Three inoculation methods, including cutting of a leaf, drenching, and point inoculation, were compared in an effort to screen the susceptibility of Chinese cabbage to soft rot disease caused by Erwinia carotovora subsp. carotovora. Three- to 4-week-old seedlings from 10 lines of cabbage with 16-h-old bacterial culture were routinely used. Inoculated seedlings were kept at 25.0 ± 10.0 °C for 48 h with saturated water vapor using a plastic tunnel in a greenhouse. Sixty-day-old mature plants were produced and inoculated in a greenhouse. Severity of symptoms, which were observed from percentage of plant infected was scored as from 1 to 9, representing resistant to susceptible, respectively. The correlation between seedlings and mature plants from ten lines was evaluated among the three different inoculation methods. Point inoculation gave the most significant correlation (r = 0.843, P < 0.05) between seedlings and mature plants. A good correlation was also observed between point inoculation of seedlings and drenching of mature plants (r = 0.609, P < 0.05). Cutting of a leaf of seedlings was also correlated with point inoculation of mature plants (r = 0.609, P < 0.05). This method provides the advantage of being able to keep the experimental plant alive, as only one leaf is detached. The point inoculation method is simple and relatively sensitive, so it could be used for large-scale screening for this bacterial soft rot disease. From three different evaluation assays, it was concluded that the breeding lines, C3-28, C3-29 from Cornell Univ. (Geneva, N.Y.) and the cultivar Kweonsim319 were relatively resistant to bacterial soft rot, while the Cornell breeding line CC-25 and the `Rang-no' cultivar were relatively susceptible.
Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
