Polyamine biosynthesis during somatic embryogenesis in interior spruce (Picea glauca�Picea engelmannii complex)

1996 ◽  
Vol 15 (7) ◽  
pp. 495-499 ◽  
Author(s):  
Vindhya Amarasinghe ◽  
John E. Carlson ◽  
Rajwinder Dhami
Keyword(s):  
Somatic Embryogenesis ◽  
Picea Glauca ◽  
Picea Engelmannii ◽  
Polyamine Biosynthesis ◽  
Interior Spruce

Area-based breeding zones to minimize maladaptation

2004 ◽  
Vol 34 (3) ◽  
pp. 695-704 ◽  
Author(s):  
Gregory A O'Neill ◽  
Sally N Aitken
Keyword(s):  
Cluster Analysis ◽  
Picea Glauca ◽  
Grid Cell ◽  
Picea Engelmannii ◽  
Breeding Programs ◽  
Adaptive Value ◽  
Interior Spruce ◽  
Breeding Zone ◽  
Abc Analysis ◽  
Predetermined Number

A new breeding zone delineation scheme identifies for a given number of zones the zone-boundary placement that minimizes regional maladaptation in breeding programs. First, an adaptive map is created by using conventional genetic test data. Then, the large array of predicted adaptive values is subjected to cluster analysis, which assigns each grid cell of the region to one of a predetermined number of clusters (breeding zones) such that the sum of the squared distances between each cell's adaptive value and its cluster mean is minimized. This approach minimizes the average adaptive distance between the origin of a breeding program's selected trees and planting locations throughout the region of focus. The procedure is illustrated by the use of adaptive values of 69 interior spruce (Picea engelmannii Parry ex Engelm. × Picea glauca (Moench) Voss) open-pollinated families (sources) from southeast British Columbia, Canada. Adaptive values of each 1.5 km × 1.5 km grid cell in the 80 000-km2 region were predicted using a geneco logical model (R2 = 0.64), and the values were subjected to cluster analysis to identify breeding zone boundaries that were then mapped using a geographic information system. Regardless of the number of zones created, a regional maladaptation index was consistently smaller when zones were devised with area-based cluster (ABC) analysis than when zones were created by dividing the region into bands of equal elevational or adaptive-value widths. Application of the ABC procedure should assist in identifying the optimum breeding-zone alignment for a given number of zones.

Conservation and divergence of gene expression plasticity followingc. 140 million years of evolution in lodgepole pine (Pinus contorta) and interior spruce (Picea glauca × Picea engelmannii)

2014 ◽  
Vol 203 (2) ◽  
pp. 578-591 ◽  
Author(s):  
Sam Yeaman ◽  
Kathryn A. Hodgins ◽  
Haktan Suren ◽  
Kristin A. Nurkowski ◽  
Loren H. Rieseberg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Picea Glauca ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Picea Engelmannii ◽  
Interior Spruce

Nitrogen uptake and utilization by slow- and fast-growing families of interior spruce under contrasting fertility regimes

2003 ◽  
Vol 33 (6) ◽  
pp. 959-966 ◽  
Author(s):  
B D Miller ◽  
B J Hawkins
Keyword(s):  
Nitrogen Uptake ◽  
Picea Glauca ◽  
Dry Mass ◽  
Picea Engelmannii ◽  
Fertility Level ◽  
Fast Growing ◽  
Interior Spruce ◽  
Sib Families ◽  
Slow Growing ◽  
Mass Allocation

Six full-sib families of interior spruce (Picea glauca (Moench) Voss × Picea engelmannii Parry ex Engelm.) of contrasting growth rates (three fast-growing, three slow-growing families) were grown from seed in a greenhouse under three fertility regimes (25, 75, and 125 mg nitrogen/seedling over 175 days). The use of vector analysis showed that the lowest fertility regime was nitrogen-limited while the highest indicated luxury consumption. After 175 days, fast-growing families were larger than slow-growing at all fertility levels. At the lowest fertility level, fast-growing families exhibited greater nitrogen productivity and utilization of internal nitrogen. At higher fertility levels, fast-growing families took up nitrogen more quickly and efficiently thus accumulating greater nitrogen reserves. Fast-growing families also exhibited a greater plasticity in dry mass allocation between shoots and roots with different fertility levels.

scholarly journals Stock Quality Assessment of a Somatic Interior Spruce Seedlot

2005 ◽  
Vol 22 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Steven C. Grossnickle ◽  
Raymund Folk
Keyword(s):  
Somatic Embryogenesis ◽  
Root Growth ◽  
Quality Assessment ◽  
Vegetative Propagation ◽  
Picea Glauca ◽  
Growth Potential ◽  
Interior Spruce ◽  
Stock Quality ◽  
Similar Range ◽  
Assessment Performance

Abstract Somatic embryogenesis is a form of vegetative propagation that uses tissue culture procedures to rapidly multiply elite genotypes of a selected tree species. Somatic embryogenesis technology still is considered novel by the forest industry for use within forest regeneration programs. In this article, a stock quality testing approach was used to compare a somatic seedlot to comparable zygotic seedlots. The somatic seedlot was comprised of interior spruce (Picea glauca (Moench) Voss × Picea engelmannii Parry ex. Engelm.) crosses (PG × PG), white spruce (P. glauca (Moench) Voss) crosses (ENA × ENA), or a mixture thereof (PG × ENA or ENA × PG). This somatic seedlot was compared to zygotic orchard-collected seedlots that shared partial parentage with the somatic seedlot. Seedlings produced from all seedlots were container-grown under standard nursery cultural procedures. Height and rootcollar diameter were measured on all seedlots. Shoot growth potential (i.e., length of new leader elongation) and root growth capacity were measured under controlled environment conditions. Mean height and diameter of the somatic and zygotic seedlots fell within a similar range that met operational grading criteria for a plantable seedling. The somatic and zygotic seedlots also had similar shoot and root growth capability. Comparable stock quality assessment performance between the somatic and zygotic seedlots indicated that somatic embryogenesis is a viable vegetative propagation technology for producing seedlings suitable for reforestation programs. North. J. Appl. For. 22(3):197–202.

Comparisons of gas exchange parameters and shoot water relations of interior spruce (Picea glauca (Moench) Voss times Picea engelmannii Parry ex Engelm.) clones under repeated soil drought

1998 ◽  
Vol 28 (6) ◽  
pp. 820-830 ◽  
Author(s):  
Shihe Fan ◽  
Steven C Grossnickle
Keyword(s):  
Water Relations ◽  
Picea Glauca ◽  
Net Photosynthesis ◽  
Picea Engelmannii ◽  
Soil Drought ◽  
Gas Exchange Parameters ◽  
Tissue Water ◽  
Shoot Tissue ◽  
Interior Spruce ◽  
Exchange Parameters

Physiological response of interior spruce (Picea glauca (Moench) Voss x Picea engelmannii Parry ex Engelm.) to drought conditions was compared for somatic seedlings from clones G351, T703, N366, and W460. Seedlings were subjected to four cycles of progressive soil drought by withholding water. Changes in net photosynthesis (Pn), stomatal conductance (gwv), and predawn water potentials ( psi pd) were measured during and after each drought cycle. Shoot tissue water relations parameters were measured using pressure-volume analysis at the beginning and end of the fourth drought cycle. When comparing drought cycle 1 with drought cycle 4, clones G351, N366, and T703 showed significantly reduced Pn, but gwv of all clones was not affected. Net photosynthesis and gwv decreased with psi pd more rapidly in clone W460 than in the remaining clones. When rewatered after drought, clone T703 had the most rapid Pn and gwv recovery whereas clone G351 had the slowest recovery of Pn and gwv. Over four drought cycles, all clones photosynthesized at progressively lower psi pd, but adjustments in tissue water relations parameters were marginal. These results implied that gas exchange parameters were more sensitive than shoot tissue water relations parameters in detecting clonal variation in the physiological response of interior spruce under simulated drought conditions.

Effects of fertilization on resin canal defences and incidence of Pissodes strobi attack in interior spruce

2004 ◽  
Vol 34 (4) ◽  
pp. 855-862 ◽  
Author(s):  
Lara vanAkker ◽  
René I Alfaro ◽  
Robert Brockley
Keyword(s):  
Picea Glauca ◽  
Tree Height ◽  
Resin Canal ◽  
Picea Engelmannii ◽  
Bark Thickness ◽  
White Pine ◽  
Pissodes Strobi ◽  
Interior Spruce ◽  
Feasible Option ◽  
White Pine Weevil

The effects of six fertilization treatments on tree height and incidence of attack by the white pine weevil, Pissodes strobi (Peck), on interior spruce (a hybrid, Picea glauca (Moench) Voss × Picea engelmannii Parry ex Engelm.) were explored in a field study. In a corresponding laboratory study, changes in constitutive and traumatic resin canal defences in response to fertilization were measured. Incidence of weevil attack increased with fertilization intensity. This trend was explained by increased resources available for weevil feeding (adults and larvae) as a result of increased leader size and bark thickness, as well as by an observed weakening in the tree's constitutive resin canal defences. The ability of interior spruce to produce a traumatic resin response was not influenced by fertilization. Although incidence of weevil attack was greatest in trees from the most intense fertilization treatments, height losses due to weevil attack were not as great as height gains due to fertilization treatment. Therefore, we concluded that fertilization is a feasible option for increasing productivity of interior spruce plantations, particularly if other weevil control alternatives are implemented.

Genetic variation in summer gas exchange patterns of interior spruce (Picea glauca (Moench) Voss times Picea engelmannii Parry ex Engelm.)

1998 ◽  
Vol 28 (6) ◽  
pp. 831-840 ◽  
Author(s):  
Steven C Grossnickle ◽  
Shihe Fan
Keyword(s):  
Gas Exchange ◽  
Picea Glauca ◽  
Isotope Composition ◽  
Vapour Pressure Deficit ◽  
Net Photosynthesis ◽  
Picea Engelmannii ◽  
Gas Exchange Parameters ◽  
Exchange Processes ◽  
Intrinsic Water Use Efficiency ◽  
Interior Spruce

Interior spruce (Picea glauca (Moench) Voss x Picea engelmannii Parry ex Engelm.) somatic seedlings from a range of clones were measured for gas exchange processes in relation to summer atmospheric parameters. Carbon isotope composition ( delta 13C) of needles was compared with gas exchange parameters and intrinsic water use efficiency (WUEi). Needle conductance (gwv) decreased as vapour pressure deficit (VPD) increased. Clone T703 had the lowest gwv level across all VPD conditions, with clones G351, N366, and W460 having significantly greater gwv than clone T703 at VPD levels <2.0 kPa. Response of net photosynthesis (Pn) to photosynthetically active radiation (PAR) for all clones showed Pn to increase to PAR levels of around 1000 µmol ·m-2 ·s-1 and then, Pn rates were stable at higher PAR levels. Clones N366 and W460 had significantly greater Pn than clone T703 at all PAR levels. All clones showed Pn to decline as VPD increased, with clone W460 having the highest predicted Pn level across all VPD conditions and significantly greater Pn than clone T703. Higher WUEi was related to an increase in delta 13C, with clone T703 having the highest WUEi and delta 13C, followed by clone W460. Changes in delta 13C, for all clones, indicated a stronger relationship with gwv than with Pn.

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