> larvae. Thus, the IDB and M-RT-PCR techniques were standardized using macerated samples of larvae collected in nature. The use of monoclonal antibodies in IDB has not shown great results, but DENV detection through this method was possible using polyclonal antibodies. The distinction of serotypes 1, 2 and 3 was carried out by M-RT-PCR.

scholarly journals Deteksi Molekuler Virus Dengue dan Chikungunya pada Nyamuk Aedes spp. di Kecamatan Cilongok

2020 ◽  
Vol 2 (2) ◽  
pp. 181
Author(s):  
Dwi Iva Fitriana ◽  
Endang Srimurni Kusmintarsih ◽  
Trisnowati Budi Ambarningrum
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

DBD dan chikungunya merupakan salah satu penyakit yang masih menjadi masalah di Indonesia. Kecamatan Cilongok merupakan salah kecamatan endemis DBD dan pernah mengalami KLB chikungunya. Deteksi virus pada nyamuk sebelum menginfeksi manusia penting sebagai peringatan dini dalam upaya pencegahan wabah di daerah endemis. Tujuan penelitian ini adalah mengetahui infeksi virus Dengue dan Chikungunya pada nyamuk Aedes spp. yang ditangkap. Penelitian ini dilakukan di empat desa di Kecamatan Cilongok yang meliputi Desa Cilongok, Pernasidi, Kalisari, dan Jatisaba, pengambilan sampel dilakukan secara purposive. Deteksi virus Dengue dan Chikungunya pada nyamuk dilakukan menggunakan metode Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR). Hasil positifitas virus dianalisis secara deskriptif untuk menggambarkan potensi transmisi virus. Hasil penelitian menunjukkan bahwa nyamuk Aedes spp. yang tertangkap tidak mengandung virus Dengue dan Chikungunya.  

scholarly journals A reverse transcriptase-polymerase chain reaction detects heterogeneous chimeric mRNAs in leukemias with 11q23 abnormalities

Blood ◽  
1994 ◽  
Vol 83 (10) ◽  
pp. 2912-2921 ◽  
Author(s):  
K Yamamoto ◽  
M Seto ◽  
S Iida ◽  
H Komatsu ◽  
N Kamada ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Response To Therapy ◽  
Chromosome 9 ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Single Clone ◽  
Sensitivity Studies ◽  
Polymerase Chain ◽  
11Q23 Abnormalities

Abstract The MLL gene involved in 11q23 translocations found in the majority of infantile leukemias and some secondary leukemias makes fusion transcripts with genes such as LTG4 (chromosome 4), LTG9 (chromosome 9), and LTG19 (chromosome 19) as a result of reciprocal translocation. We have examined 25 cases of leukemias with 11q23 abnormalities by Southern blot analysis and the reverse transcriptase-polymerase chain reaction (RT-PCR). Using various primer pairs, chimeric mRNAs could be amplified in 6 of 7 leukemias with t(4;11), 6 of 8 leukemias with t(9;11) including secondary leukemia, 8 of 9 leukemias with t(11;19), and 1 with a deletion at 11q23. The chimeric mRNAs were heterogeneous and differential usage of the MLL exons was found, irrespective of the partner chromosomes. Sensitivity studies showed that a single clone with chimeric mRNA in 10(4) to 10(5) cells could be detected. These findings show that the present RT-PCR settings provide a rapid, accurate, and sensitive tool for diagnosing leukemias with 11q23 translocations and for monitoring response to therapy in these patients.

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