Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time

1999 ◽  
Vol 19 (1) ◽  
pp. 32-36 ◽  
Author(s):  
J. M. Myers ◽  
P. W. Simon
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Conditions ◽  
Clonal Variation ◽  
Over Time

The Effects of Plant Growth Regulators and Culture Conditions on the Growth and the Alkaloid Content of Callus Cultures of Cinchona pubescens

Planta Medica ◽  
1982 ◽  
Vol 46 (09) ◽  
pp. 15-18 ◽  
Author(s):  
Th. Mulder-Krieger ◽  
R. Verpoorte ◽  
Y. de Graaf ◽  
M. van der Kreek ◽  
A. Baerheim-Svendsen
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Cultures ◽  
Culture Conditions ◽  
Alkaloid Content ◽  
Cinchona Pubescens

scholarly journals Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1155
Author(s):  
Júlia Hunková ◽  
Monika Szabóová ◽  
Alena Gajdošová
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Adventitious Shoots ◽  
Culture Conditions ◽  
Indirect Organogenesis ◽  
Adventitious Regeneration ◽  
Amelanchier Alnifolia ◽  
Indole 3 Acetic Acid

The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L.kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L. kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average).

Optimization of culture conditions for indole-3-pyruvic acid production by Streptomyces griseoflavus

1994 ◽  
Vol 40 (9) ◽  
pp. 754-760 ◽  
Author(s):  
Mohamed S. El-Abyad ◽  
Mostafa A. El-Sayed ◽  
Abdel-Reheem El-Shanshoury ◽  
Mervat Farid
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Pyruvic Acid ◽  
Culture Conditions ◽  
Diaminopimelic Acid ◽  
Genus Streptomyces ◽  
Wide Range ◽  
The Family ◽  
Maximal Production

Thirty strains of actinomycetes were isolated from fertile soils in Egypt on starch–nitrate agar. The isolated organisms were recognized as spore formers with aerial mycelia containing L-DAP (diaminopimelic acid) and glycine. The inability of the vegetative mycelia to fragment into bacillary or coccoid forms and the presence of spores borne on sporophores, placed these organisms in the family Streptomycetaceae. The isolates also proved to be aerobic and Gram positive. The absence of DL-DAP and the presence of L-DAP in their cell hydrolysates, and the production of a wide range of pigments in the aerial and substrate mycelia, assigned these isolates to the genus Streptomyces. The isolated organisms were screened for production of plant growth regulators, and the most active producer of indole-3-pyruvic acid was examined further. Using the described morphological, physiological, and biochemical criteria for identification, this organism was identified as Streptomyces griseoflavus. The optimal culture conditions for maximal production of both cellular and extracellular indole-3-pyruvic acid by S. griseoflavus were investigated and shown to be as follows: a culture medium composed of (g∙100 mL−1) NH4NO3, 0.55; DL-tryptophan, 0.4; NaCl, 0.7; at pH 7 (buffered); with an incubation period of 6 days at 28 °C in the dark and under shaking conditions.Key words: actinomycetes, plant growth regulators, Streptomyces griseoflavus, indole-3-pyruvic acid, culture conditions.

Wheat regenerants in the system of rapid assessment of the effect of 24-epibrassinolide

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 394-397
Author(s):  
Seldimirova O.A. ◽  
M.V. Bezrukova ◽  
N.N. Кruglova ◽  
F.М. Shakirova
Keyword(s):  
Plant Growth ◽  
Experimental Model ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Drought Resistance ◽  
Rapid Assessment ◽  
Immature Embryo ◽  
Model System ◽  
Wheat Cultivars ◽  
Experimental Model System

The influence of 24-epibrassinolide on the efficiency of regenerants obtained from embryonic calli formation was studied in wheat cultivars contrast for drought resistance. The possibility of using the experimental model system «immature embryo – embryonic callus – regenerant» in the rapid assessment of the effect of antistress plant growth regulators is shown.

Mitochondria functional state of pea seedling in conditions of water deficit and treatment with plant growth regulators

2017 ◽  
Vol 2017 (1) ◽  
pp. 69-80
Author(s):  
I. V. Zhigacheva ◽  
◽  
V. I. Binukov ◽  
E. M. Mil’ ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Water Deficit ◽  
Plant Growth Regulators ◽  
Functional State ◽  
Growth Regulators ◽  
Pea Seedling
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