scholarly journals Analysis of intrapatient heterogeneity of circulating tumor cells at the single-cell level in the cerebrospinal fluid of a patient with metastatic gastric cancer

2020 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
SeungTae Kim ◽  
JangHo Cho ◽  
Moon-Hee Sim ◽  
SunYoung Kim ◽  
Kyung Kim ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cerebrospinal Fluid ◽  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Gastric Cancer ◽  
Single Cell Level ◽  
Cell Level

scholarly journals Transcriptomic profiling of single circulating tumor cells provides insight into human metastatic gastric cancer

2022 ◽  
Vol 5 (1) ◽  
Author(s):  
Ryo Negishi ◽  
Hitomi Yamakawa ◽  
Takeru Kobayashi ◽  
Mayuko Horikawa ◽  
Tatsu Shimoyama ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Transcriptome Analysis ◽  
Epithelial Mesenchymal Transition ◽  
Metastatic Gastric Cancer ◽  
Isolation Technique ◽  
Mesenchymal Transition ◽  
Single Ctcs

AbstractTranscriptome analysis of circulating tumor cells (CTCs), which migrate into blood vessels from primary tumor tissues, at the single-cell level offers critical insights into the biology of metastasis and contributes to drug discovery. However, transcriptome analysis of single CTCs has only been reported for a limited number of cancer types, such as multiple myeloma, breast, hepatocellular, and prostate cancer. Herein, we report the transcriptome analysis of gastric cancer single-CTCs. We utilized an antigen-independent strategy for CTC isolation from metastatic gastric cancer patients involving a size-dependent recovery of CTCs and a single cell isolation technique. The transcriptomic profile of single-CTCs revealed that a majority of gastric CTCs had undergone epithelial-mesenchymal transition (EMT), and indicated the contribution of platelet adhesion toward EMT progression and acquisition of chemoresistance. Taken together, this study serves to employ CTC characterization to elucidate the mechanisms of chemoresistance and metastasis in gastric cancer.

scholarly journals Genomic Analysis of Circulating Tumor Cells at the Single-Cell Level

2020 ◽  
Vol 22 (6) ◽  
pp. 770-781 ◽  
Author(s):  
Shan Lu ◽  
Chia-Jung Chang ◽  
Yinghui Guan ◽  
Edith Szafer-Glusman ◽  
Elizabeth Punnoose ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Genomic Analysis ◽  
Single Cell Level ◽  
Cell Level

A microfluidic platform for high-purity separating circulating tumor cells at the single-cell level

Talanta ◽  
2019 ◽  
Vol 200 ◽  
pp. 169-176 ◽  
Author(s):  
Kun Wang ◽  
Lin Zhou ◽  
Simin Zhao ◽  
Zule Cheng ◽  
Shihui Qiu ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Purity ◽  
Single Cell Level ◽  
Cell Level ◽  
Microfluidic Platform

scholarly journals Heterogeneity of PIK3CA mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients

2014 ◽  
Vol 9 (4) ◽  
pp. 749-757 ◽  
Author(s):  
Marta Pestrin ◽  
Francesca Salvianti ◽  
Francesca Galardi ◽  
Francesca De Luca ◽  
Natalie Turner ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Single Cell ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Metastatic Breast ◽  
Single Cell Level ◽  
Breast Cancer Patients ◽  
Cell Level ◽  
Mutational Status

scholarly journals Combined Molecular Genetic and Cytogenetic Analysis from Single Cells after Isothermal Whole-Genome Amplification

2011 ◽  
Vol 57 (7) ◽  
pp. 1032-1041 ◽  
Author(s):  
Thomas Kroneis ◽  
Jochen B Geigl ◽  
Amin El-Heliebi ◽  
Martina Auer ◽  
Peter Ulz ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Whole Genome Amplification ◽  
Single Cells ◽  
Molecular Genetic ◽  
Target Cells ◽  
Whole Genome ◽  
Single Cell Level ◽  
Cell Level ◽  
Genome Amplification

BACKGROUND Analysis of chromosomal aberrations or single-gene disorders from rare fetal cells circulating in the blood of pregnant women requires verification of the cells' genomic identity. We have developed a method enabling multiple analyses at the single-cell level that combines verification of the genomic identity of microchimeric cells with molecular genetic and cytogenetic diagnosis. METHODS We used a model system of peripheral blood mononuclear cells spiked with a colon adenocarcinoma cell line and immunofluorescence staining for cytokeratin in combination with DNA staining with the nuclear dye TO-PRO-3 in a preliminary study to define candidate microchimeric (tumor) cells in Cytospin preparations. After laser microdissection, we performed low-volume on-chip isothermal whole-genome amplification (iWGA) of single and pooled cells. RESULTS DNA fingerprint analysis of iWGA aliquots permitted successful identification of all analyzed candidate microchimeric cell preparations (6 samples of pooled cells, 7 samples of single cells). Sequencing of 3 single-nucleotide polymorphisms was successful at the single-cell level for 20 of 32 allelic loci. Metaphase comparative genomic hybridization (mCGH) with iWGA products of single cells showed the gains and losses known to be present in the genomic DNA of the target cells. CONCLUSIONS This method may be instrumental in cell-based noninvasive prenatal diagnosis. Furthermore, the possibility to perform mCGH with amplified DNA from single cells offers a perspective for the analysis of nonmicrochimeric rare cells exhibiting genomic alterations, such as circulating tumor cells.

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