Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate

It is known that cells contain various uncommon nucleotides such as dinucleoside polyphosphates (NpnN’s) and adenosine 5′-phosphoramidate (NH2-pA) belonging to nucleoside 5′-phosphoramidates (NH2-pNs). Their cellular levels are enzymatically controlled. Some of them are accumulated in cells under stress, and therefore, they could act as signal molecules. Our previous research carried out in Arabidopsis thaliana and grape (Vitis vinifera) showed that NpnN’s induced the expression of genes in the phenylpropanoid pathway and favored the accumulation of their products, which protect plants against stress. Moreover, we found that NH2-pA could play a signaling role in Arabidopsis seedlings. Data presented in this paper show that exogenously applied purine (NH2-pA, NH2-pG) and pyrimidine (NH2-pU, NH2-pC) nucleoside 5′-phosphoramidates can modify the expression of genes that control the biosynthesis of both stilbenes and lignin in Vitis vinifera cv. Monastrell suspension-cultured cells. We investigated the expression of genes encoding for phenylalanine ammonia-lyase (PAL1), cinnamate-4-hydroxylase (C4H1), 4-coumarate:coenzyme A ligase (4CL1), chalcone synthase (CHS1), stilbene synthase (STS1), cinnamoyl-coenzyme A:NADP oxidoreductase (CCR2), and cinnamyl alcohol dehydrogenase (CAD1). Each of the tested NH2-pNs also induced the expression of the trans-resveratrol cell membrane transporter VvABCG44 gene and caused the accumulation of trans-resveratrol and trans-piceid in grape cells as well as in the culture medium. NH2-pC, however, evoked the most effective induction of phenylpropanoid pathway genes such as PAL1, C4H1, 4CL1, and STS1. Moreover, this nucleotide also induced at short times the accumulation of N-benzoylputrescine (BenPut), one of the phenylamides that are derivatives of phenylpropanoid and polyamines. The investigated nucleotides did not change either the lignin content or the cell dry weight, nor did they affect the cell viability throughout the experiment. The results suggest that nucleoside 5′-phosphoramidates could be considered as new signaling molecules.

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Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds

International Journal of Molecular Sciences ◽

10.3390/ijms20205180 ◽

2019 ◽

Vol 20 (20) ◽

pp. 5180 ◽

Cited By ~ 5

Author(s):

Pascual García-Pérez ◽

Sonia Losada-Barreiro ◽

Pedro P. Gallego ◽

Carlos Bravo-Díaz

Keyword(s):

Bioactive Compounds ◽

Culture Medium ◽

Cultured Cells ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Extracellular Production ◽

Suspension Cultured Cells ◽

Culture Growth ◽

Cell Culture Growth

The rates of production of secondary metabolites obtained by employing conventional plant breeding may be low for practical purposes. Thus, innovative approaches for increasing their rates of production are being developed. Here, we propose the use of elicited plant suspension cultured cells (PSCC) with cyclodextrins (CDs) as an alternative method for the production of bioactive compounds from Bryophyllum species. For this purpose, we analyzed the effects of methyl–β–cyclodextrin and 2–hydroxypropyl–β–cyclodextrin on cell culture growth and on the intra- and extracellular production of phenols and flavonoids. Results clearly show that CDs enhance the biosynthesis of polyphenols by PSCC favoring their accumulation outside the cells. CDs shift the homeostatic equilibrium by complexing extracellular phenolics, causing stress in cells that respond by increasing the production of intracellular phenolics. We also analyzed the radical scavenging activity of the culture medium extracts against 2,2–diphenyl–1–pycrilhydrazyl (DPPH) radical, which increased with respect to the control samples (no added CDs). Our results suggest that both the increase in the production of polyphenols and their radical scavenging activity are a consequence of their inclusion in the CD cavities. Overall, based on our findings, CDs can be employed as hosts for increasing the production of polyphenols from Bryophyllum species.

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Diadenosine triphosphate is a novel factor which in combination with cyclodextrins synergistically enhances the biosynthesis of trans -resveratrol in Vitis vinifera cv. Monastrell suspension cultured cells

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2014.09.019 ◽

2014 ◽

Vol 84 ◽

pp. 271-276 ◽

Cited By ~ 10

Author(s):

Małgorzata Pietrowska-Borek ◽

Łukasz Czekała ◽

Sarai Belchí-Navarro ◽

María Angeles Pedreño ◽

Andrzej Guranowski

Keyword(s):

Vitis Vinifera ◽

Cultured Cells ◽

Suspension Cultured Cells ◽

Diadenosine Triphosphate

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Induction of freezing tolerance in potato (Solanum commersonii) suspension cultured cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1992.840107.x ◽

1992 ◽

Vol 84 (1) ◽

pp. 41-48 ◽

Cited By ~ 1

Author(s):

Stephen P. Lee ◽

Baolong Zhu ◽

Tony H. H. Chen ◽

Paul H. Li

Keyword(s):

Freezing Tolerance ◽

Cultured Cells ◽

Solanum Commersonii ◽

Suspension Cultured Cells

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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