Clinical significance of circulating tumor cells in the response to trastuzumab for HER2-negative metastatic gastric cancer

2021 ◽  
Author(s):  
Daisuke Matsushita ◽  
Yoshikazu Uenosono ◽  
Takaaki Arigami ◽  
Shigehiro Yanagita ◽  
Keishi Okubo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Significance ◽  
Metastatic Gastric Cancer

scholarly journals Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer

Oncotarget ◽  
2014 ◽  
Vol 5 (16) ◽  
pp. 6594-6602 ◽  
Author(s):  
Yilin Li ◽  
Xiaotian Zhang ◽  
Sai Ge ◽  
Jing Gao ◽  
Jifang Gong ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Advanced Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Significance

scholarly journals Wedge-shaped microfluidic chip for circulating tumor cells isolation and its clinical significance in gastric cancer

2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Chaogang Yang ◽  
Nangang Zhang ◽  
Shuyi Wang ◽  
Dongdong Shi ◽  
Chunxiao Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Significance ◽  
Microfluidic Chip

scholarly journals Clinical significance of circulating tumor cells in blood from patients with gastric cancer

2017 ◽  
Vol 1 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Takaaki Arigami ◽  
Yoshikazu Uenosono ◽  
Shigehiro Yanagita ◽  
Keishi Okubo ◽  
Takashi Kijima ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Significance

scholarly journals Clinical significance of circulating tumor cells in gastric cancer patients

Oncotarget ◽  
2017 ◽  
Vol 8 (15) ◽  
pp. 25713-25720 ◽  
Author(s):  
Jitao Zhou ◽  
Xin Ma ◽  
Feng Bi ◽  
Ming Liu
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Significance ◽  
Gastric Cancer Patients

scholarly journals Transcriptomic profiling of single circulating tumor cells provides insight into human metastatic gastric cancer

2022 ◽  
Vol 5 (1) ◽  
Author(s):  
Ryo Negishi ◽  
Hitomi Yamakawa ◽  
Takeru Kobayashi ◽  
Mayuko Horikawa ◽  
Tatsu Shimoyama ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Transcriptome Analysis ◽  
Epithelial Mesenchymal Transition ◽  
Metastatic Gastric Cancer ◽  
Isolation Technique ◽  
Mesenchymal Transition ◽  
Single Ctcs

AbstractTranscriptome analysis of circulating tumor cells (CTCs), which migrate into blood vessels from primary tumor tissues, at the single-cell level offers critical insights into the biology of metastasis and contributes to drug discovery. However, transcriptome analysis of single CTCs has only been reported for a limited number of cancer types, such as multiple myeloma, breast, hepatocellular, and prostate cancer. Herein, we report the transcriptome analysis of gastric cancer single-CTCs. We utilized an antigen-independent strategy for CTC isolation from metastatic gastric cancer patients involving a size-dependent recovery of CTCs and a single cell isolation technique. The transcriptomic profile of single-CTCs revealed that a majority of gastric CTCs had undergone epithelial-mesenchymal transition (EMT), and indicated the contribution of platelet adhesion toward EMT progression and acquisition of chemoresistance. Taken together, this study serves to employ CTC characterization to elucidate the mechanisms of chemoresistance and metastasis in gastric cancer.

scholarly journals Clinical significance of circulating tumor cells in peripheral blood from patients with gastric cancer

Cancer ◽  
2013 ◽  
Vol 119 (22) ◽  
pp. 3984-3991 ◽  
Author(s):  
Yoshikazu Uenosono ◽  
Takaaki Arigami ◽  
Tsutomu Kozono ◽  
Shigehiro Yanagita ◽  
Takahiko Hagihara ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Significance ◽  
Peripheral Blood

scholarly journals Circulating Tumor Cells and TWIST Expression in Patients with Metastatic Gastric Cancer: A Preliminary Study

2021 ◽  
Vol 10 (19) ◽  
pp. 4481
Author(s):  
Joon Hyung Jhi ◽  
Gwang Ha Kim ◽  
Su Jin Park ◽  
Dong Uk Kim ◽  
Moon Won Lee ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Cancer ◽  
Metastatic Gastric Cancer ◽  
Microfluidic System ◽  
Hematogenous Metastasis ◽  
Twist Expression ◽  
Preliminary Study ◽  
Chemotherapy Patients

Background and Aims: The clinical significance of circulating tumor cells (CTCs) and TWIST expression in CTCs remains unelucidated in patients with gastric cancer (GC). Here, we evaluated CTCs and TWIST expression in CTCs and explored their correlation with prognosis in patients with metastatic GC. Methods: Peripheral blood samples were prospectively obtained from 31 patients with metastatic GC between September 2017 and December 2018, prior to treatment. CTCs were detected using a centrifugal microfluidic system and CTCs positive for TWIST immunostaining were defined as TWIST (+) CTCs. Results: CTCs and TWIST (+) CTCs were detected in 25 (80.6%) and 24 (77.4%) of the 31 patients, respectively. CTC count in patients with first diagnosis of metastatic cancer tended to be higher than that in those with recurrent metastatic cancer, but TWIST (+) CTC count was not different between the two groups. There was no difference in CTC and TWIST (+) CTC counts according to histopathologic type, peritoneal dissemination, hematogenous metastasis, serum tumor makers, or response to first-line chemotherapy. Patients with CTCs > 7.5/7.5 mL of blood showed shorter overall survival (OS) than those with CTCs ≤ 7.5/7.5 mL of blood (p = 0.049). Additionally, patients with TWIST (+) CTCs > 2.5/7.5 mL of blood tended to show shorter OS than those with TWIST (+) CTCs ≤ 2.5/7.5 mL of blood (p = 0.105). Conclusions: Our study demonstrated that high levels of CTCs and TWIST (+) CTCs were associated with worse OS.

scholarly journals Circulating Tumor Cells and TWIST Expression in Patients with Metastatic Gastric Cancer

2020 ◽  
Author(s):  
Joon Hyung Jhi ◽  
Gwang Ha Kim ◽  
Su Jin Park ◽  
Dong Uk Kim ◽  
Moon Won Lee ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Cancer ◽  
Metastatic Gastric Cancer ◽  
Microfluidic System ◽  
Hematogenous Metastasis ◽  
First Line ◽  
Twist Expression ◽  
Chemotherapy Patients

Abstract Background: The clinical significance of circulating tumor cells (CTCs) and TWIST expression in CTCs remains unelucidated in patients with gastric cancer (GC). Here, we evaluated CTCs and TWIST expression in CTCs and explored their correlation with prognosis in patients with metastatic GC. Methods: Peripheral blood samples were prospectively obtained from 31 patients with metastatic GC between September 2017 and December 2018, prior to treatment. CTCs were detected using a centrifugal microfluidic system and CTCs positive for TWIST immunostaining were defined as TWIST (+) CTCs. CTCs and TWIST (+) Results: CTCs were detected in 25 (80.6%) of the 31 patients. CTC count in patients with first diagnosis of metastatic cancer tended to be higher than that in those with recurrent metastatic cancer, but TWIST (+) CTC count was not different between the two groups. There was no difference in CTC and TWIST (+) CTC counts according to histopathologic type, peritoneal dissemination, hematogenous metastasis, serum tumor makers, or response to first-line chemotherapy. Patients with CTCs >7.5/7.5 mL of blood showed shorter overall survival (OS) than those with CTCs ≤7.5/7.5 mL of blood (P = 0.049). Additionally, patients with TWIST (+) CTCs >2.5/7.5 mL of blood showed shorter OS than those with TWIST (+) CTCs ≤2.5/7.5 mL of blood (P = 0.105). Conclusions: Our study demonstrated that high levels of CTCs and TWIST (+) CTCs were associated with worse OS.

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