Effects of gemcitabine and araC on in vitro DNA synthesis mediated by the human breast cell DNA synthesome

2000 ◽  
Vol 45 (4) ◽  
pp. 320-328 ◽  
Author(s):  
Hai Yan Jiang ◽  
Robert J. Hickey ◽  
Waleed Abdel-Aziz ◽  
Linda H. Malkas
Keyword(s):  
Dna Synthesis ◽  
Human Breast ◽  
Dna Synthesome ◽  
Human Breast Cell

Toremifene: pharmacologic and pharmacokinetic basis of reversing multidrug resistance.

1989 ◽  
Vol 7 (9) ◽  
pp. 1359-1364 ◽  
Author(s):  
M W DeGregorio ◽  
J M Ford ◽  
C C Benz ◽  
V J Wiebe
Keyword(s):  
Estrogen Receptor Status ◽  
Plasma Concentrations ◽  
Multidrug Resistant ◽  
High Dose ◽  
Human Breast ◽  
Human Breast Cell Line ◽  
Human Breast Cell ◽  
Mcf 7 ◽  
Resistant Cells

Triphenylethylene compounds, such as tamoxifen, have shown chemosensitizing activity independent of estrogen receptor status in doxorubicin-resistant cells. We examined the chemosensitizing activity of a new triphenylethylene, toremifene, and its major metabolites in a doxorubicin-resistant human breast cell line, MCF-7/DOX. In addition, we examined the chemosensitizing activity of unbound plasma toremifene and its metabolites isolated from patients treated with toremifene doses of 20 to 400 mg/d. MCF-7/DOX cells were exposed to ultrafiltrate plasma specimens in the absence and presence of doxorubicin. These latter studies were single-blinded. Toremifene and its major metabolites were capable of sensitizing multidrug-resistant cells to doxorubicin. The degree of chemosensitizing activity in vitro correlated with the plasma concentrations of toremifene and its metabolites (P less than .05). Plasma samples isolated from patients receiving high-dose toremifene (400 mg/d) had the greatest chemosensitizing activity. We present evidence that toremifene and its metabolites can sensitize resistant MCF-7/DOX cells to doxorubicin, that this effect is concentration-dependent, and that sensitizing activity can be detected at clinically achieved concentrations.

Melatonin inhibits dna synthesis in mcf-7 human breast cancer cells in vitro

Life Sciences ◽  
1996 ◽  
Vol 58 (26) ◽  
pp. 2447-2453 ◽  
Author(s):  
S. Cos ◽  
F. Fernández ◽  
E.J. Sánchez-Barceló
Keyword(s):  
Breast Cancer ◽  
Dna Synthesis ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

BREAST CARCINOMAS SYNTHESIZE FACTORS WHICH INFLUENCE OSTEOBLAST-LIKE CELLS INDEPENDENTLY OF OSTEOCLASTS IN VITRO

1991 ◽  
Vol 128 (3) ◽  
pp. R5-R8 ◽  
Author(s):  
C E Evans ◽  
C Ward ◽  
I P Braidman
Keyword(s):  
Breast Cancer ◽  
Dna Synthesis ◽  
Mammary Carcinoma ◽  
Carcinoma Cells ◽  
Collagen Gels ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Human Breast Carcinomas ◽  
Mammary Carcinoma Cells

ABSTRACT Bone metastases in breast cancer may be osteolytic, osteosclerotic, or a mixture of the two. Although stimulation of bone resorption by breast cancer cells has attracted some interest, the formation of osteosclerotic secondary tumours and the influence of human mammary carcinoma cells on osteoblasts (bone forming cells), both important in understanding breast cancer - bone interactions, have been largely neglected. We therefore examined the effects of conditioned medium (CM) from two cultured human breast cancer cell lines (MCF7 and ZR-75) and from primary cultures of breast carcinomas from two patients, on osteoblasts and recruitment of bone-resorbing cells (osteoclasts) in vitro. Osteoblast-like cells (BDC) were cultured from human trabecular bone explants. Osteoclast maturation was studied in fetal rat calvaria cultured on collagen gels. CM from the MCF-7 line and cells derived from one patient each inhibited BDC DNA synthesis, but stimulated osteoclast recruitment. In contrast, CM from the second patient's cells or ZR-75 enhanced DNA synthesis in BDC, but blocked osteoclast maturation. This suggests that human breast carcinomas secrete soluble factors which influence both osteoclasts and osteoblasts. A further unexpected implication is that mammary carcinoma cells may cause local osteosclerosis by directly stimulating osteoblasts, rather than through raised bone turnover in metastases.

scholarly journals Soya phytonutrients act on a panel of genes implicated with BRCA1and BRCA2 oncosuppressors in human breast cell lines

2006 ◽  
Vol 95 (2) ◽  
pp. 406-413 ◽  
Author(s):  
Bertrand Caëtano ◽  
Ludovic Le Corre ◽  
Nassera Chalabi ◽  
Laetitia Delort ◽  
Yves-Jean Bignon ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Response To Treatment ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Ki 67 ◽  
Breast Cell Lines ◽  
Human Breast Cell ◽  
Mcf 7

Breast cancer is the most common cancer in women and a significant cause of death. Mutations of the oncosuppressor genes BRCA1 and BRCA2 are associated with a hereditary risk of breast cancer, and dysregulation of their expression has been observed in sporadic cases. Soya isoflavones have been shown to inhibit breast cancer in studies in vitro, but associations between the consumption of isoflavone-containing foods and breast cancer risk have varied in epidemiological studies. Soya is a unique source of the phytoestrogens daidzein (4′,7-dihydroxyisoflavone) and genistein (4′,5,7-trihydroxyisoflavone), two molecules that are able to inhibit the proliferation of human breast cancer cells in vitro. The aim of the present study was to determine the effects of genistein (5μg/ml) and daidzein (20μg/ml) on transcription in three human breast cell lines (one dystrophic, MCF10a, and two malignant, MCF-7 and MDA-MB-231) after 72h treatment. The different genes involved in the BRCA1 and BRCA2 pathways (GADD45A, BARD1, JUN, BAX, RB1, ERα, ERβ, BAP1, TNFα, p53, p21Waf1/Cip1, p300, RAD51, pS2, Ki-67) were quantified by real-time quantitative RT-PCR, using the TaqMan method and an ABI Prism 7700 Sequence Detector (Applied Biosystems). We observed that, in response to treatment, many of these genes were overexpressed in the breast cancer cell lines (MCF-7 and MDA-MB-231) but not in the dystrophic cell line (MCF10a).

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