Nucleoside 5′-Phosphoramidates Control the Phenylpropanoid Pathway in Vitis vinifera Suspension-Cultured Cells

It is known that cells contain various uncommon nucleotides such as dinucleoside polyphosphates (NpnN’s) and adenosine 5′-phosphoramidate (NH2-pA) belonging to nucleoside 5′-phosphoramidates (NH2-pNs). Their cellular levels are enzymatically controlled. Some of them are accumulated in cells under stress, and therefore, they could act as signal molecules. Our previous research carried out in Arabidopsis thaliana and grape (Vitis vinifera) showed that NpnN’s induced the expression of genes in the phenylpropanoid pathway and favored the accumulation of their products, which protect plants against stress. Moreover, we found that NH2-pA could play a signaling role in Arabidopsis seedlings. Data presented in this paper show that exogenously applied purine (NH2-pA, NH2-pG) and pyrimidine (NH2-pU, NH2-pC) nucleoside 5′-phosphoramidates can modify the expression of genes that control the biosynthesis of both stilbenes and lignin in Vitis vinifera cv. Monastrell suspension-cultured cells. We investigated the expression of genes encoding for phenylalanine ammonia-lyase (PAL1), cinnamate-4-hydroxylase (C4H1), 4-coumarate:coenzyme A ligase (4CL1), chalcone synthase (CHS1), stilbene synthase (STS1), cinnamoyl-coenzyme A:NADP oxidoreductase (CCR2), and cinnamyl alcohol dehydrogenase (CAD1). Each of the tested NH2-pNs also induced the expression of the trans-resveratrol cell membrane transporter VvABCG44 gene and caused the accumulation of trans-resveratrol and trans-piceid in grape cells as well as in the culture medium. NH2-pC, however, evoked the most effective induction of phenylpropanoid pathway genes such as PAL1, C4H1, 4CL1, and STS1. Moreover, this nucleotide also induced at short times the accumulation of N-benzoylputrescine (BenPut), one of the phenylamides that are derivatives of phenylpropanoid and polyamines. The investigated nucleotides did not change either the lignin content or the cell dry weight, nor did they affect the cell viability throughout the experiment. The results suggest that nucleoside 5′-phosphoramidates could be considered as new signaling molecules.

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Purine and pyrimidine dinucleoside polyphosphates differentially affect the phenylpropanoid pathway in Vitis vinifera L. cv. Monastrell suspension cultured cells

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2019.12.015 ◽

2020 ◽

Vol 147 ◽

pp. 125-132 ◽

Cited By ~ 2

Author(s):

Małgorzata Pietrowska-Borek ◽

Anna Wojdyła-Mamoń ◽

Jędrzej Dobrogojski ◽

Agnieszka Młynarska-Cieślak ◽

Marek R. Baranowski ◽

...

Keyword(s):

Vitis Vinifera ◽

Cultured Cells ◽

Phenylpropanoid Pathway ◽

Vitis Vinifera L ◽

Suspension Cultured Cells ◽

Dinucleoside Polyphosphates

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Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate

Plant Cell Reports ◽

10.1007/s00299-011-1141-8 ◽

2011 ◽

Vol 31 (1) ◽

pp. 81-89 ◽

Cited By ~ 80

Author(s):

Sarai Belchí-Navarro ◽

Lorena Almagro ◽

Diego Lijavetzky ◽

Roque Bru ◽

María A. Pedreño

Keyword(s):

Vitis Vinifera ◽

Cultured Cells ◽

Extracellular Production ◽

Suspension Cultured Cells

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Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2015.10.025 ◽

2015 ◽

Vol 97 ◽

pp. 361-367 ◽

Cited By ~ 26

Author(s):

Lorena Almagro ◽

Sarai Belchí-Navarro ◽

Ascensión Martínez-Márquez ◽

Roque Bru ◽

María A. Pedreño

Keyword(s):

Vitis Vinifera ◽

Cultured Cells ◽

Extracellular Production ◽

Suspension Cultured Cells

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Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell

Journal of Plant Physiology ◽

10.1016/j.jplph.2012.10.003 ◽

2013 ◽

Vol 170 (3) ◽

pp. 258-264 ◽

Cited By ~ 16

Author(s):

Sarai Belchí-Navarro ◽

Lorena Almagro ◽

Ana Belén Sabater-Jara ◽

Francisco Fernández-Pérez ◽

Roque Bru ◽

...

Keyword(s):

Vitis Vinifera ◽

Cultured Cells ◽

Pathogenesis Related Proteins ◽

Suspension Cultured Cells ◽

Pathogenesis Related ◽

Related Proteins

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Assessment of potential winter hardiness in winter bread wheat cultivars and genotypes by analyzing autofluorescence in seedling tissues

PROCEEDINGS ON APPLIED BOTANY GENETICS AND BREEDING ◽

10.30901/2227-8834-2021-1-33-40 ◽

2021 ◽

Vol 182 (1) ◽

pp. 33-40

Author(s):

I. V. Karkhardin ◽

A. A. Konovalov ◽

N. P. Goncharov

Keyword(s):

Winter Wheat ◽

Bread Wheat ◽

Lignin Content ◽

Fluorescence Analysis ◽

Cinnamyl Alcohol Dehydrogenase ◽

Frost Tolerance ◽

Phenylpropanoid Pathway ◽

Winter Hardiness ◽

Wheat Cultivars ◽

Winter Bread Wheat

Background.Winter crops are the most productive component of agricultural biocenoses. In Russia, winter wheat suffers the greatest losses in winter, so a search for traits marking high or low winter hardiness in autumn-sown genotypes, including improved cultivars, is needed to assess their potential for overwintering. One of such markers of high winter hardiness is an increased lignin content in plant tissues. The terminal enzyme in the phenylpropanoid pathway of metabolism, wherein lignin components are formed, is cinnamyl-alcohol dehydrogenase (CAD, EC 1.1.1.195). In plants, the CAD enzyme is one of the links in the aromatic metabolism, which generates, in addition to lignin, a number of aromatic compounds, such as lignans, aromatic glycosides, etc. Many of these compounds, like lignin, contain chromophore groups and are capable of autofluorescence.Correlations of the genotypes that incorporate CAD1-F with overwintering are studied in this work.Materials and methods.The winter bread wheat cultivars ‘Zitnica’ (Yugoslavia) and ‘Novosibirskaya 9’ (ICG SB RAS, Russia), contrasting in winter hardiness and CAD isozyme spectra, their hybrids, and 28 improved winter cultivars developed in Krasnodar were selected for the study. Fluorescence analysis of 28 winter wheat cultivars was also performed. Correlation coefficients between fluorescence and frost tolerance were calculated using the results of the analysis of 7 most contrasting cultivars.Conclusions. The tested winter bread wheat genotypes demonstrated the interplay between CAD1-F and successful overwintering: a correlation was found in the genotypes carrying the 00 CAD1-F allele with higher percentage of overwintered plants. This dependence was not observed in every season. The analysis of seedling sections for fluorescence can also be used for preliminary assessment of winter tolerance in winter bread wheat under laboratory conditions.

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Correlation Analysis of Lignin Accumulation and Expression of Key Genes Involved in Lignin Biosynthesis of Ramie (Boehmeria nivea)

Genes ◽

10.3390/genes10050389 ◽

2019 ◽

Vol 10 (5) ◽

pp. 389 ◽

Cited By ~ 3

Author(s):

Yinghong Tang ◽

Fang Liu ◽

Hucheng Xing ◽

Kaiquan Mao ◽

Guo Chen ◽

...

Keyword(s):

Natural Fiber ◽

Lignin Content ◽

Phenylpropanoid Pathway ◽

Maturation Period ◽

Expression Levels ◽

Relative Expression ◽

Lignin Synthesis ◽

Boehmeria Nivea ◽

Expression Of Genes ◽

Key Genes

The phloem of the stem of ramie (Boehmeria nivea) is an important source of natural fiber for the textile industry. However, the lignin content in the phloem affects the quality of ramie phloem fiber. In this study, the lignin content and related key gene expression levels were analyzed in the phloem and xylem at different developmental periods. The results showed that the relative expression levels of lignin synthesis-related key genes in the xylem and phloem of the stem gradually decreased from the fast-growing period to the late maturation period, but the corresponding lignin content increased significantly. However, the relative expression levels of a few genes were the highest during the maturation period. During all three periods, the lignin content in ramie stems was positively correlated with the expression of genes, including PAL, C4H and 4CL1 in the phenylpropanoid pathway, F5H and CCoAOMT in the lignin-specific synthetic pathway, and CAD in the downstream pathway of lignin synthesis, but the lignin content was negatively correlated with the expression of genes including 4CL3 in the phenylpropanoid pathway and UDP-GT in the shunt pathway of lignin monomer synthesis. The ramie 4CL3 recombinant protein prefers cinnamic acid as a substrate during catalysis, and it negatively regulates lignin synthesis. It is speculated that ramie 4CL3 is mainly involved in the synthesis of ramie flavonoid compounds, and that 4CL1 is mainly involved in lignin synthesis.

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Diadenosine triphosphate is a novel factor which in combination with cyclodextrins synergistically enhances the biosynthesis of trans -resveratrol in Vitis vinifera cv. Monastrell suspension cultured cells

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2014.09.019 ◽

2014 ◽

Vol 84 ◽

pp. 271-276 ◽

Cited By ~ 10

Author(s):

Małgorzata Pietrowska-Borek ◽

Łukasz Czekała ◽

Sarai Belchí-Navarro ◽

María Angeles Pedreño ◽

Andrzej Guranowski

Keyword(s):

Vitis Vinifera ◽

Cultured Cells ◽

Suspension Cultured Cells ◽

Diadenosine Triphosphate

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Biochemical Changes in Leaves of Vitis vinifera cv. Sangiovese Infected by Bois Noir Phytoplasma

Pathogens ◽

10.3390/pathogens9040269 ◽

2020 ◽

Vol 9 (4) ◽

pp. 269 ◽

Cited By ~ 1

Author(s):

Carmine Negro ◽

Erika Sabella ◽

Francesca Nicolì ◽

Roberto Pierro ◽

Alberto Materazzi ◽

...

Keyword(s):

Vitis Vinifera ◽

Defense Mechanism ◽

Lignin Content ◽

Soluble Sugars ◽

Phenylpropanoid Pathway ◽

Biochemical Changes ◽

Total Phenolic ◽

Vitis Vinifera L ◽

Bois Noir ◽

Grapevine Cultivar

Bois noir is a disease associated with the presence of phytoplasma ‘Candidatus Phytoplasma solani’ belonging to the Stolbur group (subgroup 16SrXII-A), which has a heavy economic impact on grapevines. This study focused on the changes induced by phytoplasma in terms of the profile and amount of secondary metabolites synthesized in the phenylpropanoid pathway in leaves of Vitis vinifera L. red-berried cultivar Sangiovese. Metabolic alterations were assessed according to the disease progression through measurements of soluble sugars, chlorophyll, and phenolic compounds produced by plant hosts, in response to disease on symptomatic and asymptomatic Bois noir-positive plants. Significant differences were revealed in the amount of soluble sugars, chlorophyll, and accumulation/reduction of some compounds synthesized in the phenylpropanoid pathway of Bois noir-positive and negative grapevine leaves. Our results showed a marked increase in phenolic and flavonoid production and a parallel decrease in lignin content in Bois noir-positive compared to negative leaves. Interestingly, some parameters (chlorophyll a, soluble sugars, total phenolic or flavonoids content, proanthocyanidins, quercetin) differed between Bois noir-positive and negative leaves regardless of symptoms, indicating measurable biochemical changes in asymptomatic leaves. Our grapevine cultivar Sangiovese results highlighted an extensive modulation of the phenylpropanoid biosynthetic pathway as a defense mechanism activated by the host plant in response to Bois noir disease.

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Induction of freezing tolerance in potato (Solanum commersonii) suspension cultured cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1992.840107.x ◽

1992 ◽

Vol 84 (1) ◽

pp. 41-48 ◽

Cited By ~ 1

Author(s):

Stephen P. Lee ◽

Baolong Zhu ◽

Tony H. H. Chen ◽

Paul H. Li

Keyword(s):

Freezing Tolerance ◽

Cultured Cells ◽

Solanum Commersonii ◽

Suspension Cultured Cells

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Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

10.26686/wgtn.12597854.v1 ◽

2020 ◽

Author(s):

Ian Sims ◽

K Middleton ◽

AG Lane ◽

AJ Cairns ◽

A Bacic

Keyword(s):

Cultured Cells ◽

Oryza Sativa L ◽

Suspension Cultures ◽

Exchange Chromatography ◽

Phleum Pratense ◽

Arabinogalactan Protein ◽

Extracellular Polysaccharides ◽

Type Ii ◽

Hordeum Vulgare L ◽

Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

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