Applications of immuno-magnetic bead and immunofluorescent flow cytometric techniques for the quantitative detection of HAB microalgae

2011 ◽  
Vol 30 (3) ◽  
pp. 433-439 ◽  
Author(s):  
Jian Huang ◽  
Ruobing Wen ◽  
Zhenmin Bao ◽  
Zhenghong Sui ◽  
Ningbo Sun ◽  
...  
Keyword(s):  
Magnetic Bead ◽  
Quantitative Detection ◽  
Flow Cytometric

scholarly journals Magnetic Bead-Based Immunoassay Allows Rapid, Inexpensive, and Quantitative Detection of Human SARS-CoV-2 Antibodies

ACS Sensors ◽  
2021 ◽  
Author(s):  
Luciano F. Huergo ◽  
Khaled A. Selim ◽  
Marcelo S. Conzentino ◽  
Edileusa C. M. Gerhardt ◽  
Adrian R. S. Santos ◽  
...  
Keyword(s):  
Magnetic Bead ◽  
Quantitative Detection

Interference-free SERS tags for ultrasensitive quantitative detection of tyrosinase in human serum based on magnetic bead separation

2020 ◽  
Vol 1138 ◽  
pp. 150-157
Author(s):  
Dechan Lu ◽  
Xueliang Lin ◽  
Cairou Chen ◽  
Yudong Lu ◽  
Shangyuan Feng ◽  
...  
Keyword(s):  
Human Serum ◽  
Magnetic Bead ◽  
Quantitative Detection ◽  
Magnetic Bead Separation ◽  
Sers Tags

scholarly journals Rapid response flow cytometric assay for the detection of antibody responses to SARS-CoV-2

2020 ◽  
Author(s):  
Dennis Lapuente ◽  
Clara Maier ◽  
Pascal Irrgang ◽  
Julian Hübner ◽  
Antonia Sophia Peter ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Viral Infections ◽  
Rapid Development ◽  
Antibody Responses ◽  
Quantitative Detection ◽  
Flow Cytometric Assay ◽  
Serological Tests ◽  
Diagnostic Assays ◽  
Flow Cytometric ◽  
External Standard

Abstract SARS-CoV-2 has emerged as a previously unknown zoonotic coronavirus that spread worldwide causing a serious pandemic. While reliable nucleic acid–based diagnostic assays were rapidly available, only a limited number of validated serological assays were available in the early phase of the pandemic. Here, we evaluated a novel flow cytometric approach to assess spike-specific antibody responses.HEK 293T cells expressing SARS-CoV-2 spike protein in its natural confirmation on the surface were used to detect specific IgG and IgM antibody responses in patient sera by flow cytometry. A soluble angiotensin-converting-enzyme 2 (ACE-2) variant was developed as external standard to quantify spike-specific antibody responses on different assay platforms. Analyses of 201 pre-COVID-19 sera proved a high assay specificity in comparison to commercially available CLIA and ELISA systems, while also revealing the highest sensitivity in specimens from PCR-confirmed SARS-CoV-2-infected patients. The external standard allowed robust quantification of antibody responses among different assay platforms. In conclusion, our newly established flow cytometric assay allows sensitive and quantitative detection of SARS-CoV-2-specific antibodies, which can be easily adopted in different laboratories and does not rely on external supply of assay kits. The flow cytometric assay also provides a blueprint for rapid development of serological tests to other emerging viral infections

scholarly journals Phosphorylation of histone H2AX as an indicator of received dose of gamma radiation after whole-body irradiation of rats

2011 ◽  
Vol 80 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Radim Havelek ◽  
Martina Řezáčová ◽  
Zuzana Šinkorová ◽  
Lenka Zárybnická ◽  
Aleš Tichý ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Whole Body ◽  
Quantitative Detection ◽  
Atm Kinase ◽  
Strand Breaks ◽  
Histone H2ax ◽  
Flow Cytometric Method ◽  
Flow Cytometric

The aim of our study was to determine whether phosphorylation of histone H2AX can be used as an indicator of received dose of gamma radiation after whole-body irradiation of rats. Wistar rats were irradiated by 1-10 Gy of gamma radiation by60Co source. Value LD50/60 was 7.37 (4.68-8.05) Gy. Histone H2AX is phosphorylated by ATM kinase on serine 139 (γH2AX) quickly after the irradiation. It forms microscopically visible foci in the site of double strand breaks of DNA. Flow-cytometric method was used for quantitative detection. This study is the first one that evaluated dose-dependency of H2AX phosphorylation in peripheral lymphocytes of rats irradiated by whole-body dose 1-10 Gy. Our data show a dose-dependent increase in γH2AX in rat peripheral blood lymphocytes 1 h after whole-body irradiation by the dose of 1-10 Gy. We proved that phosphorylation of histone H2AX is a prompt and reliable indicator of the received radiation dose suitable for rapid measurement before the number of lymphocytes in peripheral blood starts to decrease. It can be used already 1 h after the irradiation for an estimation of the received dose of radiation. Blood samples can be stored in 4 °C for 23 h without significantly affecting the result.

scholarly journals A multi-center clinical study comparing Sansure Magb and CAP/CTM HBV tests in the quantitative detection of HBV DNA

2016 ◽  
Vol 10 (07) ◽  
pp. 755-761 ◽  
Author(s):  
Xiaoyu Fu ◽  
Deming Tan ◽  
Xiaoguang Dou ◽  
Jinjun Chen ◽  
Juan Wu
Keyword(s):  
Developing Countries ◽  
Accurate Method ◽  
Magnetic Bead ◽  
Hbv Dna ◽  
Quantitative Detection ◽  
Plot Analysis ◽  
Highly Sensitive ◽  
Data Points ◽  
Quantitative Results ◽  
Chinese Company

Introduction: As the most reliable means of diagnosing hepatitis (HBV) infection and predicting the prognosis of HBV-related chronic liver disease, the COBAS AmpliPrep/COBAS TaqMan real-time polymerase chain reaction (PCR) (CAP/CTM) assay provides a highly sensitive and accurate method for quantifying HBV DNA. However, the high cost of the COBAS reagents is prohibitive in many developing countries. Thus, we compared the Sansure magnetic bead (Magb) assay, a novel technology developed by a Chinese company, with the CAP/CTM assay. Methodology: The reproducibility and sensitivity of the Sansure Magb assay were first validated using HBV DNA reference samples. Next, the quantitative results for the two assays using 635 blood samples collected from chronic hepatitis B patients and 10 healthy controls were compared. Results: The Sansure Magb assay showed high reproducibility and was at least as sensitive and specific as the CAP/CTM assay. Among the patient samples, 407 tested positive by both methods, with 386 (94.84%) showing quantitative differences of less than 1 log unit and 21 (5.16%) showing quantitative differences of between 1 and 2 log units. The results from the assays were closely correlated. Bland-Altman plot analysis showed that only 6.6% of the data points fell outside the 95% limits of agreement, which suggests that the differences between methods are clinically acceptable. Conclusions: This study demonstrates that the Sansure Magb assay is highly sensitive and reproducible. Based on its reduced cost, the Sansure Magb assay may be more applicable than the CAP/CTM assay for HBV diagnosis in developing countries such as China.

scholarly journals Differentiation of normal human pre-B cells in vitro.

1990 ◽  
Vol 172 (1) ◽  
pp. 325-334 ◽  
Author(s):  
J G Villablanca ◽  
J M Anderson ◽  
M Moseley ◽  
C L Law ◽  
R L Elstrom ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Flow Cytometric Analysis ◽  
Magnetic Bead ◽  
B Cell Differentiation ◽  
Differentiated Cells ◽  
Flow Cytometric ◽  
Normal Human

The differentiation of surface Ig- pre-B cells into surface Ig+ B cells is a critical transition in mammalian B cell ontogeny. Elucidation of the growth factor requirements and differentiative potential of human pre-B cells has been hampered by the absence of a reproducible culture system that supports differentiation. Fluorescence-activated cell sorting and magnetic bead depletion were used to purify fetal bone marrow CD10+/surface mu- cells, which contain 60-70% cytoplasmic mu+ pre-B cells. CD10+/surface mu- cells cultured for 2 d were observed to differentiate into surface mu+ cells. Analysis by Southern blotting provided direct evidence that rearrangement of kappa light chain genes occurs in culture, and flow cytometric analysis revealed the appearance of surface Ig+ B cells expressing mu/kappa or mu/lambda. Unexpectedly, the kappa/lambda ratio in differentiated cells was the inverse of what is normally observed in adult peripheral blood. Differentiation occurs in the absence of exogenous growth factors or cytokines, suggesting that a stimulus-independent differentiative inertia might characterize pre-B cells in vivo. Future use of this model will facilitate our understanding of normal and abnormal human pre-B cell differentiation.

scholarly journals Establish a flow cytometric method for quantitative detection of Beclin-1 expression

2013 ◽  
Vol 65 (4) ◽  
pp. 481-489 ◽  
Author(s):  
Yuping He ◽  
Zhentao Mo ◽  
Zhongfeng Xue ◽  
Yongqi Fang
Keyword(s):  
Beclin 1 ◽  
Quantitative Detection ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals Rapid response flow cytometric assay for the detection of antibody responses to SARS-CoV-2

2020 ◽  
Author(s):  
Dennis Lapuente ◽  
Clara Maier ◽  
Pascal Irrgang ◽  
Julian Huebner ◽  
Sophia Antonia Peter ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Quantitative Detection ◽  
Converting Enzyme ◽  
Flow Cytometric Assay ◽  
Angiotensin Converting Enzyme 2 ◽  
Specific Antibodies ◽  
Diagnostic Assays ◽  
Flow Cytometric ◽  
External Standard ◽  
Specific Igg

SARS-CoV-2 has emerged as a previously unknown zoonotic coronavirus that spread worldwide causing a serious pandemic. While reliable nucleic acid-based diagnostic assays were rapidly available, there exists only a limited number of validated serological assays. Here, we evaluated a novel flow cytometric approach based on antigen-expressing HEK 293T cells to assess spike-specific IgG and IgM antibody responses. Analyses of 201 pre-COVID-19 sera proved a high assay specificity in comparison to commercially available CLIA and ELISA systems, while also revealing the highest sensitivity in specimens from PCR-confirmed SARS-CoV-2 infected patients. Additionally, a soluble Angiotensin-Converting-Enzyme 2 (ACE-2) variant was established as external standard to quantify spike-specific antibody responses on different assay platforms. In conclusion, our newly established flow cytometric assay allows sensitive and quantitative detection of SARS-CoV-2-specific antibodies, which can be easily adopted in different laboratories and does not rely on external supply of assay kits.

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