The Aspergillus nidulans amdS gene as a marker for the identification of multicopy T-DNA integration events in Agrobacterium-mediated transformation of Aspergillus awamori

2004 ◽  
Vol 45 (6) ◽  
pp. 399-403 ◽  
Author(s):  
C. B. Michielse ◽  
A. F. J. Ram ◽  
C. A. M. J. J. van den Hondel
Keyword(s):  
Aspergillus Nidulans ◽  
Aspergillus Awamori ◽  
Dna Integration ◽  
Agrobacterium Mediated Transformation ◽  
Amds Gene

scholarly journals Agrobacterium-Mediated Transformation of Aspergillus awamori in the Absence of Full-Length VirD2, VirC2, or VirE2 Leads to Insertion of Aberrant T-DNA Structures

2004 ◽  
Vol 186 (7) ◽  
pp. 2038-2045 ◽  
Author(s):  
Caroline B. Michielse ◽  
Arthur F. J. Ram ◽  
Paul J. J. Hooykaas ◽  
Cees A. M. J. J. van den Hondel
Keyword(s):  
Single Copy ◽  
Full Length ◽  
Aspergillus Awamori ◽  
Wild Type ◽  
Dna Structures ◽  
Dna Integration ◽  
Virulence Proteins ◽  
Type Transformation ◽  
The Right ◽  
Transposon Insertions

ABSTRACT Reductions to 2, 5, and 42% of the wild-type transformation efficiency were found when Agrobacterium mutants carrying transposon insertions in virD2, virC2, and virE2, respectively, were used to transform Aspergillus awamori. The structures of the T-DNAs integrated into the host genome by these mutants were analyzed by Southern and sequence analyses. The T-DNAs of transformants obtained with the virE2 mutant had left-border truncations, whereas those obtained with the virD2 mutant had truncated right ends. From this analysis, it was concluded that the virulence proteins VirD2 and VirE2 are required for full-length T-DNA integration and that these proteins play a role in protecting the right and left T-DNA borders, respectively. Multicopy and truncated T-DNA structures were detected in the majority of the transformants obtained with the virC2 mutant, indicating that VirC2 plays a role in correct T-DNA processing and is required for single-copy T-DNA integration.

The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5′ mutations

Gene ◽  
1987 ◽  
Vol 53 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Catherine M. Corrick ◽  
Andrea P. Twomey ◽  
Michael J. Hynes
Keyword(s):  
Nucleotide Sequence ◽  
Aspergillus Nidulans ◽  
Molecular Characterization ◽  
Amds Gene

An Aspergillus nidulans uvsC null mutant is deficient in homologous DNA integration

2001 ◽  
Vol 264 (5) ◽  
pp. 709-715 ◽  
Author(s):  
D. Ichioka ◽  
T. Itoh ◽  
Y. Itoh
Keyword(s):  
Aspergillus Nidulans ◽  
Null Mutant ◽  
Dna Integration

Yeast proteins can activate expression through regulatory sequences of the amdS gene of Aspergillus nidulans

1995 ◽  
Vol 246 (2) ◽  
pp. 223-227 ◽  
Author(s):  
Nathalie Bonnefoy ◽  
Jane Copsey ◽  
Michael J. Hynes ◽  
Mervl A. Davis
Keyword(s):  
Aspergillus Nidulans ◽  
Regulatory Sequences ◽  
Amds Gene

scholarly journals Nitrogen Metabolism and Growth Enhancement in Tomato Plants Challenged with Trichoderma harzianum Expressing the Aspergillus nidulans Acetamidase amdS Gene

2016 ◽  
Vol 7 ◽  
Author(s):  
Sara Domínguez ◽  
M. Belén Rubio ◽  
Rosa E. Cardoza ◽  
Santiago Gutiérrez ◽  
Carlos Nicolás ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Nitrogen Metabolism ◽  
Trichoderma Harzianum ◽  
Growth Enhancement ◽  
Tomato Plants ◽  
Amds Gene

Agrobacterium-mediated transformation of the filamentous fungus Aspergillus awamori

2008 ◽  
Vol 3 (10) ◽  
pp. 1671-1678 ◽  
Author(s):  
Caroline B Michielse ◽  
Paul J J Hooykaas ◽  
Cees A M J J van den Hondel ◽  
Arthur F J Ram
Keyword(s):  
Filamentous Fungus ◽  
Aspergillus Awamori ◽  
Agrobacterium Mediated Transformation

Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori

2004 ◽  
Vol 41 (5) ◽  
pp. 571-578 ◽  
Author(s):  
C.B. Michielse ◽  
A.F.J. Ram ◽  
P.J.J. Hooykaas ◽  
C.A.M.J.J.van den Hondel
Keyword(s):  
Bacterial Virulence ◽  
Aspergillus Awamori ◽  
Agrobacterium Mediated Transformation ◽  
Virulence Proteins

scholarly journals How does the cell count the number of ectopic copies of a gene in the premeiotic inactivation process acting in Ascobolus immersus?

Genetics ◽  
1990 ◽  
Vol 124 (3) ◽  
pp. 585-591 ◽  
Author(s):  
G Faugeron ◽  
L Rhounim ◽  
J L Rossignol
Keyword(s):  
Cell Count ◽  
Aspergillus Nidulans ◽  
Repeated Sequences ◽  
Duplicated Genes ◽  
Recombinant Strains ◽  
Integrative Transformation ◽  
Ascobolus Immersus ◽  
Multiple Copies ◽  
Amds Gene ◽  
Inactivation Process

Abstract Repeated genes, artificially introduced in Ascobolus immersus by integrative transformation, are frequently inactivated during the sexual phase. Inactivation is observed in about 50% of meioses if duplicated genes are at ectopic chromosomal locations, and in 90% of meioses if genes are tandemly repeated. Inactivation is associated with extensive methylation of the cytosine residues of the duplicated sequences and is induced in the still haploid nuclei of the dikaryotic cell which will undergo karyogamy and subsequent meiosis. Only repeated sequences become methylated. This raises the intriguing question of how the premeiotic inactivation machinery is informed that a nucleus contains multiple copies of a gene. By using in crosses recombinant strains of A. immersus in which either one, two or three genetically independent copies of the exogenous amdS gene from Aspergillus nidulans had been introduced, we could follow the premeiotic inactivation of each one of the ectopic amdS copies. This led us to propose that a prerequisite for inactivation is a premeiotic pairing of repeated sequences and that each copy can undergo successive cycles of pairing. In fact, once methylated, a copy can pair with a still unmethylated copy, so that an uneven number of copies can be subject to inactivation.

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